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| 1 | Studies on in vitro regeneration competence of pseudobulb cultures in Changnienia amoena Chien显示文摘An efficient procedure is outlined for rapid and mass propagation through in vitro culture of pseudobulbs collected in different seasons of an endangered orchid, Changnienia amoena Chien. Axillary buds formed on intact pseudobulbs (collected in April) after a 12-week incubation on half-strength Murashige and Skoog (1/2 MS) medium supplemented with 1 mg L-1 N6-benzyl- adenine (6-BA), 0.5 mg L-1 α-naphthaleneacetic acid (NAA), 100 ml L-1 coconut water and 0.8 g L-1 polyvinylpyrrolidone; no buds were observed on segmentalized pseudobulbs incubated on the same medium. The axillary buds obtained from pseudobulbs growing in the natural habitat in June were detached and incubated for 7 weeks on the same medium leading to 1.4 shoot buds per explant. With repeated subculturing of the shoots on 1/2 MS medium supplemented with 2 mg L-1 6-BA and 0.5 mg L-1 NAA, a mean of 3.3 shoot buds per explant were observed on successive shoot cultures. A mean of 4.5 roots per shoot were induced on the optimal root induction medium with 1/2 MS medium plus 1.0 mg L-1 NAA and 0.1 mg L-1 6-BA and the highest rooting per centage was 88.9%. Plantlets 4-5 cm in height were transplanted into pots containing a 1:1 humus and sand mixture and grown for 7 weeks in a greenhouse before being transferred to the field. The survival rate of these transplants was about 75% after two months of growth in the wild. | JIANG WeiMei ZHAO MingShui FU ChengXin | 2011 | Chinese Science Bulletin2011，56，24： | 8 |
| 2 | Growth, photosynthesis and podophyllotoxin accumulation of Dysosma versipellis in response to a light gradient and conservation implications显示文摘Dysosma versipellis (Hance) M. Cheng ex Ying (Berberidaceae) is a rare and vulnerable, perennial herb endemic to China with pharmaceutical significance. Increasing habitat loss and over-exploitation of the plant has severely affected the plant's in situ conservation, necessitating ex situ conservation and commercial cultivation. The light regime is a critical environmental factor contributing to successful ex situ conservation via efficient production of biomass and secondary metabolites. We investigated the responses of 2-year-old D. versipellis plants to a light gradient in terms of leaf morphology, growth, biomass production, photo- synthesis and podophyllotoxin (PTOX) accumulation. D. versipellis responded to the light gradient in terms of plant height, leaf size, leaf photosynthesis and PTOX accumulation. Plants demonstrated optimal vegetative growth, photosynthetic capability, and PTOX production, under a light intensity equal to 30% full sunlight. Half full sunlight led to the lowest production of PTOX despite having no obviously different effects on growth. Full sunlight was able to cause leaf death in mid-summer (July), although these plantlets did not significantly differ in growth or biomass production before July and new leaves emerged in August. D. versipellis is therefore proposed to be a shade-tolerant plant, well adapted to variations in irradiance. Irradiance of 10%-50%, particularly ca. 30% full sunlight is strongly recommended for ex situ conservation and commercial cultivation. | ZHAO YunPeng GONG HuaDong LU WeiQiang JIANG WeiMei ZHOU XiaoLong FU ChengXin | 2011 | Chinese Science Bulletin2011，56，24： | 6 |
| 3 | Control of postharvest Rhizopus rot of peach by microwavetreatment and yeast antagonist 显示文摘 | Hongyin Zhang Chengxin Fu Xiaodong Zheng | 2004 | Eur Food Res Technol2004，218，： | 1 |
| 4 | Postharvest biological control of gray mold rot of pear With Cryptococcus laurentii 显示文摘 | ZHANG Hongyin ZHENG Xiaodong FU Chengxin | 2005 | Postharvest Biology and Technology2005，35，： | 1 |
| 5 | Structural characterization of a 2-O-ace-tylglucomannan from Dendrobium officinalestem显示文摘 | Hua Yunfen Zhang Ming Fu Chengxin | 2004 | Carbohydrate Research2004，339，13： | 1 |
| 6 | Postharvest biological control of gray mold rot of pear with Cryptococcus laurentii 显示文摘 | Zhang Hongyin Zheng Xiaodong Fu Chengxin | 2005 | Postharvest Biology and Technology2005，35，1： | 1 |
| 7 | Genetic variation in the endangered and endemic species Changium smyrnioides 显示文摘 | Qiu Yingxiong Hong Deyuan Fu Chengxin | 2004 | Biochemical Systematics and Ecology2004，32，： | 1 |
| 8 | Control of postharvest Rhizopus rot of peach by microwave treatment and yeast antagonist显示文摘 | Chengxin Fu Xiaodong Zheng | 2004 | Eur Food Res Technol2004，218，： | 1 |
| 9 | Plant and fungal species interactions differ between aboveground and belowground habitats in mountain forests of eastern China显示文摘Plant and fungal species interactions drive many essential ecosystem properties and processes;however,how these interactions differ between aboveground and belowground habitats remains unclear at large spatial scales.Here,we surveyed 494 pairwise fungal communities in leaves and soils by Illumina sequencing,which were associated with 55 woody plant species across more than 2,000-km span of mountain forests in eastern China.The relative contributions of plant,climate,soil and space to the variation of fungal communities were assessed,and the plant-fungus network topologies were inferred.Plant phylogeny was the strongest predictor for fungal community composition in leaves,accounting for 19.1%of the variation.In soils,plant phylogeny,climatic factors and soil properties explained 9.2%,9.0%and 8.7%of the variation in soil fungal community,respectively.The plant-fungus networks in leaves exhibited significantly higher specialization,modularity and robustness(resistance to node loss),but less complicated topology(e.g.,significantly lower linkage density and mean number of links)than those in soils.In addition,host/fungus preference combinations and key species,such as hubs and connectors,in bipartite networks differed strikingly between aboveground and belowground samples.The findings provide novel insights into cross-kingdom(plant-fungus)species co-occurrence at large spatial scales.The data further suggest that community shifts of trees due to climate change or human activities will impair aboveground and belowground forest fungal diversity in different ways. | Teng Yang Leho Tedersoo Pamela S.Soltis Douglas E.Soltis Miao Sun Yuying Ma Yingying Ni Xu Liu Xiao Fu Yu Shi Han-Yang Lin Yun-Peng Zhao Chengxin Fu Chuan-Chao Dai Jack A.Gilbert Haiyan Chu | 2023 | Science China(Life Sciences)2023，66，5： | 0 |
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