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| 1 | Regulation of phenylpropanoid biosynthesis by MdMYB88 and MdMYB124 contributes to pathogen and drought resistance in apple显示文摘MdMYB88 and MdMYB124 have been demonstrated to be responsible for lignin accumulation in apple under drought stress.In this study,using a metabolomic approach,we identified differentially accumulated phenylpropanoid and flavonoid metabolites in MdMYB88/124 transgenic RNAi plants under control and long-term drought stress conditions in apple roots.We confirmed the regulation of phenylalanine by MdMYB88 and MdMYB124 via UPLC-MS in apple roots under both control and drought conditions.Using Electrophoretic Mobility Shift Assay(EMSA)and ChIPquantitative PCR(qPCR)analyses,we found that MdMYB88 positively regulates the MdCM2 gene,which is responsible for phenylalanine biosynthesis,through binding to its promoter region.Under long-term drought conditions,MdMYB88/124 RNAi plants consistently accumulated increased amounts of H2O2 and MDA,while MdMYB88 and MdMYB124 overexpression plants accumulated decreased amounts of H2O2 and MDA.We also examined the accumulation of metabolites in the phenylpropanoid biosynthesis pathway in the leaves of MdMYB88 and MdMYB124 transgenic apple plants after long-term drought stress.We found that metabolites responsible for plant defense,including phenylpropanoids and flavonoids,accumulated less in the RNAi plants but more in the overexpression plants under both control and drought conditions.We further demonstrated that MdMYB88/124 RNAi plants were more sensitive to Alternaria alternata f.sp.mali and Valsa mali,two pathogens that currently severely threaten apple production.In contrast,MdMYB88 and MdMYB124 overexpression plants were more tolerant to these pathogens.The cumulative results of this study provided evidence for secondary metabolite regulation by MdMYB88 and MdMYB124,further explained the molecular roles of MdMYB88 and MdMYB124 in drought resistance,and provided information concerning molecular aspects of their roles in disease resistance. | Dali Geng Xiaoxia Shen Yinpeng Xie Yusen Yang Ruiling Bian Yuqi Gao Pengmin Li Liying Sun Hao Feng Fengwang Ma Qingmei Guan | 2020 | Horticulture Research2020，7，1： | 5 |
| 2 | Online measurement of alkalinity in anaerobic co-digestion using linear regression method显示文摘Alkalinity is a reliable indicator of process stability in anaerobic digestion system.Total alkalinity(TA)and partial alkalinity(PA)are usually monitored offline as indicators for the status of anaerobic digestion process.In order to online monitor TA and PA,the linear regression method was used as estimator to predict alkalinity via software sensor method.Parameters,namely,pH,oxidation and reduction potential(ORP),and electrical conductivity(EC),were used as input variables.EC was the most significant parameter with TA and PA.Multiple linear regression(MLR)models and simple linear regression models with EC were constructed to predict TA and PA in anaerobic co-digestion system.On the basis of the evaluation of prediction accuracy,the applications of linear regression models were better for monitoring PA than TA.MLR models provided higher accuracy for alkalinity prediction than simple linear regression models.The two MLR models based on single-phase anaerobic digestion system were also feasible to predict TA in anaerobic co-digestion systems.However,the accuracy of these models should be improved by calibrating for broad applications of linear regression method in online alkalinity measurement. | Bai Xue Li Zifu Wang Xuemei He Xi Cheng Shikun Bai Xiaofeng Gao Ruiling | 2017 | International Journal of Agricultural and Biological Engineering2017，10，1： | 1 |
| 3 | Hexagonal superlattice state in dielectric barrier discharge显示文摘 | DONG Lifang GAO Ruiling HE Yafeng | 2006 | Phys Rev E2006，74，05： | 1 |
| 4 | Differential immunogenicity and protective efficacy of DNA vaccines expressing proteins of Mycobacterium tuberculosis in a mouse model 显示文摘 | Xionglin Fan Qian Gao Ruiling Fu | 2007 | Microbiological Research2007，4，： | 1 |
| 5 | Biochemical Methane Potentia Ⅰ of Blue - Green Algae in Biogas Fermentation Progress显示文摘 | XU Ruil GAO Tianrong | 2007 | Journal of Yunnan Normal University2007，27，5： | 1 |
| 6 | Differential immunogenicity and protective efficacy of DNA vaccines expressing proteins of Mycobacterium tuberculosis in a mouse model显示文摘 | Xionglin Fan Qian Gao Ruiling Fu | 2009 | Microbiol Res2009，164，： | 1 |
| 7 | Differential immunogenicity and protective efficacy of DNA vaccines expressing proteins of Mycobacterium tuberculosis in a mouse model显示文摘 | Xionglin Fan Qian Gao Ruiling Fu | 2007 | Microbiological Research2007，4，： | 1 |
| 8 | Research progress of siloxane removal from biogas显示文摘Siloxanes in biogas are detrimental to engine,turbine,fuel cell,etc.,thus it is necessary to remove siloxanes from biogas before biogas high-value utilization.At present,there are few domestic researches and related reports in view of siloxanes removal from biogas.This paper introduces the property of siloxanes as well as sampling and analysis method,and then presents the research progress of siloxanes removal from biogas.Three commercial technologies overseas are adsorption,absorption and cryogenic condensation.Among them,adsorption on activated carbon is the most widely used method.Other technologies,such as biological removal,catalytic processes,membranes,source controlling,etc.are under exploration and development.At last,this paper summarizes the advantages and disadvantages of siloxanes removal technologies as well as the applicability and analyzes the future research trend and emphasis.This paper could provide a reference in the field of biogas high-value utilization. | Gao Ruiling Cheng Shikun Li Zifu | 2017 | International Journal of Agricultural and Biological Engineering2017，10，1： | 0 |
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