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| 1 | Intergrin gene expression profiles of human hepatocellular carcinoma显示文摘AIM: To investigate gene expression profiles of intergringenes in hepatocellular carcinoma (HCC) through theusage of Atlas Human Cancer Array membranes, semi-quantitative reverse transcription polymerase chainreaction (RT-PCR) and Northern blot.METHODS: Hybridization of cDNA array membrane wasperformed with α 32P-labeled cDNA probes synthesizedfrom RNA isolated from hepatocellular carcinoma andadjacent non-cirrhotic liver. AtlasImage, which is asoftware specific to array, was used to analyze theresult. RT-PCR of 24 pairs specimen and Northern blotof 4 pairs specimen were used to confirm the expressionpattern of some intergrin genes identified by Atlasarrays hybridization.RESULTS: Among 588 genes spotted in membrane, 17genes were related to intergrin. Four genes were up-regulated, such as intergrin alpha8, beta1, beta7 andbeta8 in HCC. Whereas there were no genes down-regulated in HCC. RT-PCR and Northern blot analysisof intergrin beta1 gene gave results consistent withcDNA array findings.CONCLUSION: Investigation of these intergrin genesshould help to disclose the molecular mechanism of thecell adhesion, invasive and metastasis of HCC. A fewgenes are reported to have changed in HCC for the firsttime. The quick and high-throughout method of profilinggene expression by cDNA array provides us overviewof key factors that may involved in HCC, and may findthe clue of the study of HCC metastasis and moleculartargets of anti-metastasis therapy. The preciserelationship between the altered genes and HCC is amatter of further investigation. | Lian-Xin Liu Hong-Chi Jiang Wei-Hui Zhang An-Long Zhu Department of Surgery,the First Clinical College,Harbin Medical University,Harbin 150001,Heilongjiang Province,China Lian-Xin Liu Zhi-Hua Liu Jing Zhou Xiu-Qin Wang Min Wu National Laboratory of Molecular Oncology,Department of Cell Biology,Cancer Institute,Chinese Academy of Medical Science & Peking Union Medical College,Beijing 100021,China | 2002 | World Journal of Gastroenterology2002，8，4： | 29 |
| 2 | Deficit of mitochondria-derived ATP during oxidative stress impairs mouse MII oocyte spindles显示文摘尽管在母亲的老化和不孕的氧化应力的角色被建议了,内在的机制充分没被理解。现在的学习被设计在氧化应力下面在中期 II (MII ) 卵母细胞决定在 mitochondrial 功能和锭子稳定性之间的关系。MII 老鼠卵母细胞当面与 H2O2 被对待或渗透转变毛孔(PTP ) 的缺席块 ers cyclosporin A (CsA ) 。另外,抗氧化剂 N-acetylcysteine (NAC ) , F0/F1 synthase 禁止者 oligomycin A,线粒体 uncoupler 羰基氰化物 4-trifluoro-methoxyphenylhydrazone (FCCP ) 或加 2.5 公里 Ca2+(Th+2.5 公里 Ca2+) 的 thapsigargin 在机械学的研究被使用。卵母细胞锭子和染色体的词法分析被执行并且 mitochondrial 膜潜力(DeltaPsim ) ,细胞质的免费的钙集中([Ca2+] c ) 并且在卵母细胞以内的细胞质的 ATP 内容也是 assayed。在一时间 -- 并且 H2O2 剂量依赖者举止,锭子在卵母细胞以后被发现的 meiotic 的混乱与 H2O2 被对待,它被预告的处理与 NAC 阻止。H2O2 的管理导致了 DeltaPsim 的驱散,的增加[Ca2+] 在细胞质的 ATP 的 c 和减少铺平。到 H2O2 处理的卵母细胞的这些有害回答能被孵化前与 CsA 堵住。类似于驱散的 H2O2,两 oligomycin A 和 FCCP DeltaPsim,减少的细胞质的 ATP 内容和拆卸的 MII 卵母细胞锭子,当时高[Ca2+] c 独自没在锭子形态学上有效果。在结论,在导出线粒体的 ATP 的减少可以在氧化应力期间引起老鼠 MII 卵母细胞锭子的拆卸,大概由于 mitochondrial PTP 的开始。 | Xiao Zhang~(1,2,*) Xue Qing Wu~(1,3,*) Shuang Lu~(4,5,6) Ying Lu Guo~1 Xu Ma~(4,5,6) ~1Peking University First Hospital,Institute of Urology of Peking University,Beijing 100034,China ~2Reproductive and Genetic Center of Peking University First Hospital,Beijing 100034,China ~3Department of Obstetrics and Gynecology,Shanxi Medical University Second Hospital,Taiyuan 030000,China ~4Reproductive and Genetic Center of National Research Institute for Family Planning,Beijing 100038,China ~5Department of Genetics, Peking Union Medical College, Beijing 100038. China ~6 WHO Collaborative Center for Research in Human Reproduction,Beijing 100038,China | 2006 | Cell Research2006，16，10： | 24 |
| 3 | Relationship between petroleum hydrocarbon and plankton in a mesocosm experiment显示文摘A mesocosm experiment was carried out from May 26 to June 1, 1998 at the location of 30’52’N, 122’36’E, the Changjiang Estuary, in order to measure the influence of petroleum hydrocar- bon to the ecosystem, furthermore, to estimate the environmental capacity. In the experiment, it is found that the petroleum hydrocarbon can, in some degree, enhance the growth of diatom, but inhibit the growth of dinoflagellate. In general, the petroleum hydrocarbon can inhibit the growth of both Phytoplankton and zooplankton, and can the inhibit the growth of total plankton (including phytoplankton and zooplankton) as well. A kinetic model was presented to estimate the uptake/release rate constants of petroleum hydrocarbon by plankton, and thereafter, the uptake and release rate constants (k1, k2), bioconcentration factor (BCF) as well as the petroleum bydrocarbon influenced influenced uptake and release rate constants of nutrients by phytoplankton (kup, krel) were obtained. The results imply that the bioconcentration of petroleum hydrocarbon by plankton is fairly large and petroleum hydrocarbon caused no significant influence on the uptake of N- NO3, but significant influence on that of P -PO4. In addition, the application of kinetic model for the bioconcentration of volatile organic toxic compound by organism suggests that the uptake of petroleum hydrocarbon by plankton was an important process for the environmental capacity of petroleum hydrocarbon. | Shi Xiaoyong, Wang Xiulin, Han Xiurong, Jiang Yu Zhu Mingyuan, Chen Shang, Koshikawa Hiroshi (1. Ocean University of Qingdao, Qingdao 266003, China 2. First Institute of Oceanology, Qingdao 266061, China 3. National Institute for Environmental Studies, Ja | 2001 | Acta Oceanologica Sinica2001，20，2： | 12 |
| 4 | Expression of p53, p16 and COX-2 in pancreatic cancer with tissue microarray显示文摘BACKGROUND: Pancreatic cancer development and progression is driven by the accumulation of genetic changes. In this study we constructed tissue microarray containing specimens from pancreatic cancer, adjacent non-cancer tissue and normal tissue to survey the expression of p53, p16 and cyclooxyganase-2 (COX-2). METHODS: Tissue microarray containing 337 specimens from different stages of pancreatic cancer, adjacent noncancer tissue and normal tissues was constructed, and the expression of p53, p16 and COX-2 was assayed by immunohistochemistry to consecutive formalin-fixed tissue microarray sections. RESULTS: The expression of p53, p16 and COX-2 was significantly higher in tumorous tissues than in non-tumorons ones. A significant relationship was observed between p53 and COX-2, or p16 and COX-2. But no obvious correlation was seen between p53 and p16 expressions. Logistic regression analysis showed p53 and COX-2 as dependent predictors in pancreatic carcinogenesis, and a reciprocal relationship to neoplastic progression between p53 and COX-2. CONCLUSION: Combination analysis of p53 and COX-2 may be useful in predicting pancreatic carcinogenesis. | Lei Xu, You-Ming Li, Chao-Hui Yu, Lan Li, You-Shi Liu, Bao-Feng Zhang, Jing Fang, Qiong Zhou, Ying Hu and Hen-Jun Gao Department of Gastroenterology, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China and National Engineering Center for Biochip, Shanghai 200000, China | 2006 | Hepatobiliary & Pancreatic Diseases International2006，5，1： | 7 |
| 5 | Semi-analytical analysis of the response of the air temperature over the land surface to the global vegetation distribution显示文摘Response of the air temperature over the land surface to the global vegetation distribution is investigated, using a three-dimensional governing equation to simulate the steady, large-scale, limited amplitude perturbation of the free, inviscid and adiabatic atmosphere. The adoption of the static equation leads to a temperature governing equation in the terrain following coordinate. With the prescribed temperature as the upper boundary condition and the radiation balance as the lower boundary condition, the semi-analytical solution of the global circulation temperature can be calculated. In this article, only the air temperature (at 2 m height) over the land surface is analyzed, and the result suggests that this model can simulate the air temperature pattern over the land surface reasonably. A better simulation occurs when a simple feedback of the albedo on the temperature is included. Two sensitivity experiments are analyzed through this model. One suggests that the air temperature over the land surface descends obviously when the land surface is covered with ice all over, while another suggests that the air temperature rises a little when the land surface is covered with forest except the ice-covered area. This model appears to be a good tool to study the response of the air temperature to the vegetation distribution. Limitations of the model are also discussed. | LIU Fei1,2 & CHAO JiPing3,4 1 Institute of Atmospheric Physics, Chinese Academy of Sciences, Beijing 100029, China 2 Graduate University of Chinese Academy of Sciences, Beijing 100049, China 3 National Marine Environmental Forecasting Center, Beijing 100081, China 4 First Institute of Oceanography, State Oceanic Administration of China, Qingdao 266061, China | 2009 | Chinese Science Bulletin2009，54，14： | 2 |
| 6 | 2004年第一次全国经济普查显示文摘普查是国家为了取得某项重大国情国力资料,而专门组织的一次性大规模全面调查。新中国成立以来,我国已经先后进行了12次全国性普查。1994年,我国开始实行周期性的普查制度。国家实行周期性的普查制度,定期取得全国人口、农业、工业、第三产业和基本单位等方面的基础数据,客观全面地反映重大国情国力的发展变化情况,为研究制定国民经济和社会发展规划及相关政策提供基础性资料,保障党中央和国务院各项决策的正确性和有效性是十分必要的,同时也为国民经济核算和统计调查体系改革奠定了基础。但是同时也暴露出一些问题,如普查项目设置过多,普查的时间比较分散和普查涵盖面不全等。因此,国家统计局、国家发展改革委员会、财政部共同研究,建议对国家普查项目的设置和周期安排作适当调整。2003年12月2日,国务院向各省、自治区、直辖市人民政府和国务院各部委。 | the Office of the First National Economic Census,State Council Wu Hui | 2004 | 中国经济景气月报2004，0，1： | 1 |
| 7 | Preventing unintentional injuries in Indigenous children and youth in Canada显示文摘 | Banerji A Canadian Paediatric Society First Nations Inuit and Metis Health Committee | 2012 | Peadiatr Child Health2012，17，7： | 1 |
| 8 | Multiple equilibria of cross-equatorial Inertial jets显示文摘Based on the developed Anderson and Moore's theory about cross-equatorial inertial jets and a nonlinear equivalence shallow water model, new universal functions are determined by the characters of the vortical large-scale air flow (atmosphere) or ocean current (ocean) related to the jet, then the potential vorticity and energy conservation equations along the streamline in the cross-equatorial in-ertial jets can be obtained. Because the governing equations are nonlinear, some limited multiple equi-libria of cross-equatorial inertial jets may exist. According to the character of large-scale air flow or ocean current outside the jets, the existent criterion for multiple eqnilibria in cross-equatorial inertial jets is discussed, and two examples for multiple equilibia of nonlinear governing equations are given. | CHAO JiPing1,2 & LIU Fei3,4 1 National Marine Environmental Forecasting Center, Beijing 100081, China 2 The First Institute of Oceanography ,SOA, Qingdao 266060, China 3 Key Laboratory Regional Climate-Environment for Temperate East Asia, Institute of Atmospheric Physics, Chinese Academy of Sciences, Beijing 100029, China 4 Graduate University of Chinese Academy of Sciences, Beijing 100039, China | 2007 | Science China Earth Sciences2007，50，1： | 1 |
| 9 | Safe and Objective Assay of Enterovirus 71 Neutralizing Antibodies via Pseudovirus显示文摘Current serum neutralization assays based on the inhibition of the cytopathic effect(Nt-CPE) need to ma nipulate live viruses, which are time-consuming, labor-intensive, and have the potential exposure to infectious agents, so a safe and objective assay via pseudovirus for the fast and efficient detection of enterovirus 71(EV71) neutralizing antibodies was developed. First, we generated EV71 pseudovirus containing firefly luciferase gene in place of the capsid gene P1 in EV71 genome. Vero cells infected with 200 CCID50(50% cell culture infective dose) of EV71 pseudovirus for 24 h were found to have the best performance. Seval sera were measured by EV71 pseudoparticle neutralization assay(Nt-PPN) and the conventional serological method Nt-CPE. Neutralizing antibody titers measured by Nt-PPN and those obtained by Nt-CPE demonstrate a high correlation between the two methods. Overall, the PPN assay represents a valid alternative to conventional serological methods for the evaluation of EV71 neutralizing anti bodies. This method can be used for detecting neutralizing antibodies of other picornaviruses, such as hepatitis A vi rus(HAV) and coxsackievirus 16(CVA16), and make it possible to determine whether there is cross-reactivity be tween EV71 and CVA16. | JIN Jun1, XU Lin1, GUO Shi-jie2, SUN Shi-yang1, ZHANG Shu1, ZHU Chang-lin3, KONG Wei1 and JIANG Chun-lai1 1. National Engineering Laboratory for AIDS Vaccine, College of Life Science, Jilin University, Changchun 130012, P. R. China 2. Department of Pediatrics, the First Hospital of Jilin University, Changchun 130021, P. R. China 3. Changchun Baike Biotechnology Co., Changchun 130012, P. R. China | 2012 | Chemical Research in Chinese Universities2012，28，1： | 1 |
| 10 | Vitamin D supplementation:recommendations for Canadian mothers and infants显示文摘 | FIRST NATIONS I COMMITTEE M H | 2007 | Paediatr Child Health2007，12，7： | 1 |
| 11 | Genre Analysis on the Abstracts of Graduation Papers Written by Pre-service Teachers of English显示文摘As a particular genre,Abstract is a growing field of study in linguistics. Move analysis is one aspect that has conducted to describe RA abstract. This article analyzed the particular genre with the purpose of helping them work out good abstracts for academic writing to improve their ability of doing research. One hundred abstracts were selected randomly from all the 450 graduation papers written by Eng lish-major pre-service teachers in 2010 in Hunan First Normal University in China. These informative abstracts were analyzed with the framework of Hyland's 2000 Model. The results revealed that these English-major pre-service teachers haven't done well in the abstract writing for their graduation paper, most of whom have no good understanding about the move model and move sequence, which impli cates that explicit introduction of terms of genre, move and move sequence etc., and training on how to write good abstracts in future study are really needed. | ZHOU Chao-ying 1 , CHEN Jun 2 (1. Foreign Language Department, Hunan First Normal University, Changsha 410205, China 2. Foreign Languages Department, Qiannan Normal University for Nationalities, Duyun 558000, China) | 2012 | 海外英语2012，，3X： | 0 |
| 12 | THE ROLE OF COMPOUND BLOCKAGE OF SODIUM CHANNEL ON NEUROPROTECTIVE EFFECT IN STRESSED RATS显示文摘To understand the mechanism of CA3 pyramidal cell deaths caused by stress and to prevent cells death, the study used male rats aged 9 months, by counting the number of CA3 pyramidal cells in hippocampus by H E staining, it was discovered that the number of pyramidal cells in the sham control group and in lidocaine group was 105 02±28 25, 113 75 respectively and there was significant difference (P<0 05). The compound blockage to Na + channel by injection of 2 5μl of 2% lidocaine and 2 5μl of 2% furosemide into the lateral ventricle reduced the selective death in CA3 caused by stress (P<0 01). By Dark Neuron Staining it indicates that stress specificity can not only damage CA3 pyramidal cells in hippocampus, but degenerative neurons are also found in CA1 and CA2 of hippocampus, cerebellum, and septial. | Zhu Zhongliang,Li HuiDepartment of Obstetrics and Gynecology, First Affiliated Hospital, Xi′an Medical University,Fan Xiaoli,Cui DehuaDepartment of Demyelinating Disease and Aging, National Institute of Neuroscience, Japan,Takashi Tabira Dep | 1999 | Journal of Pharmaceutical Analysis1999，13，2： | 0 |
| 13 | Formation of the thermal-driven boundary jet in an f-plane mesoscale basin显示文摘The paper adopts an f-plane quasi-geostrophic inertial model without linearization to investigate the perturbation temperature, boundary jet and upwelling (downwelling) in an idealized rectangular basin, under the consideration of west side friction layer and heat conservation. There is net heat input on the upper surface and equal quality heat dissipation on the west boundary, and without heat exchange on other boundaries, then the heat is conservation in the whole basin. Results show that there is thermal front due to denseness of the perturbation temperature in the west side boundary, the perturbation pressure and flow field are reversal on the upper layer and bottom layer. On the bottom layer, the west coastal current is northward, and the maximum perturbation pressure center is on the west, however, on the upper layer, the east coastal current is southward, and the maximum perturbation pressure center is on the east. There is strong vertical flow in narrow western boundary layer, and also in the central zone. The effect of different upper thermal forcings is also studied, and it can be concluded that there is always temperature denseness and boundary jet near the west boundary, and the appearance of flow field reversal, but the distribution of vertical flow is rather different. | FENG LiCheng1,2 & CHAO JiPing3,4 1 State Key Laboratory of Numerical Modeling for Atmospheric Science and Geophysical Fluid Dynamics (LASG), Institute of Atmospheric Physics, Chinese Academy of Sciences, Beijing 100029, China 2 Graduate University of the Chinese Academy of Sciences, Beijing 100049, China 3 National Marine Environment Forecast Center, Beijing 100081, China 4 First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China | 2008 | Science China Earth Sciences2008，51，1： | 0 |
| 14 | STUDY ON THE STANDARDIZED APPLICATION OF ACUPUNCTURE ANESTHESIA TO CRANIOCEREBRAL OPERATIONS ON TEMPORO-PARIETO-OCCIPITAL REGION AND POSTERIOR CRANIAL FOSSA显示文摘The effect of acupuncture anesthesia(AA) performed with unified protocol andmethod was evaluated by the National Unified Scaling Criteria. Among the 536 cases studied,there are 488 cases Grade I, the rate of Grade I (success rate) being 91.05%. Of the 357 casesof temporo-parieto-occipital operations, the success rate is 91.88%, with the success rates of bodyneedling and ear needling groups being 88.16% and 98.45% respectively. Of the 179 cases of po-sterior cranial fossa operations, the success rate is 89.38%, with the success rates of body needlingand ear needling groups being 85.58% and 95.59% respectively. Randomized controlled trials wereconducted among the simple 0.1% lidocaine group, AA plus 0.1% lidocaine group and AA plusnormal saline group, the results of which prove that acupuncture analgesia plays predominant rolein AA and adjuvants can remarkably enhance the AA effect. The neuroanatomical basis of thesuperiority of ear needling to body needling is discussed here, so is the mechanism of the poten-tiating effect of the adjuvants on AA. Besides the advantages of AA in neurosurgery and problemsremaining unsolved have been summarized. | Jiang Chenchuan1 Li Deze2 Fang Minming3 Luo Qizhong4 Chen Mingzhen5 Ying Shida6 Sung Dajing4 Zhu Hechen1 Xiao Hongrui3 Ma Lie7, National Coordinated Research Group for Acupuncture Anesthesia in Neurosurgery 1. Hua Shan Hospital Affiliated to Shanghai Medical University, Shanghai, China2. Beijing Tiantan Hospital, Beijing, China3. Beijing Xuanwu Hospital, Beijing, China4. Renji Hospital Affiliated to Shanghai Second Medical University. Shanghai. China5. The First Affiliated Hospital of Zhong Shan Medical University, Guangzhou, China6. Affiliated Hospital of Shandong Medical University, Jinan, Shandong, China7. The Second Affiliated Hospital of Zhejiang Medical University, Hangzhou, Zheiiang, China | 1992 | World Journal of Acupuncture-Moxibustion1992，2，1： | 0 |
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