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| 1 | Micro RNA hsa-mi R-613 targets the human LXRαgene and mediates a feedback loop of LXRαautoregulation显示文摘 | Ou Z Wada T Gramignoli R | 2011 | Mol Endocrinol2011，4，： | 1 |
| 2 | Translation of amnion stem cells to the clinic显示文摘 | Strom SC Skvorak K Gramignoli R | 2013 | Stem Cells Dev2013，22，1： | 1 |
| 3 | Hepatobiliary disposition of 17-OHPC and taurocholate in fetal human hepatocytes: A comparison with adult human hepatocytes显示文摘 | Shringi Sharma Ewa CS Ellis Roberto Gramignoli | 2013 | Drug Metabolism and Disposition: The Bio- logical Fate of Chemicals2013，41，2： | 1 |
| 4 | Melatonin expresses powerful anti-inflammatory and antioxidant activities resulting in complete improvement of acetic-acid-induced colitis in rats 显示文摘 | Tahan G Gramignoli R Marongiu F | 2011 | Dig Dis Sci2011，56，3： | 1 |
| 5 | Hepatic differentiation of amniotic epithelial cells 显示文摘 | Marongiu F Gramignoli R Dorko K | 2011 | Hepatology2011，53，5： | 1 |
| 6 | Hepatobili- ary disposition of 17-OHPC and taurocholate in fetal human hepatocytes:A comparison with adult human hepatocytes显示文摘 | SHARMA S ELLIS EC GRAMIGNOLI R | 2013 | Drug Metabolism and Disposition2013，41，2： | 1 |
| 7 | Hepatic dif-ferentiation of amniotic epithelial cells显示文摘 | Marongiu F Gramignoli R Dorko K | 2011 | Hepatology2011，53，5： | 1 |
| 8 | Hepatic differentiation of amniotic epithelial cells显示文摘 | Marongiu F Gramignoli R Dorko K | | 0，，05： | 1 |
| 9 | MicroRNA hsa-miR- 613 targets the human LXRa gene and mediates a feed- back loop of LXRa autoregulation显示文摘 | Ou Z Wada T Gramignoli R | 2011 | Mol Endocrinol2011，25，4： | 1 |
| 10 | Strategies for short-term storage of hepatocytes for repeated clinical infusions显示文摘 | Jorns C Gramignoli R Saliem M | 2013 | Cell Transplant2013，29，： | 1 |
| 11 | Biofabrication of size-controlled liver microtissues incorporated with ECM-derived microparticles to prolong hepatocyte function显示文摘Multicellular microtissues of primary human hepatocytes(PHHs)co-cultured with other supporting cell types are a promis-ing model for drug screening and toxicological studies.However,these liver microtissues(LMs)rapidly lose their functions during ex vivo culture.Here,in order to mimic the cellular and structural hepatic microenvironment,we co-cultured PHHs with human mesenchymal stromal cells(MSCs)and human umbilical vein endothelial cells(HUVECs)in the presence of cell-sized microparticles(MPs)derived from liver extracellular matrix(LEMPs).The microwell culture platform enabled biofabrication of size-controlled multicellular microtissues(PHH:HUVEC:MSC=3:2:1)with efficient LEMP incorporation(about 70%at a 2:1 ratio of cells:MP).The biofabricated liver microtissues(BLMs)were cultured ex vivo for 14 days and compared to the cell-only LM in terms of gene and protein expression,functional activity,cytochrome P450(CYP450)enzyme inducibility,and drug sensitivity.The results supported superior hepatic-related gene expression,functional activity,and polarity for PHH in BLM compared to LM.CYP450 enzyme inducibility and dose-responsive sensitivity to toxic drugs were significantly higher in the BLM group.In conclusion,microtissue engineering by incorporation of tissue-specific microparticles within a multicellular microtissue can offer some advantages for drug discovery studies and cell transplantation applications.In the near future,this approach could generate a scalable platform of several functional biofabricated microtissues representing different organs. | Zahra Heydari Ibrahim Zarkesh Mohammad-Hossein Ghanian Mahdokht HAghdaei Svetlana Kotova Ensieh Zahmatkesh Zahra Farzaneh Abbas Piryaei Iman Akbarzadeh Anastasia Shpichka Roberto Gramignoli Peter Timashev Hossein Baharvand Massoud Vosough | 2021 | Bio-Design and Manufacturing2021，4，4： | 0 |
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