| 5 | 脂多糖和干扰素-γ拮抗吗啡,anandamide,和雌激素诱导的人心房内皮iNOS的表达(英文)显示文摘AIM: To determine whether inducible nitric oxide syn-thase (iNOS) stimulation of human atrial fragments can be diminished by the naturally occurring signal molecules, such as morphine, anandamide, and estrogen . The use of iNOS as an indicator is justified since it has been associated with initiation of various types of cellular damage either directly or indirectly. METHODS: Western blots were performed on control and drug-exposed atrial tissue before and after lipopolysaccharide (LPS) and interferon-γ (IFN-γ) exposure. RESULTS: Preincubation of the tissue with morphine, anandamide or estrogen prior to, but not after, the addition of LPS + IFN-γ, blocked iNOS expression. The nitric oxide donor SNAP also blocked iNOS induction while preincubation of atrial fragments with an inhibitor of NOS, L-NAME, prior to morphine or anandamide exposure, restored LPS + IFN-γ induction of iNOS. CONCLUSION : These data suggest a direct regulatory link at the transcriptional level between constitutive (c) NOS and | Caterina FIMIANI Harold MAGAZINE Ingeborg D WELTERS Thomas V BILFINGER Felice SAL-SANO Else TONNESEN George B STEFANO | 2000 | Acta Pharmacologica Sinica2000，21，5： | 0 |
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