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| 1 | 3-D打印:一种个性化制备复杂支架和组织工程植入物的多功能快速成型技术(英文)显示文摘目的对3-D打印技术构建的个性化生物支架和组织工程结构物的近期研究进展进行综述。方法广泛查阅近期采用3-D打印技术制备个性化生物支架和组织工程结构物的相关文献,并对作者课题组采用3-D打印技术用于组织工程领域的近期研究工作进行总结。结果相较于仅适用于特定生物材料的快速成型法,3-D打印技术适用于几乎所有膏状生物材料,为构建生物支架、多相支架甚至是包含活细胞的组织工程结构物提供了更多的可能和选择。结论 3-D打印技术是一种越来越重要的快速成型技术,该技术保证了生物材料的广泛选择应用,并使得直接打印敏感生物物质(如生长因子和细胞)成为可能。 | 罗永祥 Ashwini Rahul Akkineni Anja Lode Michael Gelinsky | 2014 | 中国修复重建外科杂志2014，28，3： | 10 |
| 2 | Dynamic poroelasticity of thinly layered structures显示文摘 | Gelinsky S Shapiro S A Muller T | 1998 | International Journal Solids and Structures1998，35，34： | 1 |
| 3 | Porelatic Backus-averaging for anisotropic layered fluid and gas saturated sediments显示文摘 | Gelinsky S Shapiro S A | 1997 | Geophysics1997，62，6： | 1 |
| 4 | Use of amineratized collagen membrane to enhance repair of calvarial defects in rats 显示文摘 | Schliephake H Tavassol F Gelinsky M | 2004 | Clin Oral Impl Res2004，2004，： | 1 |
| 5 | 3D printing of patient-specific implants for osteochondral defects: workflow for an MRI-guided zonal design显示文摘Magnetic resonance imaging(MRI)is a common clinical practice to visualize defects and to distinguish different tissue types and pathologies in the human body.So far,MRI data have not been used to model and generate a patient-specific design of multilayered tissue substitutes in the case of interfacial defects.For orthopedic cases that require highly individual surgical treatment,implant fabrication by additive manufacturing holds great potential.Extrusion-based techniques like 3D plot-ting allow the spatially defined application of several materials,as well as implementation of bioprinting strategies.With the example of a typical multi-zonal osteochondral defect in an osteochondritis dissecans(OCD)patient,this study aimed to close the technological gap between MRI analysis and the additive manufacturing process of an implant based on dif-ferent biomaterial inks.A workflow was developed which covers the processing steps of MRI-based defect identification,segmentation,modeling,implant design adjustment,and implant generation.A model implant was fabricated based on two biomaterial inks with clinically relevant properties that would allow for bioprinting,the direct embedding of a patient’s own cells in the printing process.As demonstrated by the geometric compatibility of the designed and fabricated model implant in a stereolithography(SLA)model of lesioned femoral condyles,a novel versatile CAD/CAM workflow was successfully established that opens up new perspectives for the treatment of multi-zonal(osteochondral)defects. | David Kilian Philipp Sembdner Henriette Bretschneider Tilman Ahlfeld Lydia Mika Jörg Lützner Stefan Holtzhausen Anja Lode Ralph Stelzer Michael Gelinsky | 2021 | Bio-Design and Manufacturing2021，4，4： | 1 |
| 6 | Zinc complex- ation of glutathione and glutathione-derived peptides 显示文摘 | Gelinsky M Vog|er R Vahrenkamp H | 2003 | Inorganica Chimica Acta2003，334，： | 1 |
| 7 | A novel and easy-to-prepare strontium(II) modified calcium phosphate bone cement with enhanced mechanical properties显示文摘 | M. Schumache< !--Q1: Please confirm that given names and surnames have been identified correctly.--> r A. Hen? M. Rohnke M. Gelinsky | 2013 | Acta Biomaterialia2013，，7： | 1 |
| 8 | Zinc complexation ofglutathione and glutathione-derived peptides显示文摘 | Gelinsky M Vogler R Vahrenkamp H | 2003 | Inorganicachimica acta2003，344，： | 1 |
| 9 | Zinc complexation of glutathione and glutathione-derived peptides显示文摘 | M Gelinsky R Vogler H Vahrenkamp | 2003 | Inorganica Chimica Acta2003，334，： | 1 |
| 10 | Solution behacvior and zinc complexation of di and tripeptides with two cysteine units显示文摘 | VOGLER R GELINSKY M GUO L F | 2002 | Inorganica Chimica Acta2002，339，： | 1 |
| 11 | Ectopic bone formation in nude rats using human osteoblasts seeded poly(3) hydroxybutyrate embroidery and hydroxyapatite-col agen tapes constructs显示文摘 | Mai R Hagedorn MG Gelinsky M | | 0，，2： | 1 |
| 12 | Swelling and mechanical properties of alginate hydrogels with respect to promotion of neural growth 显示文摘 | Matyash M Despang F Ikonomidou C Gelinsky M | 2014 | Tissue Engineering: Part C2014，20，5： | 1 |
| 13 | Molecular and phenotypic characterization of Lactobacillus curvatus isolated from handmade Brazilian salami显示文摘 | Baratto C M Gelinski J Debastiani J | 2012 | African Journal of Biotechnology2012，11，11： | 1 |
| 14 | Cultivation of human bone marrow stromal cells on three-dimensional scaffolds of mineralized collagen:influence of seeding density on colonization,proliferation and osteogenic differentiation显示文摘 | Lode A Bernhardt A Gelinsky M | | 0，，7： | 1 |
| 15 | Zinc complexation of glutathione and glutathione-derived peptides显示文摘 | Gelinsky M Vogler R Vahrenkamp H | 2003 | Inorganica chimica acta2003，344，： | 1 |
| 16 | Biomimetic porous scaffolds with high elasticity made from mineralized collagen-Animal study显示文摘 | Yokoyama A Gelinsky M Kawasaki T | 2005 | J Biomed Mater Res B Appl Biomater2005，75，2： | 1 |
| 17 | 硅酸钙、β-磷酸三钙和羟基磷灰石三相陶瓷复合物与骨细胞活性的体外鉴定(英文)显示文摘由于羟基磷灰石陶瓷与骨无机质的化学成份相似,所以10年来研究者对羟基磷灰石陶瓷在骨修复替换中的应用进行了深入的研究。羟基磷灰石因其高生物相容性和骨传导性已被广泛的应用,但羟基磷灰石的再吸收能力差。文章对由硅酸钙、β-磷酸三钙和羟基磷灰石组成的三相陶瓷复合物进行了研究。在三相陶瓷复合物环境下,采用不同比率硅酸钙、β-磷酸三钙和羟基磷灰石对骨髓夹源间充质干细胞进行体外研究。细胞在加入和没有加入成骨细胞的情况下培养28d。采用碱性磷酸酶活性、碱性磷酸酶基因码转录和骨唾液蛋白Ⅱ表达评估成骨分化水平。成骨细胞和破骨细胞在陶瓷复合80%羟基磷灰石材料上完成预实验。将鼠颅盖骨成骨细胞与人单核细胞共同培养以分析其向破骨细胞分化的可能性。结果显示人骨髓间充质干细胞在陶瓷复合物材料上表现出快速黏附和高度增殖率,在所有实验材料上,成骨诱导的骨髓间充质干细胞碱性磷酸酶活性增加,碱性磷酸酶基因码转录和骨唾液酸蛋白Ⅱ的表达在3种材料上同样增加。扫描电镜和聚焦激光扫描显微镜结果证明在陶瓷复合80%羟基磷灰石材料上的人单核细胞向破骨样细胞分化。结果提示制备的三相陶瓷复合材料支持人骨髓间充质干细胞的黏附、增殖和成骨分化,以及人单核细胞与成骨细胞联合培养条件下破骨细胞形成。 | Anne Bernhardt Anja Lode Sivadasan Suresh Babu Antje Vogel Thomas Hanke Sebastian Thieme Hare Krishna Varma Angela Rosen-Wolff Michael Gelinsky Annie John | 2010 | 中国组织工程研究与临床康复2010，14，16： | 0 |
| 18 | Treatment of critical bone defects using calcium phosphate cement and mesoporous bioactive glass providing spatiotemporal drug delivery显示文摘Calcium phosphate cements (CPC) are currently widely used bone replacement materials with excellent bioactivity, but have considerable disadvantages like slow degradation. For critical-sized defects, however, an improved degradation is essential to match the tissue regeneration, especially in younger patients who are still growing. We demonstrate that a combination of CPC with mesoporous bioactive glass (MBG) particles led to an enhanced degradation in vitro and in a critical alveolar cleft defect in rats. Additionally, to support new bone formation the MBG was functionalized with hypoxia conditioned medium (HCM) derived from rat bone marrow stromal cells. HCM-functionalized scaffolds showed an improved cell proliferation and the highest formation of new bone volume. This highly flexible material system together with the drug delivery capacity is adaptable to patient specific needs and has great potential for clinical translation. | Richard Frank Richter Corina Vater Margarete Korn Tilman Ahlfeld Martina Rauner Winnie Pradel Bernd Stadlinger Michael Gelinsky Anja Lode Paula Korn | 2023 | Bioactive Materials2023，，10： | 0 |
| 19 | Evaluation of different crosslinking methods in altering the properties of extrusion-printed chitosan-basedmulti-material hydrogel composites显示文摘Three-dimensional printing technologies exhibit tremendous potential in the advancing fields of tissue engineering and regenerative medicine due to the precise spatial control over depositing the biomaterial.Despite their widespread utilization and numerous advantages,the development of suitable novel biomaterials for extrusion-based 3D printing of scaffolds that support cell attachment,proliferation,and vascularization remains a challenge.Multi-material composite hydrogels present incredible potential in this field.Thus,in this work,a multi-material composite hydrogel with a promising formulation of chitosan/gelatin functionalized with egg white was developed,which provides good printability and shape fidelity.In addition,a series of comparative analyses of different crosslinking agents and processes based on tripolyphosphate(TPP),genipin(GP),and glutaraldehyde(GTA)were investigated and compared to select the ideal crosslinking strategy to enhance the physicochemical and biological properties of the fabricated scaffolds.All of the results indicate that the composite hydrogel and the resulting scaffolds utilizing TPP crosslinking have great potential in tissue engineering,especially for supporting neo-vessel growth into the scaffold and promoting angiogenesis within engineered tissues. | Suihong Liu Haiguang Zhang Tilman Ahlfeld David Kilian Yakui Liu Michael Gelinsky Qingxi Hu | 2023 | Bio-Design and Manufacturing2023，6，2： | 0 |
| 20 | 3D Printing of Cell-Container-Like Scaffolds for Multicell Tissue Engineering显示文摘The development of an engineered non-contact multicellular coculture model that can mimic the in v iv o cell microenvironment of human tissues remains challenging.In this study,we successfully fabricated a cell-container-like scaffold composed of p-tricalcium phosphate/hydroxyapatite(p-TCP/HA)bioceramic that contains four different pore structures,including triangles,squares,parallelograms,and rectangles,by means of three-dimensional(3D)printing technology.These scaffolds can be used to simultaneously culture four types of cells in a non-contact way.An engineered 3D coculture model composed of human bone-marrow-derived mesenchymal stem cells(HBMSCs),human umbilical vein endothelial cells(HUVECs),human umbilical vein smooth muscle cells(HUVSMCs),and human dermal fibroblasts(HDFs)with a spatially controlled distribution was constructed to investigate the individual or synergistic effects of these cells in osteogenesis and angiogenesis.The results showed that three or four kinds of cells cocultured in 3D cell containers exhibited a higher cell proliferation rate in comparison with that of a single cell type.Detailed studies into the cell-cell interactions between HBMSCs and HUVECs revealed that the 3D cell containers with four separate spatial structures enhanced the angiogenesis and osteogenesis of cells by amplifying the paracrine effect of the cocultured cells.Furthermore,the establishment of multicellular non-contact systems including three types of cells and four types of cells,respectively,cocultured in 3D cell containers demonstrated obvious advantages in enhancing osteogenic and angiogenic differentiation in comparison with monoculture modes and two-cell coculture modes.This study offers a new direction for developing a scaffold-based multicellular non-contact coculture system for tissue regeneration. | Xiaoya Wang Meng Zhang Jingge Ma Mengchi Xu Jiang Chang Michael Gelinsky Chengtie Wu | 2020 | Engineering2020，6，11： | 0 |
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