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| 1 | Yeast knockout library allows for efficient testing of genomic mutations for cell-free protein synthesis显示文摘Cell-free protein synthesis(CFPS)systems from crude lysates have benefitted from modifications to their enzyme composition.For example,functionally deleting enzymes in the source strain that are deleterious to CFPS can improve protein synthesis yields.However,making such modifications can take substantial time.As a proof-of-concept to accelerate prototyping capabilities,we assessed the feasibility of using the yeast knockout collection to identify negative effectors in a Saccharomyces cerevisiae CFPS platform.We analyzed extracts made from six deletion strains that targeted the single deletion of potentially negative effectors(e.g.,nucleases).We found a statistically significant increase in luciferase yields upon loss of function of GCN3,PEP4,PPT1,NGL3,and XRN1 with a maximum increase of over 6-fold as compared to the wild type.Our work has implications for yeast CFPS and for rapidly prototyping strains to enable cell-free synthetic biology applications. | Jennifer A.Schoborg Lauren G.Clark Alaksh Choudhury C.Eric Hodgman Michael C.Jewett | 2016 | Synthetic and Systems Biotechnology2016，1，1： | 2 |
| 2 | 简并PCR扩增小瓜虫抑动抗原基因的ORF显示文摘抑动蛋白是小瓜虫(Ichthyophthiriusmultifiliis)体表纤毛的主要构成部分,也是宿主免疫系统发挥抗虫免疫时的主要识别抗原。根据已经报道的3种抑动蛋白N端及C端保守的多肽片断,设计了一对简并引物P6/P7,以小瓜虫分离株IchFJ9的基因组为模板,成功扩增了抑动蛋白编码区(ORF)的全长基因序列。扩增的iagFJ9基因全长1398bp,编码466个氨基酸,包含18个非标准密码子TAA(Glu,Q)推导的氨基酸序列包含6个以CPXGT为起始的串联重复单位,具有抑动蛋白的特征性结构。同源比较分析显示,扩增的iagFJ9基因与已知的小瓜虫抑动蛋白基因IAG52A基因具有88%同源率。证实了iagFJ9是一个新发现的抑动蛋白基因,用简并PCR技术去发现小瓜虫抑动蛋白基因家族新基因是可行的。 | 杨金先 陈强 刘晓东 林天龙 Theodore G.Clark | 2004 | 中国水产科学2004，11，2： | 2 |
| 3 | A worldwide phylogenetic classification of the Poaceae (Gramineae) Ⅲ: An update显示文摘We present an updated worldwide phylogenetic classification of Poaceae with 11783 species in 12 subfamilies,7 supertribes,54 tribes,5 super subtribes,109 subtribes,and 789 accepted genera.The subfamilies(in descending order based on the number of species)are Pooideae with 4126 species in 219 genera,15 tribes,and 34 subtribes;Panicoideae with 3325 species in 242 genera,14 tribes,and 24 subtribes;Bambusoideae with 1698 species in 136 genera,3 tribes,and 19 subtribes;Chloridoideae with 1603 species in 121 genera,5 tribes,and 30 subtribes;Aristidoideae with 367 species in three genera and one tribe;Danthonioideae with 292 species in 19 genera and 1 tribe;Micrairoideae with 192 species in nine genera and three tribes;Oryzoideae with 117 species in 19 genera,4 tribes,and 2 subtribes;Arundinoideae with 36 species in 14 genera and 3 tribes;Pharoideae with 12 species in three genera and one tribe;Puelioideae with 11 species in two genera and two tribes;and the Anomochlooideae with four species in two genera and two tribes.Two new tribes and 22 new or resurrected subtribes are recognized.Forty-five new(28)and resurrected(17)genera are accepted,and 24 previously accepted genera are placed in synonymy.We also provide an updated list of all accepted genera including common synonyms,genus authors,number of species in each accepted genus,and subfamily affiliation.We propose Locajonoa,a new name and rank with a new combination,L.coerulescens.The following seven new combinations are made in Lorenzochloa:L.bomanii,L.henrardiana,L.mucronata,L.obtusa,L.orurensis,L.rigidiseta,and L.venusta. | Robert J.Soreng Paul M.Peterson Fernando O.Zuloaga Konstantin Romaschenko Lynn G.Clark Jordan K.Teisher Lynn J.Gillespie Patricia Barberá Cassiano A.D.Welker Elizabeth A.Kellogg De-Zhu Li Gerrit Davidse | 2022 | Journal of Systematics and Evolution2022，60，3： | 1 |
| 4 | The I‐antigens of Ichthyophthirius multifiliis are GPI‐Anchored Proteins显示文摘 | THEODORE G.CLARK YANGAO JACEKGAERTIG XUTINGWANG GEORGINACHENG | 2005 | Journal of Eukaryotic Microbiology2005，，3： | 1 |
| 5 | Reconstruction of the anterior skull base with radial forearm free tissue transfer:case series and literature review显示文摘Aim:Reconstruction of the anterior skull base offers an especially complex challenge as the impermeable separation of the dural space and the upper aerodigestive tract must be maintained.We propose the use of the radial forearm free flaps(RFFF)as a superb method of re-establishing integrity in anterior skull base defects.Methods:Literature review and retrospective analysis of 4 single-institution cases of anterior skull base defects reconstructed with a RFFF.Data were collected on successful and unsuccessful defect repairs,complication rates,and length of hospitalization.Results:The indications for surgery were pneumocephalus,recurrent brain abscesses,recurrent frontal sinus mucoceles,and cerebrospinal fluid leak.Of the 4 cases,1 was complicated by a small dehiscence of the craniotomy site,1 developed infection,and 2 required further surgery.Conclusion:The use of RFFF is an excellent option for reconstruction of defects in the anterior skull base,especially those complicated by radiation,prior surgery,or infections.Patients with skull base defects are inherently at high risk for post-surgical complications.The RFFF transfers healthy,viable,well-vascularized tissue to prevent further infections and provides a reliable barrier between the dural and sinonasal spaces.This can reduce the need for repeat neurosurgical operations and hospitalizations. | Windy A.Olaya Lauren T.Daly Emily G.Clark Thomas Scholz Vincent Laurence Keyianoosh Z.Paydar Garrett A.Wirth Gregory R.D.Evans | 2016 | Plastic and Aesthetic Research2016，3，1： | 0 |
| 6 | 猪常见病毒性疾病显示文摘病毒可存在于机体组织的许多细胞里,影响猪只的不同系统:胃肠道系统、呼吸系统、繁殖系统、中枢神经系统和心脏,从而导致多种猪病。 | Sherrie G.Clark | 2011 | 今日养猪业2011，，1： | 0 |
| 7 | Grasses through space and time: An overview of the biogeographical and macroevolutionary history of Poaceae显示文摘Grasses are widespread on every continent and are found in all terrestrial biomes.The dominance and spread of grasses and grassland ecosystems have led to significant changes in Earth′s climate,geochemistry,and biodiversity.The abundance of DNA sequence data,particularly chloroplast sequences,and advances in placing grass fossils within the family allows for a reappraisal of the family′s origins,timing,and geographic spread and the factors that have promoted diversification.We reconstructed a time-calibrated grass phylogeny and inferred ancestral areas using chloroplast DNA sequences from nearly 90%of extant grass genera.With a few notable exceptions,the phylogeny is well resolved to the subtribal level.The family began to diversify in the Early–Late Cretaceous(crown age of 98.54 Ma)on West Gondwana before the complete split between Africa and South America.Vicariance from the splitting of Gondwana may be responsible for the initial divergence in the family.However,Africa clearly served as the center of origin for much of the early diversification of the family.With this phylogenetic,temporal,and spatial framework,we review the evolution and biogeography of the family with the aim to facilitate the testing of biogeographical hypotheses about its origins,evolutionary tempo,and diversification.The current classification of the family is discussed with an extensive review of the extant diversity and distribution of species,molecular and morphological evidence supporting the current classification scheme,and the evidence informing our understanding of the biogeographical history of the family. | Timothy J.Gallaher Paul M.Peterson Robert J.Soreng Fernando O.Zuloaga De-Zhu Li Lynn G.Clark Christopher D.Tyrrell Cassiano A.D.Welker Elizabeth A.Kellogg Jordan K.Teisher | 2022 | Journal of Systematics and Evolution2022，60，3： | 0 |
| 8 | 嗜热四膜虫表达载体的优化显示文摘近年来淡水纤毛虫嗜热四膜虫成为新的重组亚单位疫苗生产平台。为了进一步提高重组四膜虫的筛选效率,使其能更广泛地应用于重组亚单位疫苗制备,本研究利用基因工程手段,改造了原有的表达载体,把带有巴龙霉素抗性的neo基因换成嘌呤霉素抗性表达盒,从而提高重组四膜虫的筛选效率,达到优化表达载体的目的,同时将常用的氯化镉诱导的MTT1启动子替换成HSP70启动子,实现热诱导表达,为嗜热四膜虫表达平台在重组亚单位疫苗的进一步应用奠定基础。 | 刘晓东 陈强 朱春华 池洪树 龚晖 Theodore G.Clark | 2017 | 中国农学通报2017，33，32： | 0 |
| 9 | Integrating genomic and morphological data into bamboo taxonomicand evolutionary studies显示文摘The Bambusoideae (bamboos),with over 1700 described species,is the third largest subfamily of the Poaceae (grasses),native to all continents except Antarctica and Europe (Clark et al.,2015;Soreng et al.,2022).Within the Bambusoideae,three tribes representing the three major lineages are recognized.The Olyreae comprise the ca.130 species of herbaceous bamboos,which are smaller and much less lignified than their woody cousins. | Lynn G.Clark | 2023 | Plant Diversity2023，45，2： | 0 |
| 10 | Validation and Refinement of Two Interpretable Models for Coronavirus Disease 2019 Prognosis Prediction显示文摘Objective:To validate two proposed coronavirus disease 2019(COVID-19)prognosis models,analyze the characteristics of different models,consider the performance of models in predicting different outcomes,and provide new insights into the development and use of artificial intelligence(AI)predictive models in clinical decision-making for COVID-19 and other diseases.Materials and Methods:We compared two proposed prediction models for COVID-19 prognosis that use a decision tree and logistic regression modeling.We evaluated the effectiveness of different model-building strategies using laboratory tests and/or clinical record data,their sensitivity and robustness to the timings of records used and the presence of missing data,and their predictive performance and capabilities in single-site and multicenter settings.Results:The predictive accuracies of the two models after retraining were improved to 93.2% and 93.9%,compared with that of the models directly used,with accuracies of 84.3% and 87.9%,indicating that the prediction models could not be used directly and require retraining based on actual data.In addition,based on the prediction model,new features obtained by model comparison and literature evidence were transferred to integrate the new models with better performance.Conclusions:Comparing the characteristics and differences of datasets used in model training,effective model verification,and a fusion of models is necessary in improving the performance of AI models. | Kai Chang Ting Jia Ya-Na Zhou Zi-Xin Shu Ji-Fen Liu Jing Sun Qi-Guang Zheng Hao-Yu Tian Jia-Nan Xia Kuo Yang Ning Wang Hai-Long Sun Xin-Yan Wang Deng-Ying Yan Taane G.Clark Bao-Yan Liu Xiao-Dong Li Yong-Hong Peng Xue-Zhong Zhou | 2023 | World Journal of Traditional Chinese Medicine2023，9，2： | 0 |
| 11 | 西藏特有竹子Arundinaria macclureana的名实辨正(英文)显示文摘Arundinaria macclureana是在现代植物学中根据在西藏采集的标本描述的第一个竹子。其模式标本是1938年采集的,但只有花枝,没有地下茎、秆箨等重要特征。尽管如此,该种还成为了新属Borinda的模式种。在研究了其模式标本后,使本文第一作者联想起1991年在藏东南采集的西藏箭竹(Fargesia setosa)。西藏箭竹的模式标本采集于1977年,但只有营养体,没有花枝。在对比研究两个名称的模式后,确认二者实为同种。经与共同作者一起讨论,认为其正确学名为Fargesia macclureana,但该种所在的筱竹属群的关系还有待进一步的研究。 | 李德铢 Lynn G.CLARK Chris STAPLETON | 2006 | 云南植物研究2006，28，2： | 0 |
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