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| 1 | Transcription analysis of peloric mutants of Phalaenopsis orchids derived from tissue culture显示文摘Tissue culture has been widely used for mass propagation of Phalaenopsis. However, somaclonal variation occurred during micropropagation process posed a severe problem by affecting product quality. In this study, wild type and peloric flower buds of Phalaenopsis hybrids derived from flower stalk nodal culture were used for cDNA-RAPD and cDNA suppression subtractive hybridization analyses in order to study their genetic difference in terms of expressed sequence tags. A total of 209 ESTs from normal flower buds and 230 from mutants were sequenced. These ESTs sequences can be grouped into several functional categories involved in different cellular processes including metabolism, signal transduction, transcription, cell growth and division, protein synthesis, and protein localization, and into a subcat- egory of proteins with unknown function. Cymbidium mosaic virus transcript was surprisingly found expressed fre- quently in the peloric mutant of P. Little Mary. Real-time RT-PCR analysis on selected ESTs showed that in mutant flower buds, a bZIP transcription factor (TGA1a-like protein) was down-regulated, while up-regulated genes include auxin-regulated protein kinase, cyclophilin, and TCP-like genes. A retroelement clone was also preferentially expressed in the peloric mutant flowers. On the other hand, ESTs involved in DNA methylation, chromatin remodeling and post- transcriptional regulation, such as DNA methyltransferase, histone acetyltransferase, ERECTA, and DEAD/DEAH RNA helicase, were enriched in normal flower buds than the mutants. The enriched transcripts in the wild type indicate the down regulation of these transcripts in the mutants, and vice versa. The potential roles of the analyzed transcripts in the development of Phalaenopsis flowers are discussed. | Ya Huei CHEN Yi Jung TSAI Jian Zhi HUANG Fure Chyi CHEN | 2005 | Cell Research2005，15，8： | 21 |
| 2 | Evaluation of carisolv for the chemo-mechanical removal of primary root caries in vivo显示文摘 | Fure S Lingstrom P Birkhed D | 2002 | Caries Res2002，34，4： | 1 |
| 3 | Emotional symptoms in acute ischemie stroke显示文摘 | Fure B Wyller TB Engedal K | 2006 | Int J Geriatr Psychiatry2006，21，： | 1 |
| 4 | Fullerenes with metals inside显示文摘 | Chai Y Guo T Jin C Haufler RE Chibante LPF Fure J Wang L Alford J M Smalley R E | 1991 | J Phys Chem1991，95，： | 1 |
| 5 | Evaluation of Carisolv for the chemomechanical of primary root caries in vivo显示文摘 | Fure S Lingstrom P Birkhed D | 2000 | Caries Res2000，34，3： | 1 |
| 6 | Assessment of caries risk in elderly patients using the Cariogram model显示文摘 | Allan AY McNally ME Fure S | 2006 | J Can Dent Assoc2006，72，5： | 1 |
| 7 | Electrocardiographic and troponin T changes in acute ischaemic stroke 显示文摘 | Fure B Bruun TW Thommessen B | 2006 | Intern Med2006，259，6： | 1 |
| 8 | Electrocardiographic and tro poninT changes in acute ischaemic stroke显示文摘 | Fure B Bruun TW Thommessen B | 2006 | Intern Med2006，259，6： | 1 |
| 9 | Evaluation of Carisolv for the chemo-mechanical removal of primary root caries in vivo显示文摘 | Fure S Lingstrom P Birkhed D | 2000 | Caries Res2000，34，4： | 1 |
| 10 | Recentprogress on transcutaneous energy transfer for total artificial heart systems显示文摘 | FURES R VANDEVOORDE G | 2001 | Artificial Organs2001，25，5： | 1 |
| 11 | Reflectivity of laser-p roduced plasmas generated by a high intensity ultrashort pulse显示文摘 | Sauebrey R Fure J Bianc S P | 1994 | Phys Plasmas1994，1，5： | 1 |
| 12 | Electrocardiographic and troponin T changes in acute ischaemic stroke 显示文摘 | Fure B Bruun Wyller T Thommessen B | 2006 | J Intern Med2006，259，6： | 1 |
| 13 | Electrocardiographic and troponin T changes in acute ischemic stroke显示文摘 | Fure B BruunWyller TW Thommessen B | 2006 | J Intern Med2006，259，6： | 1 |
| 14 | Evaluation of carisiv for the chemomechanical removal of primary root caries in vivo 显示文摘 | Fure S Lingstrom P Bbiddaed D | 2000 | Caries Res2000，34，4： | 1 |
| 15 | Electrocardiographic and troponin T changes in acute ischaemic stroke 显示文摘 | Fure B Bruun Wyller T Thommessen B | 2006 | J Intern Med2006，259，6： | 1 |
| 16 | Electrocardiographic and troponin T changes in acute ischaemic stroke显示文摘 | Fure B Bruun TW Thommessen B | 2006 | Inter Med2006，259，6： | 1 |
| 17 | Depression, anxiety and other emotional symptoms after cerebral stroke显示文摘 | Fure B | 2007 | Tidsskr Nor Laegeforen2007，127，10： | 1 |
| 18 | DNAPL source depletion: linking architecture and flux response显示文摘 | Fure A D Jawitz J W Annable M D | 2006 | Journal of Contaminant Hydrology2006，85，： | 1 |
| 19 | Electrocardiographic and troponin T changes in acute ischemic stroke显示文摘 | Fure B Bruun Wyller T Thommessen B | | 0，，06： | 1 |
| 20 | Evaluation of carisolv for the chemo-mechanical removal of primary root caries in vivo显示文摘 | Fure S Lingstrom P Birkhed D | 2000 | Caries Res2000，34，： | 1 |
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