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| 1 | Atomic force microscopy correlates antimetastatic potentials of HepG2 cell line with its redox/energy status: effects of curcumin and Khaya senegalensis显示文摘OBJECTIVE: The fatality of cancer is mostly dependent on the possibility of occurrence of metastasis. Thus, if the development of metastasis can be prevented through novel therapeutic strategies targeted against this process, then the success of cancer treatment will drastically increase. In this study, therefore, we evaluated the antimetastatic potentials of an extract of Khaya senegalensis and curcumin on the metastatic liver cell line HepG2, and also assessed the anticancer property of the extract.METHODS: Cells were cultured and treated with graded concentrations of test substances for 24, 48, or 72 h with provisions made for negative controls. Treated cells were assessed as follows: nanotechnologically—atomic force microscopy(AFM) was used to determine cell stiffness; biochemically—cell cytotoxicity, glutathione level and adenosine triphosphate status, caspase activation and mitochondrial toxicity were considered; and microbiologically—a carrot disk assay was used to assess the anticancer property of the extract of K. senegalensis.RESULTS: Curcumin and K. senegalensis increased the cell stiffness by 2.6-and 4.0-fold respectively, indicating their antimetastatic effects. Corresponding changes in redox(glutathione level) and energy(adenosine triphosphate) status of the cells were also demonstrated. Further mechanistic studies indicated that curcumin was not mitotoxic in HepG2 cells unlike the K. senegalensis extract. In addition, the extract potently inhibited the Agrobacterium tumefaciens-induced genetic transformation based on carrot disk assay.CONCLUSION: Cell elasticity measurement data, using AFM, strongly suggested, for the first time, that both curcumin and the extract of K. senegalensis exhibited antimetastatic properties on HepG2 cells. | Jeremiah Olorunjuwon Olugbami Robert Damoiseaux Bryan France Michael A. Gbadegesin Adam Z. Stieg Shivani Sharma Oyeronke A. Odunola James K. Gimzewski | 2017 | Journal of Integrative Medicine2017，15，3： | 2 |
| 2 | Nanoscale science of single molecules using local probes显示文摘 | Gimzewski J K Joachim C | 1999 | Science1999，283，： | 1 |
| 3 | One-dimensional metal structures at decorated steps 显示文摘 | Jung T Schlittler R Gimzewski J K | 1995 | Appl Phys A1995，61，： | 1 |
| 4 | Scanning tunneling microscopy of individual molecules: beyond imaging显示文摘 | Gimzewski J K Jung T A Cuberes M T | 1997 | Surface Science1997，386，： | 1 |
| 5 | Electronics using hybrid-molecular and mono-molccular devices 显示文摘 | Joachim C Gimzewski J K Aviram A | 2000 | Nature2000，408，： | 1 |
| 6 | Nanoscale science of single molecules using local probes 显示文摘 | Gimzewski J K Joachim C | 1999 | Science1999，238，5408： | 1 |
| 7 | Electronics using hybrid - molecular and mono - molecular devices 显示文摘 | JOACHIM C GIMZEWSKI J K AVIRAM A | 2000 | Nature2000，408，6812： | 1 |
| 8 | Electronic Using Hybrid-molecular and Mono-molecular Device显示文摘 | Joachim C Gimzewski J K Aviram A | 2000 | Nature2000，408，： | 1 |
| 9 | Electronics using hybrid-molecular and mono-molecular devices 显示文摘 | Gimzewski J K Aviram A | 2000 | Nature2000，408，： | 1 |
| 10 | Nanoscale Science of Single Molecules Using Local Probes显示文摘 | Gimzewski J K Joachim C | 1999 | Science1999，283，： | 1 |
| 11 | Room temperature supramolecular repositioning at molecular interfaces using a scanning tunneling microscope 显示文摘 | Cuberes M T Schlittler R R Gimzewski J K | 1997 | Surface Science1997，371，： | 1 |
| 12 | Electronics using hybrid-molecular and mono-molecular devices显示文摘 | Joachim C Gimzewski J K Aviram A | 2000 | Nature2000，408，： | 1 |
| 13 | Scanning tunneling microscopy of individual molecules : be- yond imaging显示文摘 | Gimzewski J K Jung T A Cuberes M T | 1997 | Surface Science1997，386，13： | 1 |
| 14 | Nanoscale science of single molecules using local probes显示文摘 | Gimzewski J K Joachim C | 1999 | Science1999，283，： | 1 |
| 15 | Electronics using hybrid molecular and mono-molecular devices显示文摘 | JOACHIM C GIMZEWSKI J K AVIRAM A | 2000 | Nature2000，408，： | 1 |
| 16 | Electronics Using Hybrid-molecular and Mono-molecular Devices 显示文摘 | Joachim C Gimzewski J K Aviram A | 2000 | Nature2000，408，： | 1 |
| 17 | Nanoscale visualization and characterization of Myxococcus xanthus cell with atomic force microscopy显示文摘 | PELLING A E LI Y U SHI W Y GIMZEWSKI J K | 2005 | PNAS2005，102，18： | 1 |
| 18 | Scanning tunneling microscopy of individual molecules of coper phthalocyanine adsorbed on polycrystalline silver surface 显示文摘 | Gimzewski J K Stoll E Schlittler R R | 1987 | Surface Science1987，，： | 1 |
| 19 | Nanoscale visualization and characterization of Myxococcus xanthus cells with atomic force microscopy显示文摘 | Pelling AE Li Y Shi W Gimzewski JK | | 0，，： | 1 |
| 20 | Rotation of a single molecule within a supramolecular beating显示文摘 | Gimzewski J K Joachim C Schlittler R R | 1998 | Science1998，281，5376： | 1 |
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