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| 1 | Underexpression of LATS1 TSG in colorectal cancer is associated with promoter hypermethylation显示文摘AIM:To investigate large tumor suppressor 1 (LATS1 ) expression, promoter hypermethylation, and microsatellite instability in colorectal cancer (CRC).METHODS:RNA was isolated from tumor tissue of 142 CRC patients and 40 colon mucosal biopsies of healthy controls. After reverse transcription, quantitative polymerase chain reaction (PCR) was performed, and LATS1 expression was normalized to expression of the ACTB and RPL32 housekeeping genes. To analyze hypermethylation, genomic DNA was isolated from 44 tumor CRC biopsies, and methylation-specific PCR was performed. Microsatellite instability (MSI) status was checked with PCR using BAT26, BAT25, and BAT40 markers in the genomic DNA of 84 CRC patients, followed by denaturing gel electrophoresis. RESULTS:Decreased LATS1 expression was found in 127/142 (89.4%) CRC cases with the average ratio of the LATS1 level 10.33 ± 32.64 in CRC patients vs 32.85 ± 33.56 in healthy controls. The lowest expression was found in Dukes' B stage tumors and G1 (welldifferentiated) cells. Hypermethylation of the LATS1 promoter was present in 25/44 (57%) CRC cases analyzed. LATS1 promoter hypermethylation was strongly associated with decreased gene expression; methylated cases showed 162× lower expression of LATS1 than unmethylated cases. Although high-grade MSI (mutation in all three markers) was found in 14/84 (17%) cases and low-grade MSI (mutation in 1-2 markers) was found in 30/84 (36%) cases, we found no association with LATS1 expression. CONCLUSION:Decreased expression of LATS1 in CRC was associated with promoter hypermethylation, but not MSI status. Such reduced expression may promote progression of CRC. | Piotr M Wierzbicki Krystian Adrych Dorota Kartanowicz Marcin Stanislawowski Anna Kowalczyk Janusz Godlewski Iwona Skwierz-Bogdanska Krzysztof Celinski Tomasz Gach Jan Kulig Bartlomiej Korybalski Zbigniew Kmiec | 2013 | World Journal of Gastroenterology2013，19，27： | 8 |
| 2 | Early appearance of activated matrix metalloproteinase-9 and blood brain bartier distruption in mice after focal cerebral ischemia and reperfusion 显示文摘 | Fujimura M Gache Y Fujimura M Y | 1999 | Brain Res1999，842，1： | 1 |
| 3 | Frequency of sutoinue disease in 218 patients with autoimmune thyroid pathologies显示文摘 | Gaches F Delaire L Nadalon S | 1998 | Rev Med Intern1998，19，3： | 1 |
| 4 | Cerebral edema follow-ing photodynamic therapy using endogenous and exogenous photosensitizers in normal brain 显示文摘 | Mathews MS Chighvinadze D Gach HM | 2011 | Lasers Sury Med2011，43，9： | 1 |
| 5 | The role of morphine in regulation of cancer cell growth显示文摘 | Gach K Wyr(e)bska A Fichna J | | 0，，03： | 1 |
| 6 | Long-term prognostic significance of high-sensitivity C-reactive protein before and after coronary angioplasty in patients with stable angina pectoris显示文摘 | Gach O Legrand V Biessaux Y | 2007 | Am J Cardiol2007，99，1： | 1 |
| 7 | Photodistributed lichenoid drug eruption seeondary to solifenacin显示文摘 | Shalders K Gach JE | 2008 | Clin Exp Dermatol2008，33，3： | 1 |
| 8 | Long-term prognostic significance of high-sensitivity C-reactive protein before and after coronary angioplasty in patients with stable angina pectoris显示文摘 | Gach O Legrand V Biessaux Y | 2007 | Am J Cardiol2007，99，1： | 1 |
| 9 | Purification and characterization of the sea urchin embryo hatching enzyme 显示文摘 | LEPAGE T GACHE C | 1989 | J Biological Chem1989，264，9： | 1 |
| 10 | Two families with Grei- ther's syndrome caused by keratin 1 mutation 显示文摘 | Gach JE Munro CS Lane EB | 2005 | J Am Acad Dermatol2005，53，51： | 1 |
| 11 | Purification and characterization of the sea urchin embryo hatching enzyme 显示文摘 | Lepage T Gache C | 1989 | J Biol Chem1989，264，9： | 1 |
| 12 | Early expression of a collagenase-like hatching enzyme gene in the sea urchin embryo 显示文摘 | Lepage T Gache C | 1990 | Embo J1990，9，9： | 1 |
| 13 | Total dose effects on negative voltage regulator 显示文摘 | BEACOUR J T CARRIERE T GACH A | 1994 | IEEE Trans Nucl Sci1994，41，6： | 1 |
| 14 | The role of selective internal urethrotomy in the management of u- rethral stricture: a multi-centre evaluation 显示文摘 | Gaches C G Ashken M H Dunn M | 1979 | Br J Urol1979，51，6： | 1 |
| 15 | Catalytic reduction of hexachlorobenzene and pentachlorobenzene by cobalt(Ⅰ) salen electrogenerated at vitreous carbon cathodes in dimethylformamide显示文摘 | Philip C Gach Jonathan A Karty Dennis G Peters | 2008 | Electroanalytical Chemistry2008，，612： | 1 |
| 16 | Sulfamethoxypyridazine-responsive pemphigoid nodularis:a report of two cases显示文摘 | Gach JE Wilson NJ Wojnarowska F | 2005 | J Am Acad Dermatol2005，53，21： | 1 |
| 17 | Sulfam ethoxypyr i-dazine-responsive pemphigoid nodularis: a report of two cases显示文摘 | Gach JE Wilson NJ Wojnarowska F | 2005 | J Am Acad Dermatol2005，53，2： | 1 |
| 18 | HACD1,a regulator of membrane composition and fluidity,promotes myoblast fusion and skeletal muscle growth显示文摘The reduced diameter of skeletal myofibres is a hallmark of several congenital myopathies,yet the underlying cellular and molecular mechanisms remain elusive.In this study,we investigate the role of HACD1/PTPLA,which is involved in the elongation of the very long chain fatty acids,in muscle fibre formation.In humans and dogs,HACD1 deficiency leads to a congenital myopathy with fibre size disproportion associated with a generalized muscleweakness.Throughanalysis of HACD1-deficient Labradors,Hacd1-knockout mice,and Hacd1-deficient myoblasts,we provide evidence that HACD1 promotes myoblast fusion during muscle development and regeneration.We further demonstrate that in normal differentiating myoblasts,expression of the catalytically active HACD1 isoform,which is encoded by a muscle-enriched splice variant,yields decreased lysophosphatidylcholine content,a potent inhibitor of myoblast fusion,and increased concentrations of≥C18 and monounsaturated fatty acids of phospholipids.These lipid modifications correlate with a reduction in plasma membrane rigidity.In conclusion,we propose that fusion impairment constitutes a novel,non-exclusive pathological mechanism operating in congenital myopathies and reveal that HACD1 is a key regulator of a lipid-dependent muscle fibre growth mechanism. | Jordan Blondelle Yusuke Ohno Vincent Gache Stephane Guyot Sebastien Storck Nicolas Blanchard-Gutton Ines Barthelemy Gemma Walmsley Anaelle Rahier Stephanie Gadin Marie Maurer Laurent Guillaud Alexandre Prola Arnaud Ferry Genevieve Aubin-Houzelstein Jean Demarquoy Frederic Relaix Richard JPiercy Stephane Blot Akio Kihara Laurent Tiret Fanny Pilot-Storck | 2015 | Journal of Molecular Cell Biology2015，7，5： | 1 |
| 19 | The influence of opioids on matrix metalloproteinase-2 and -9 secretion and mRNA levels in MCF-7 breast cancer lines显示文摘 | Gach K Szemraj J Wyrebska A | 2011 | Mol Biol Rep2011，38，2： | 1 |
| 20 | Functional characterization of opioid receptor ligands by aequorin luminescence-based calcium assay显示文摘 | Fichna J Gach K Piestrzeniewicz M | 2006 | J Pharmacol Exp Ther2006，317，3： | 1 |
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