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| 1 | Long-term and non-invasive in vivo tracking of DiD dye-labeled human hepatic progenitors in chronic liver disease models显示文摘BACKGROUND Chronic liver diseases(CLD)are the major public health burden due to the continuous increasing rate of global morbidity and mortality.The inherent limitations of organ transplantation have led to the development of stem cell-based therapy as a supportive and promising therapeutic option.However,identifying the fate of transplanted cells in vivo represents a crucial obstacle.AIM To evaluate the potential applicability of DiD dye as a cell labeling agent for longterm,and non-invasive in vivo tracking of transplanted cells in the liver.METHODS Magnetically sorted,epithelial cell adhesion molecule positive(1×106 cells/mL)fetal hepatic progenitor cells were labeled with DiD dye and transplanted into the livers of CLD-severe combined immunodeficiency(SCID)mice.Near-infrared(NIR)imaging was performed for in vivo tracking of the DiD-labeled transplanted cells along with colocalization of hepatic markers for up to 80 d.The existence of human cells within mouse livers was identified using Alu polymerase chain reaction and sequencing.RESULTS NIR fluorescence imaging of CLD-SCID mice showed a positive fluorescence signal of DiD at days 7,15,30,45,60,and 80 post-transplantation.Furthermore,positive staining of cytokeratin,c-Met,and albumin colocalizing with DiD fluorescence clearly demonstrated that the fluorescent signal of hepatic markers emerged from the DiD-labeled transplanted cells.Recovery of liver function was also observed with serum levels of glutamic-oxaloacetic transaminase,glutamate-pyruvate transaminase,and bilirubin.The detection of human-specific Alu sequence from the transplanted mouse livers provided evidence for the survival of transplanted cells at day 80.CONCLUSION DiD-labeling is promising for long-term and non-invasive in vivo cell tracking,and understanding the regenerative mechanisms incurred by the transplanted cells. | Chaturvedula Tripura Srinivas Gunda Sandeep Kumar Vishwakarma Avinash Raj Thatipalli Jedy Jose Mahesh Kumar Jerald Aleem Ahmed Khan Gopal Pande | 2022 | World Journal of Hepatology2022，14，10： | 1 |
| 2 | Cloning and functional identification of slc5a12 as a sodium-coupled low- affinity transporter for monocarboxylates (SMCF2) 显示文摘 | Srinivas SR Gopal E Zhuang L | 2005 | Biochem J2005，392，3： | 1 |
| 3 | Allicin (from garlic) induces caspase-apoptosis in cancer cells显示文摘 | Suby Oommen Ruby John Anto Gopal Srinivas | 2004 | European Journal of Pharmacology2004，485，1： | 1 |
| 4 | Al-licin(from garlic) induces caspase-apoptosis in cancer cells显示文摘 | SUBY OOMMEN RUBY JOHN ANTO GOPAL SRINIVAS | 2004 | European Journal of Pharmacology2004，485，1： | 1 |
| 5 | Vapor-phase alkylation of indole with methanol over zeolites显示文摘 | Gopal Venu D Srinivas B Durgakumari etc | 2002 | Applied Catalysis A: General2002，，224： | 1 |
| 6 | Carotid arterial occlusion in identical twins with Moyamoya显示文摘 | Srinivas K Narayanan M Gopal S | 1979 | Arch Neurol1979，36，4： | 1 |
| 7 | Al - licin ( from garlic) induces caspase - apoptosis in cancer cells 显示文摘 | ]Suby Oommen Ruby John Anto Gopal Srinivas | 2004 | European Joumal of Pharmacology2004，485，1： | 1 |
| 8 | Allicin(from garlic) induces caspase-mediated apoptosis in cancer cells显示文摘 | Oommen Suby Anto Ruby John Srinivas Gopal | 2004 | European Journal of Pharmacology2004，485，13： | 1 |
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