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| 1 | Endoscopic submucosal dissection in dogs in a World Gastroenterology Organisation training center显示文摘AIM: To evaluate if canine models are appropriate for teaching endoscopy fellows the techniques of endoscopic submucosal dissection (ESD). METHODS: ESD was performed in 10 canine models under general anesthesia, on artificial lesions of the esophagus or stomach marked with coagulation points. After ESD, each canine model was euthanized and surgical resection of the esophagus or stomach was carried out according to 'The Principles of Humane Experimental Technique, Russel and Burch'. The ESD specimens were fixed with needles on cork submerged in a formol solution with the esophagus or stomach, and delivered to the pathology department to be analyzed. RESULTS: ESD was completed without complications using the Hook-knife in five esophageal areas, with a procedural duration of 124 ± 19 min, a length of 27.4 ± 2.6 mm and a width of 21 ± 2.4 mm. ESD was also completed without complications using the IT-knife2 in five gastric areas, with a procedural duration of 92.6 ± 19 min, a length of 32 ± 2.5 mm and a width of 18 ± 3.7 mm. CONCLUSION: ESD is feasible in the normal esopha- gus and stomach of canine models, which are appropriate for teaching this technique. | Miguel A Tanimoto Gonzalo Torres-Villalobos Rikiya Fujita Patricio Santillan-Doherty Jorge Albores-Saavedra German Gutierrez Luis A Martin-del-Campo Carlos Bravo-Reyna Octavio Villanueva Jose J Villalobos Misael Uribe Miguel A Valdovinos | 2010 | World Journal of Gastroenterology2010，16，14： | 7 |
| 2 | Effects of cellulase and xylanase enzymes mixed with increasing doses of Salix babylonica extract on in vitro rumen gas production kinetics of a mixture of corn silage with concentrate显示文摘An in vitro gas production(GP) technique was used to investigate the effects of combining different doses of Salix babylonica extract(SB) with exogenous fibrolytic enzymes(EZ) based on xylanase(X) and cellulase(C), or their mixture(XC; 1:1 v/v) on in vitro fermentation characteristics of a total mixed ration of corn silage and concentrate mixture(50:50, w/w) as substrate. Four levels of SB(0, 0.6, 1.2 and 1.8 m L g–1 dry matter(DM)) and four supplemental styles of EZ(1 μL g–1 DM; control(no enzymes), X, C and XC(1:1, v/v) were used in a 4×4 factorial arrangement. In vitro GP(m L g–1 DM) were recorded at 2, 4, 6, 8, 10, 12, 24, 36, 48 and 72 h of incubation. After 72 h, the incubation process was stopped and supernatant p H was determined, and then filtered to determine dry matter degradability(DMD). Fermentation parameters, such as the 24 h gas yield(GY24), in vitro organic matter digestibility(OMD), metabolizable energy(ME), short chain fatty acid concentrations(SCFA), and microbial crude protein production(MCP) were also estimated. Results indicated that there was a SB?EZ interaction(P<0.0001) for the asymptotic gas production(b), the rate of gas production(c), GP from 6 to 72 h, GP2(P=0.0095), and GP4(P=0.02). The SB and different combination of enzymes supplementation influenced(P<0.001) in vitro GP parameters after 12 h of incubation; the highest doses of SB(i.e., 1.8 m L g–1 DM), in the absence of any EZ, quadratically increased(P<0.05) the initial delay before GP begins(L) and GP at different incubation times, with lowering b(quadratic effect, P<0.0001) and c(quadratic effect, P<0.0001; linear effect, P=0.0018). The GP was the lowest(P<0.05) when the highest SB level was combined with cellulose. There were SB?EZ interactions(P<0.001) for OMD, ME, the partitioning factor at 72 h of incubation(PF72), GY24, SCFA, MCP(P=0.0143), and p H(P=0.0008). The OMD, ME, GY24 and SCFA with supp lementation of SB extract at 1.8 m L g–1 DM were higher(P<0.001) than the other treatments, however,PF72 was lower(quadratic effect, P=0.0194) than the other levels. Both C and X had no effect(P>0.05) on OMD, p H, ME, GY24, SCFA and MP. The combination of SB with EZ increased(P<0.001) OMD, ME, SCFA, PF72 and GP24, whereas there was no impact on p H. It could be concluded that addition of SB extract, C, and X effectively improved the in vitro rumen fermentation, and the combination of enzyme with SB extract at the level of 1.2 m L g–1 was more effective than the other treatments. | Abdelfattah Z M Salem German Buendía-Rodríguez Mona M M Elghandour María A Mariezcurrena Berasain Francisco J Pea Jiménez Alberto B Pliego Juan C V Chagoyán María A Cerrillo Miguel A Rodríguez | 2015 | Journal of Integrative Agriculture2015，14，1： | 4 |
| 3 | Solid-state contributions to densification during liquid-phase sintering显示文摘 | Johnson J L German R M | 1996 | Metal-lurgical Transaction B1996，8，1： | 1 |
| 4 | Protein aggregation, metals and oxidative stress in neurodegenerative diseases 显示文摘 | Tabner B J El Agnaf OM German M J | 2005 | Biochem Soc Trans2005，33，5： | 1 |
| 5 | Relative deficiency in IgA production by duodenal explants from german shepherd dogs with small intestinal Disease显示文摘 | GERMAN A J HALL E J DAYM J | 2000 | Vet Immunol Immunopatho2000，76，12： | 1 |
| 6 | A Collaborative Study to Develop a Standardized Food Protein Solubility Procedure 显示文摘 | MORt~ C V GERMAN B KINSELLA J E | 1985 | Journal of Food Science1985，50，： | 1 |
| 7 | Design and kinematic analysis of 3PSS - 1S wrist for needle insertion guidance 显示文摘 | Lisandro J Puglisi Roque J Saltaren German Rey Portoles | 2013 | Robotics and Autonomous Systems2013，61，5： | 1 |
| 8 | Effect of liquid content on distortion and rearrangement densification of liquid- phase-sintered W-Cu显示文摘 | Johnson J L Brezovsky J J German R M | 2005 | Metallurgical & Materials Transactions A2005，36，6： | 1 |
| 9 | Food processing and lipid oxidation显示文摘 | German J B | 1999 | Adv Exp Med Biol1999，459，： | 1 |
| 10 | Geochemistry of a hydrothermal sediment core from the OBS vent- field, 21°N East Pacific rise 显示文摘 | German C R Palmer M Edmond J M | 1999 | Chemical Geology1999，155，： | 1 |
| 11 | The health benefits of wine 显示文摘 | German J B Walzem R L | 2000 | Annu Rev Nutr2000，20，： | 1 |
| 12 | Accuracy Assessment and Validation of Classified Satellite Imagery of Texas Coastal Wetlands 显示文摘 | Hinson J M German C D Pulich W J | 1994 | Marine Technology Society Journal1994，，28： | 1 |
| 13 | Dynamometric analysis on the sinter-ingbehavior of nanocrystalline W-Cu Prepaerd by mehanieal al-loying显示文摘 | Johnson J L German R M | 2000 | Alloying and compounds2000，24，1： | 1 |
| 14 | 查看详情显示文摘 | Brouwer H D Schellekens M A J Klumperman B Monteiro M J German A L | | 0，，： | 1 |
| 15 | The health benefits of wine显示文摘 | German J B | 2000 | Annual Revi Nutr2000，20，： | 1 |
| 16 | An approach to fingerprint filter design 显示文摘 | O' German L Nickerson J V | 1989 | Pattern Recognition1989，22，1： | 1 |
| 17 | Mealybug wilt, mealybugs, and ants on pineapple显示文摘 | Rohrbach K G Beardsley J W German T L | 1988 | Plant Dis1988，72，： | 1 |
| 18 | Geochemistry of a hydrothermal sediment core from the OBS vent-field, 21°N East Pacific Rise 显示文摘 | German G R Hergt J Palmer M R Edmond J M | 1999 | Chem Geol1999，155，12： | 1 |
| 19 | Power Metallurgy Pro-cessing ofThermal Management Materials for Microelectronic Ap-plications显示文摘 | German R M Hens K F Johnson J L | 1994 | International Journal of Power Metallurgy1994，30，2： | 1 |
| 20 | Development of an annotatedlibrary of neutral human milk oligosaccharides 显示文摘 | WU S TAO N German J B et ai | 2010 | J ProteomeRes2010，9，41： | 1 |
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