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| 1 | Isolation and characterisation of human gingival margin-derived STRO-1/MACS^+ and MACS^-cell populations显示文摘Recently,gingival margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting(MACS) showed remarkable periodontal regenerative potential in vivo.As a second-stage investigation,the present study's aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive(MACS^+) and STRO-1-negative(MACS^-) cell populations from the human free gingival margin.Cells were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 antibodies.Subsequently,the MACS^+ and MACS^- cell fractions were characterized by flow cytometry for expression of CD14,CD34,CD45,CD73,CD90,CD105,CD146/MUC18 and STRO-1.Colony-forming unit(CFU) and multilineage differentiation potential were assayed for both cell fractions.Mineralisation marker expression was examined using real-time polymerase chain reaction(PCR).MACS^+ and MACS- cell fractions showed plastic adherence.MACS^+ cells,in contrast to MACS- cells,showed all of the predefined mesenchymal stem/progenitor cell characteristics and a significantly higher number of CFUs(P<0.01).More than 95%of MACS^+ cells expressed CD105,CD90 and CD73;lacked the haematopoietic markers CD45,CD34 and CD14,and expressed STRO-1 and CD146/MUC18.MACS- cells showed a different surface marker expression profile,with almost no expression of CD14 or STRO-1,and more than 95%of these cells expressed CD73,CD90 and CD146/MUC18,as well as the haematopoietic markers CD34 and CD45 and CD105.MACS^+ cells could be differentiated along osteoblastic,adipocytic and chondroblastic lineages.In contrast,MACS- cells demonstrated slight osteogenic potential.Unstimulated MACS^+ cells showed significantly higher expression of collagen I(P<0.05) and collagen III(P<0.01),whereas MACS^- cells demonstrated higher expression of osteonectin(P<0.05;MannWhitney).The present study is the first to compare gingival MACS^+ and MACS- cell populations demonstrating that MACS^+ cells,in contrast to MACS- cells,harbour stem/progenitor cell characteristics.This study also validates the effectiveness of the STRO-l/MACS^+technique for the isolation of gingival stem/progenitor cells.Human free gingival margin-derived STRO-1/MACS^+ cells are a unique renewable source of multipotent stem/progenitor cells. | Karim M Fawzy El-Sayed Sebastian Paris Christian Graetz Neemat Kassem Mohamed Mekhemar Hendrick Ungefroren Fred Fndrich Christof Drfer | 2015 | International Journal of Oral Science2015，7，2： | 6 |
| 2 | Comparative analysis of Solanum stoloniferum responses to probing by the green peach aphid Myzus persicae and the potato aphid Macrosiphum euphorbiae显示文摘抽象植物由种类特定的防卫机制保护自己免于蚜虫攻击。以前,我们证明了茄属 stoloniferum Schlechtd 有抵抗因素到 Myzus persicae Sulzer (Homoptera:Aphididae ) 在 epidermal/mesophyll 水平,那不对 Macrosiphum euphorbiae 托马斯是有效的(Homoptera:Aphididae ) 。这里,我们比较 nymph 的死亡, pre 繁殖的发展时间,和 M 的探查的行为。persicae 和 M。S 上的 euphorbiae。stoloniferum 和茄属 tuberosum L。而且,我们在 S 在基因表示分析变化。stoloniferum 96 个小时由也张贴群袭蚜虫种类。尽管 M。探查蚜虫遇到更多的探查的行为表演的 euphorbiae 在韧皮部 activitieslonger 在 S 上探查和分泌唾液时间上抑制。stoloniferum 比在 S 上。tuberosum,蚜虫成功了在两植物上到达韧皮部树液的持续摄取。由 M 探查。S 上的 persicae。stoloniferum 植物导致了仅仅的有限的喂。M 的幸存。euphorbiae 和 M。persicae 在年轻叶子,然而并非在 S 的衰老的叶子被影响。stoloniferum。由 M 的群袭。euphorbiae 比 M 改变了更多的基因的表示。persicae 做了。在全身的水平,两个蚜虫得到了弱回答。S 的群袭。有很多 M 的 stoloniferum 植物。persicae 在叶子导致了词法变化,导致被破坏脉管的实质引起并且包围织物的色点的发展。相反,由 M 的群袭。euphorbiae 没有词法效果。两个都,植物种类能为 M 被认为是好主人。euphorbiae,而仅仅 S。tuberosum 是为 M 的一位好主人。persicae 和 S。stoloniferum 不是。S 的群袭。由 M 的 stoloniferum。persicae 或 M。euphorbiae 象一套普通基因一样改变了为每蚜虫特定的一套植物基因的表示。 | Adriana E. Alvarez Viviana G. Broglia Anahi M. Alberti D'Amato Doret Wouters Edwin van der Vossen Elisa Garzo W. Fred Tjallingii Marcel Dicke Ben Vosman | 2013 | Insect Science2013，20，2： | 5 |
| 3 | Genome resequencing and transcriptome profiling reveal structural diversity and expression patterns of constitutive disease resistance genes in Huanglongbing-tolerant Poncirus trifoliata and its hybrids显示文摘Huanglongbing(HLB)is the most destructive bacterial disease of citrus worldwide.While most citrus varieties are susceptible to HLB,Poncirus trifoliata,a close relative of Citrus,and some of its hybrids with Citrus are tolerant to HLB.No specific HLB tolerance genes have been identified in P.trifoliata but recent studies have shown that constitutive disease resistance(CDR)genes were expressed at much higher levels in HLB-tolerant Poncirus hybrids and the expression of CDR genes was modulated by Candidatus Liberibacter asiaticus(CLas),the pathogen of HLB.The current study was undertaken to mine and characterize the CDR gene family in Citrus and Poncirus and to understand its association with HLB tolerance in Poncirus.We identified 17 CDR genes in two citrus genomes,deduced their structures,and investigated their phylogenetic relationships.We revealed that the expansion of the CDR family in Citrus seems to be due to segmental and tandem duplication events.Through genome resequencing and transcriptome sequencing,we identified eight CDR genes in the Poncirus genome(PtCDR1-PtCDR8).The number of SNPs was the highest in PtCDR2 and the lowest in PtCDR7.Most of the deletion and insertion events were observed in the UTR regions of Citrus and Poncirus CDR genes.PtCDR2 and PtCDR8 were in abundance in the leaf transcriptomes of two HLB-tolerant Poncirus genotypes and were also upregulated in HLB-tolerant,Poncirus hybrids as revealed by real-time PCR analysis.These two CDR genes seem to be good candidate genes for future studies of their role in citrus-CLas interactions. | Nidhi Rawat Brajendra Kumar Ute Albrecht Dongliang Du Ming Huang Qibin Yu Yi Zhang Yong-Ping Duan Kim D Bowman Fred G Gmitter Jr Zhanao Deng | 2017 | Horticulture Research2017，4，1： | 5 |
| 4 | 用功率流方法研究压电陶瓷变压器的电气特性显示文摘基于功率流方法 ,本文提出了一种分析压电陶瓷变压器电气特性的方法 .其主要贡献是 :(1)给出了计算最佳负载、电压增益、效率和输出功率等电气特性的公式 ;(2 )利用这些公式 ,给出了分析压电陶瓷变压器电气特性的计算程序 ,并提出了最佳电阻和最佳匹配网络的概念 ;(3)为了证明其理论结果 ,文中给出了仿真结果 .本文所得到的结果为分析压电陶瓷变压器电气特性提供了一个有用的工具 . | 张东彦 张卫平 D Y Chen Fred C Lee | 2003 | 电子学报2003，31，11： | 2 |
| 5 | Customer portfolio management: Toward a dynamic theory of exchange relationships显示文摘 | Johnson Michael D and Selnes Fred | 2004 | Journal of Marketing2004，68，2： | 1 |
| 6 | A Theoretical Extension of the Technology Acceptance Model: Four Longitudinal Field Studies 显示文摘 | Viswanath V Fred D D | 2000 | Management Science2000，46，2： | 1 |
| 7 | A wave generation toolbox for the open-source CFD library: OpenFOAM? 显示文摘 | JACOBSEN N G FUHRMAN D R FREDS E J | 2012 | International Journal for Numerical Methods in Fluids2012，70，9： | 1 |
| 8 | Epoxy silanes in reactive polymer emulsions 显示文摘 | Ming J C Fred D O Antonio C | 1997 | Journal of Coatings Technology1997，69，： | 1 |
| 9 | Gelation of casein-whey protein mixtures显示文摘 | VASBINDER A J FRED V D V | 2004 | J Dairy Sci2004，87，： | 1 |
| 10 | Mycorrhizal fungi enhance accumulation and tolerance of chromium in sunflower (Hellanthus annuus ) 显示文摘 | Fred T D Jeffrey D P Ronald J N | 2001 | Journal of Plant Physiology2001，158，6： | 1 |
| 11 | Decontamination of water by excimer UV radiation显示文摘 | Fred C D | 2002 | IEEE Transactions on Plasma Science2002，30，4： | 1 |
| 12 | The probabilistic data association fil- ter显示文摘 | Yaakov B S Fred D Jim H | 2009 | IEEE Control Systems Magazine2009，29，6： | 1 |
| 13 | Spinning disk interferometry the biocd (1) 显示文摘 | David D Nolte Fred E Regnier | 2004 | Optics & Photonics News2004，，10： | 1 |
| 14 | Peanut allergy and anapbylaxis显示文摘 | Fred D Finkelman | 2010 | Current Opinion in Immunology2010，22，： | 1 |
| 15 | Integrated approach to teaching and testing in histology with real and virtual im- aging显示文摘 | Heidger PM Fred D Daniel C | 2002 | Anat Rec2002，269，2： | 1 |
| 16 | Nonlinear filters: Beyond Kalman filter显示文摘 | Fred D | 2005 | IEEE Aero Elec Systems Magzine2005，43，8： | 1 |
| 17 | User acceptance of information technology: systemcharacteristics, user perceptions, and behavioral impacts 显示文摘 | Davis Fred D | 1993 | International Journal of Man-Machine Studies1993，38，3： | 1 |
| 18 | High speed label-free detection by spinning disk micro-interferometry 显示文摘 | Manoj M Varma Halina D Inerowicz Fred E Regnier | 2004 | Biosensors and Bioelectronics2004，19，11： | 1 |
| 19 | SCTP What,Why and How显示文摘 | Preethi N Fred B Paul D A | 2009 | IEEE IntemetComputing2009，13，5： | 1 |
| 20 | Complication of therapeutic laparoscopy显示文摘 | Fred B Sandor D Stanley K | 1994 | Curr Probl Surg1994，31，： | 1 |
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