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| 1 | Probiotic metabolites from Bacillus coagulans GanedenBC30^(TM) support maturation of antigen-presenting cells in vitro显示文摘AIM:To study the effects of probiotic metabolites on maturation stage of antigen-presenting immune cells.METHODS:Ganeden Bacillus coagulans 30(GBC30) bacterial cultures in log phase were used to isolate the secreted metabolite(MET) fraction.A second fraction was made to generate a crude cell-wall-enriched fraction,by centrifugation and lysis,followed by washing.A preparation of MET was subjected to size exclusion centrifugation,generating three fractions:< 3 kDa,3-30 kDa,and 30-200 kDa and activities were tested in comparison to crude MET and cell wall in primary cultures of human peripheral blood mononuclear cell(PBMC) as a source of antigen-presenting mononuclear phagocytes.The maturation status of mononuclear phagocytes was evaluated by staining with monoclonal antibodies towards CD14,CD16,CD80 and CD86 and analyzed by flow cytometry.RESULTS:Treatment of PBMC with MET supported maturation of mononuclear phagocytes toward both macrophage and dendritic cell phenotypes.The biological activity unique to the metabolites included a reduction of CD14+ CD16+ pro-inflammatory cells,and this property was associated with the high molecular weight metabolite fraction.Changes were also seen for the dendritic cell maturation markers CD80 and CD86.On CD14dim cells,an increase in both CD80 and CD86 expression was seen,in contrast to a selective increase in CD86 expression on CD14bright cells.The co-expression of CD80 and CD86 indicates effective antigen presentation to T cells and support of T helper cell differentiation.The selective expression of CD86 in the absence of CD80 points to a role in generating T regulatory cells.CONCLUSION:The data show that a primary mechanism of action of GBC30 metabolites involves support of more mature phenotypes of antigen-presenting cells,important for immunological decision-making. | Kathleen F Benson Kimberlee A Redman Steve G Carter David Keller Sean Farmer John R Endres Gitte S Jensen | 2012 | World Journal of Gastroenterology2012，18，16： | 8 |
| 2 | Oil bodies and their associated proteins,oleosin and caleosin显示文摘 | Gitte I F John M Jason T C T | 2001 | Physiologia Plantarum2001，112，3： | 1 |
| 3 | Carcinogenesis in ureterosigmoidostomy显示文摘 | Gittes R F | 1986 | Urol Clin North Am1986，13，2： | 1 |
| 4 | 查看详情显示文摘 | Allersma M W Gittes F deCastro M J Stewart R J Schmidt C F | | 0，，： | 1 |
| 5 | Signals and noise in mieromeehanical measurements 显示文摘 | GITTES F SCHMIDT C | 1997 | Methods in Cell Biology1997，55，： | 1 |
| 6 | Interference model for back-focal- plane displacement detection in optical tweezers显示文摘 | GITTES F SCHMIDT C | 1998 | Optics Letters1998，23，1： | 1 |
| 7 | Family - centered care of children in hospital aconcept analysis显示文摘 | Gitte M Kirsten F | 2010 | Journal of Advanced Nursing2010，11，： | 1 |
| 8 | Benign cortical rest: a “pseudotumor” of the kidney显示文摘 | Filynn V J Gittes R F | 1972 | J Urol1972，108，7： | 1 |
| 9 | The role of germinal epithelium and spermatogenesis in the privileged survival of intratesticular grafts显示文摘 | Whitmore W F III Karsh L Gittes R F | 1985 | J Urol1985，134，： | 1 |
| 10 | The physiologic basis ofthe TUR syndrome显示文摘 | Bernstein G T Loughlin K R Gittes R F | 1989 | J Surg Res1989，46，2： | 1 |
| 11 | A strong sequence homology exists between the major RNA polymerase σfactor of Bacillus subtilis and Escgerichia coli显示文摘 | Gitt M G Wang L F Doi R H | 1985 | J Biol Chem1985，260，12： | 1 |
| 12 | Female Prostatitis显示文摘 | Ruben F Gittes | 2002 | Urol Clin N Am2002，29，3： | 1 |
| 13 | Flexural rigidity of microtubules and actin filaments measured from thermal fluctuations shape显示文摘 | Gittes F Mickey B Nettelton J | 1993 | J Cell Biol1993，120，： | 1 |
| 14 | Gender differences in nighttime plasma arginine vasopressin and delayed compensatory urine output in the elderly population after desmopressin 显示文摘 | Gitte MH Joegen F Soren N | 2007 | J Urol2007，178，6： | 1 |
| 15 | Flexural rigidity of microtubules and actin fila- ments measured from thermal fluctuations in shape显示文摘 | Gittes F Mickey B Nettleton J Howard J | 1993 | Journal of Cell Biology1993，120，4： | 1 |
| 16 | Renal brush biop sy: survey of indications, techniques and results 显示文摘 | Blute R D Gittes R R Gittes R F | 1981 | J Urol1981，126，： | 1 |
| 17 | Galectin-4 and galectin 6 are two closely related lectins expressed in mouse gastroin testinal tract 显示文摘 | Colnot C Poirier F | 1998 | J Biol Chem1998，273，5： | 1 |
| 18 | Urethral re- construction following an unsuccessful one-stage hypospadi- as repair 显示文摘 | Duckett J W Gittes R F Perlmutter A D | 1987 | World Journal of Urology1987，5，1： | 1 |
| 19 | No-incision pubovaginal suspension for stress incontinence显示文摘 | Gittes R F Loughlin K R | 1987 | J Urol1987，138，3： | 1 |
| 20 | The O'Conor technique: the gold standard for supratrigonal vesicova- ginal fistula repair显示文摘 | Nesrallah L J Srougi M Gittes R F | 1999 | J Urol1999，161，： | 1 |
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