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您的检索式：作者名="Fengning Yu"
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| 1 | Cytosine methylation of the FWA promoter promotes direct in vitro shoot regeneration in Arabidopsis thaliana显示文摘Epigenetic modifications within promoter sequences can act as regulators of gene expression.Shoot regeneration is influenced by both DNA methylation and histone methylation,but the mechanistic basis of this regulation is obscure.Here,we identified 218 genes related to the regeneration capacity of callus that were differentially transcribed between regenerable calli(RC)and non-regenerable calli(NRC)in Arabidopsis thaliana.An analysis of the promoters of five of the differentially expressed genes(FWA,ACC1,TFL1,MAX3,and GRP3)pointed to an inverse relationship between cytosine methylation and transcription.The FWA promoter was demethylated and highly expressed in NRC,whereas it was methylated and expressed at low levels in RC.Explants of the hypomethylation mutants fwa-1 and fwa-2 showed strong levels of FWA expression and regenerated less readily than the wild type,suggesting that FWA inhibits direct in vitro shoot regeneration.WUSCHEL-RELATED HOMEOBOX 9(WOX9),which is required for shoot apical meristem formation,was directly repressed by FWA.Overexpressing WOX9 partly rescued the shoot regeneration defect of fwa-2 plants.These findings suggest that cytosine methylation of the FWA promoter forms part of the regulatory system governing callus regenerability and direct in vitro shoot regeneration. | Xuehuan Dai Jing Wang Yuguang Song Zhenhua Liu Tao Xue Meng Qiao Yanchong Yu Wei Xin Fengning Xiang | 2021 | Journal of Integrative Plant Biology2021，63，8： | 3 |
| 2 | Analysis of diffusion behavior of harmful emissions from trackless rubber-wheel diesel vehicles in underground coal mines显示文摘To define the diffusion behavior of harmful exhaust substances from diesel vehicles and support safety risk assessments of underground coal mines,we performed a multi-species coupling calculation of the emission and diffusion of harmful substances from a trackless rubber-wheel diesel vehicle.A computational fluid dynamics(CFD)model of the diffusion of harmful emissions was hence established and verified.From the perspective of risk analysis,the diffusion behavior and distribution of hazardous substances emitted by the diesel vehicle were studied under 4 different conditions;moreover,we identified areas characterized by hazardous levels of emissions.When the vehicle idled upwind in the roadway,high-risk areas formed behind and to the right of the vehicle:particularly high concentrations of pollutants were measured near the rear floor of the vehicle and within 5 m behind the vehicle.When the vehicle idled downwind,high-risk areas formed in front of it:particularly high concentrations of pollutants were measured near the floor and within 5 m from the front of the vehicle.In the above cases,the driver would not breathe highly polluted air and would be relatively safe.When the vehicle idled into the chamber,however,high-risk areas formed on both sides of the vehicle and near the upper roof.Forward entry of the vehicle caused a greater increase in the concentration of pollutants in the chamber and in the driver’s breathing zone compared with reverse entry. | Yun Hua Wen Nie Qiang Liu Xiaofei Liu Chengyi Liu Weiwei Zhou Fengning Yu | 2022 | International Journal of Mining Science and Technology2022，32，6： | 1 |
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