维普期刊资源整合服务平台（V6.5）

98篇 您的检索式：作者名="Fish W"

选中清除导出查看参考文献查看引证文献引用追踪共5页首页上一页第1页下一页末页 /5 跳转

	题名	作者	年代	出处	被引量
1	Development of a laser capture microscope-based single-cell-type proteomics tool for studying proteomes of individual cell layers of plant roots显示文摘Single-cell-type proteomics provides the capability to revealing the genomic and proteomics information at cell-level resolution.However,the methodology for this type of research has not been well-developed.This paper reports developing a workflow of laser capture microdissection(LCM)followed by gel-liquid chromatography-tandem mass spectrometry(GeLC-MS/MS)-based proteomics analysis for the identification of proteomes contained in individual cell layers of tomato roots.Thin-sections(~10-μm thick,10 sections per root tip)were prepared for root tips of tomato germinating seedlings.Epidermal and cortical cells(5000–7000 cells per tissue type)were isolated under a LCM microscope.Proteins were isolated and then separated by SDS–polyacrylamide gel electrophoresis followed by in-gel-tryptic digestion.The MS and MS/MS spectra generated using nanoLC-MS/MS analysis of the tryptic peptides were searched against ITAG2.4 tomato protein database to identify proteins contained in each single-cell-type sample.Based on the biological functions,proteins with proven functions in root hair development were identified in epidermal cells but not in the cortical cells.Several of these proteins were found in Al-treated roots only.The results demonstrated that the cell-type-specific proteome is relevant for tissue-specific functions in tomato roots.Increasing the coverage of proteomes and reducing the inevitable cross-contamination from adjacent cell layers,in both vertical and cross directions when cells are isolated from slides prepared using intact root tips,are the major challenges using the technology in proteomics analysis of plant roots.	Yingde Zhu Hui Li Sarabjit Bhatti Suping Zhou Yong Yang Tara Fish Theodore W Thannhauser	2016	Horticulture Research2016，3，1：	2
2	Mapping frost resistance loci in wheat and comparative mapping with other cereals显示文摘	Snape J W Semikhodskii A FISH L	1997	Acta Agronomic Hungary1997，45，：	1
3	A quantitative assay for lycopene that utilizes reduced volumes of organic solvents显示文摘	FISH W W PERKINS-VEAZIE P COLLINS J K	2002	Journal of Food Composition and Analysis2002，15，：	1
4	Aspm specifically maintains symmetric proliferative divisions of neuroepithelial cells 显示文摘	Fish JL Kosodo Y Enard W	2006	Proc Natl Aead Sci USA2006，103，10：	1
5	Role of Brownian motion hydrodynamics on nanofluid thermal conductivity 显示文摘	EVANS W FISH J KEBLINSKI P	2006	Appl Phy Lett2006，88，：	1
6	Rapid hexane free method for analyzing lycopene content in watermelon显示文摘	DAVIS A R FISH W W PERKINS-VEAZIE P	2003	Journal of Food Science2003，68，：	1
7	Redox interactions of Cr ( VI ) and substituted phenols:Products and mechanism 显示文摘	Elovitz M S Fish W	1995	Environmental Science & Technology1995，29，8：	1
8	CP117 and Shear Connectors in Steel-Concrete Composite Beams Made with Normal-density or Light-Wight Concrete显示文摘	John W Fish	1971	The Structure Eng1971，49，3：	1
9	Basic local alignment search tool 显示文摘	Fish W Miller EW	1990	J Mol Biol1990，215，：	1
10	A quantitative assay for lyeopene that utilizes reduced volumes of organic solvents显示文摘	Fish W W Perkins - Veazie P Collins J K	2002	Jour- nal of Food Composition and Analysis2002，15，：	1
11	Redox interaction of Cr( Ⅵ ) and substituted phenols:Kinetic investigation 显示文摘	Elovitz M S Fish W	1994	Environ Sci Techno11994，28，12：	1
12	Cooperating Security Managers:A Peerbased Intrusion Detection System显示文摘	White G B Fish E A Pooch U W	1996	IEEEE Network1996，10，1：	1
13	Perceptions of parents of students with autism towards the IEP meeting: A case study of one family support group chap- ter显示文摘	Fish W W	2006	Education2006，27，1：	1
14	Binary codes from the line graph of the n-cube显示文摘	Fish W Key J D Mwambene E	2010	Journal of Symbolic Computation2010，45，：	1
15	The infuence of rootstock selection on fruit quality attributes of watermelon 显示文摘	Bruton B D Fish W W Roberts W	2009	Open Food Science Journal2009，3，：	1
16	Degradation of carbon tetrachloride by iron metal: Complexation effects on the oxide surface显示文摘	Johnson T L Fish W Gorby Y A	1998	Journal of Contaminant Hydrology1998，29，：	1
17	Issues in searching moleeular sequence databases显示文摘	ALTSCHUL S F BOGUSKI M S FISH W		0，，06：	1
18	Knock:Flame Acceleration or Spontaneous Ignition显示文摘	Affleck W S Fish A	1968	Combustion and Flame1968，12，3：	1
19	Landscape characterzation of peridomestic risk for Lyme disease using satellite magery显示文摘	Dister S W Fish D Bros S	1997	Am J Trop Med Hyg1997，57，：	1
20	A rapid spectrophotometric method for analyzing lycopene content in tomato and tomato products显示文摘	Davis R Fish W Perkins Veazie P	2003	Postharvest Biology and Technology2003，28，3：	1

返回顶部每页显示：

共5页首页上一页第1页下一页末页 /5 跳转

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

用户提示