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3篇 您的检索式:作者名="Gabriel Castrillo"
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1Arsenite provides a selective signal that coordinates arsenate uptake and detoxification through the regulation of PHR1 stability in Arabidopsis显示文摘In nature,plants acquire nutrients from soils to sustain growth,and at the same time,they need to avoid the uptake of toxic compounds and/or possess tolerance systems to cope with them.This is particularly challenging when the toxic compound and the nutrient are chemically similar,as in the case of phosphate and arsenate.In this study,we demonstrated that regulatory elements of the phosphate starvation response(PSR)coordinate the arsenate detoxification machinery in the cell.We showed that arsenate repression of the phosphate transporter PHT1;1 is associated with the degradation of the PSR master regulator PHR1.Once arsenic is sequestered into the vacuole,PHR1 stability is restored and PHT1;1 expression is recovered.Furthermore,we identified an arsenite responsive SKP1-like protein and a PHR1 interactor F-box(PHIF1)as constituents of the SCF complex responsible for PHR1 degradation.We found that arsenite,the form to which arsenate is reduced for compartmentalization in vacuoles,represses PHT1;1 expression,providing a highly selective signal versus phosphate to control PHT1;1 expression in response to arsenate.Collectively,our results provide molecular insights into a sensing mechanism that regulates arsenate/phosphate uptake depending on the plant’s detoxification capacity.Cristina Navarro Cristian Mateo-Elizalde Thotegowdanapalya C.Mohan Eduardo Sanchez-Bermejo Oscar Urrutia Maria Nieves Fernandez-Muniz Jose M.Garcia-Mina Riansares Munoz Javier Paz-Ares Gabriel Castrillo Antonio Leyva 2021Molecular Plant2021,14,9:3
2Arsenite provides a selective signal that coordinates arsenate uptake and detoxification through the regulation of PHR1 stability in Arabidopsis显示文摘(Molecular Plant 14,1489-1507;September 6,2021)In the“Accession numbers”section in the originally published version of our manuscript,we mistakenly listed At5g44570 as the Arabidopsis Genome Initiative locus identifier for the gene CAD1.The correct locus identifier is At5g44070.We apologize for any confusion this has caused.Cristina Navarro Cristian Mateo-Elizalde Thotegowdanapalya CMohan Eduardo Sánchez-Bermejo Oscar Urrutia María Nieves Fernández-Muñiz JoséMGarcía-Mina Riansares Muñoz Javier Paz-Ares Gabriel Castrillo Antonio Leyva 2022Molecular Plant2022,15,11:0
3Temperature changes in the root ecosystem affect plant functionality显示文摘Climate change is increasing the frequency of extreme heat events that aggravate its negative impact on plant development and agricultural yield.Most experiments designed to study plant adaption to heat stress apply homogeneous high temperatures to both shoot and root.However,this treatment does not mimic the conditions in natural fields,where roots grow in a dark environment with a descending temperature gradient.Excessively high temperatures severely decrease cell division in the root meristem,compromising root growth,while increasing the division of quiescent center cells,likely in an attempt to maintain the stem cell niche under such harsh conditions.Here,we engineered the TGRooZ,a device that generates a temperature gradient for in vitro or greenhouse growth assays.The root systems of plants exposed to high shoot temperatures but cultivated in theTGRooZ grow efficiently and maintain their functionality to sustain proper shoot growth and development.Furthermore,gene expression and rhizosphere or root microbiomecomposition are significantly less affected in TGRooZ-grown roots than in high-temperature-grown roots,correlating with higher root functionality.Our data indicate that use of the TGRooZ in heat-stress studies can improve our knowledge of plant response to high temperatures,demonstrating its applicability from laboratory studies to the field.Mary Paz González-García Carlos M.Conesa Alberto Lozano-Enguita Victoria Baca-González Bárbara Simancas Sara Navarro-Neila María Sánchez-Bermúdez Isai Salas-González Elena Caro Gabriel Castrillo Juan C.del Pozo 2023Plant Communications2023,4,3:0
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