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您的检索式：作者名="Geoffrey Collins"
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| 1 | FK506对实验性卒中疗效的系统评价和Meta分析显示文摘FK506是治疗急性卒中的一种候选用药。决定一种药物是否可以用于临床试验,应当以全面的、无偏倚的动物实验数据的评估为依据,同时还应考虑到这些数据的局限性。这种评估不但应包括药物疗效,而且也应包括药效在体内的特征和局限性。本研究应用系统评价和Meta分析的方法对FK506在卒中动物模型中保护作用的证据进行评价。总共纳入了29个描述了实验步骤的研究,包括1759只动物。结果显示,FK506疗效的点估计值(结局指标的改善)是31.3%[95%CI(0.272-0.354)]。在采用氯胺酮麻醉和短暂性脑缺血的动物实验中,FK506的疗效更高,在使用大鼠,合并其他疾病的动物及仅以梗死面积为疗效指标的实验中,FK506疗效较低。已发表的实验研究质量均接近临床试验标准,但在高质量的研究中,FK506的疗效较低。FK506在实验性脑卒中的研究中,虽然显示出有明显的疗效,但是应注意由于研究质量和可能的发表偏倚等冈素的影响,FK506的疗效可能被过高估计。 | Malcolm R Macleod Tori O’Collins Laura L Horky David W Howells Geoffrey A Donnan 段建钢 | 2006 | 中国循证医学杂志2006，6，6： | 3 |
| 2 | Method for hull-less barley transformation and manipulation of grain mixed-linkage beta-glucan显示文摘Hull-less barley is increasingly offering scope for breeding grains with improved characteristics for human nutrition; however, recalcitrance of hull-less cultivars to transformation has limited the use of these varieties. To overcome this limitation, we sought to develop an effective transformation system for hull-less barley using the cultivar Torrens. Torrens yielded a transformation efficiency of 1.8%,using a modified Agrobacterium transformation method.This method was used to over-express genes encoding synthases for the important dietary fiber component,(1,3;1,4)-b-glucan(mixed-linkage glucan), primarily present in starchy endosperm cell walls. Over-expression of the Hv Csl F6 gene, driven by an endosperm-specific promoter,produced lines where mixed-linkage glucan content increased on average by 45%, peaking at 70% in some lines,with smaller increases in transgenic Hv Csl H1 grain. Transgenic Hv Csl F6 lines displayed alterations where grain had a darker color, were more easily crushed than wild type and were smaller. This was associated with an enlarged cavity in the central endosperm and changes in cell morphology,including aleurone and sub-aleurone cells. This work provides proof-of-concept evidence that mixed-linkage glucan content in hull-less barley grain can be increased by over-expression of the Hv Csl F6 gene, but also indicates that hull-less cultivars may be more sensitive to attempts to modify cell wall composition. | Wai Li Lim Helen M. Collins Rohan R. Singh Natalie A. J. Kibble Kuok Yap Jillian Taylor Geoffrey B. Fincher Rachel A. Burton | 2018 | Journal of Integrative Plant Biology2018，60，5： | 1 |
| 3 | Transanal minimally invasive surgery to rescue anastomosis following leak after low anterior resection: A case report显示文摘Restorative low anterior resection(LAR)for rectal cancer carries a significant risk of anastomotic leak:One of the most feared complications in colorectal surgery.Operative management may include takedown of the anastomosis and end colostomy which,in some cases,is permanent.Other contemporary operative measures include over the scope clips and Endosponge.Recently,there have been case reports and a Society of American Endoscopic and Gastrointestinal Surgeons video on the novel use of transanal minimally invasive surgery(TAMIS)in the management of anastomotic leak.We present a 59-year-old female who underwent LAR after declining radiotherapy for a bulky 9 cm rectal tumour 9-10 cm from the anal verge.Following clinical deterioration,computed tomography demonstrated an anastomotic leak communicating with a 5-cm pelvic collection containing gas.At laparoscopy,pus and faeculent material were washed from the pelvic cavity and drains were placed.Intra-operative endoscopy demonstrated a 7-8 mm dehiscence at the anastomosis.The defect(approximately 7 cm from the anal verge)was successfully closed using TAMIS and a running V-lock suture.The patient recovered well and was discharged home on post-operative day 20.In this case,a low colorectal anastomotic leak was successfully rescued with TAMIS.This novel technique may be useful in the armamentarium of colorectal surgeons experienced in TAMIS. | James Wei Tatt Toh Henry Wang Geoffrey Collins Chelsea Beinke Elissa Zhang Alistair Escott Toufic El-Khoury Nimalan Pathma-Nathan | 2021 | Laparoscopic, Endoscopic and Robotic Surgery2021，4，4： | 0 |
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