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| 1 | Dietary garcinol supplementation improves diarrhea and intestinal barrier function associated with its modulation of gut microbiota in weaned piglets显示文摘Background:The effects of dietary garcinol on diarrhea and intestinal barrier function associated with its modulation of gut microbiota in weaned piglets were investigated.Method:One hundred forty four weaned piglets(Duroc×Yorkshire×Landrace)from 16 pens(9 piglets per pen)were randomly divided into four treatment groups:controls(CON)or those supplemented with 200 mg/kg(LOW),400 mg/kg(MID),or 600 mg/kg(HIGH)diet garcinol.After 14-day trial,three piglets per pen were chosen to collect plasma,intestinal tissue and colonic digesta samples.Results:We demonstrated for the first time that garcinol promoted growth performance,as increased average daily feed intake(ADFI)and decreased feed/gain ratio(F/G);and reduced diarrhea incidence(P<0.05);and strengthened antioxidant capacity,as an increased antioxidative index(P<0.05).Additionally,garcinol ameliorated intestinal barrier dysfunction,as an increased villus height to crypt depth ratio,increased zonula occludens protein 1(ZO-1),occludin and claudin-1 expression in the jejunum and ileum(P<0.05),and decreased intestinal permeability(P<0.05);and reduced inflammation,as decreased cytokine interleukin(IL)-6,IL-10,IL-1βand tumor necrosis factor-α(TNF-α)levels in the mucosa of the jejunum and ileum,and NF-κB p65 translocation(P<0.05).Moreover,garcinol inhibited the growth of most harmful bacteria in the gut,especially Escherichia coli,and increased the growth of the beneficial bacteria Lactobacillus.Conclusion:This work provides a fundamental basis for the future development of garcinol-functional food use for improving diarrhea and intestinal barrier function in weaned piglets and for understanding the biological effects of garcinol and its potential as a functional feed additive. | Tongxin Wang Weilei Yao Juan Li Yafei Shao Qiongyu He Jun Xia Feiruo Huang | 2020 | Journal of Animal Science and Biotechnology2020，11，3： | 8 |
| 2 | Transcriptional response of porcine skeletal muscle to feeding a linseed-enriched diet to growing pigs显示文摘Background: To investigate the effect of feeding a linseed-enriched diet to growing-finishing pigs on gene expression in skeletal muscle, pigs were fed with a linseed-enriched diet for 0, 30, 60 and 90 d. Transcriptional profiles of longissimus dorsi muscle were measured using Affymetrix Genechip.Results: Results showed that 264 genes were identified as differentially expressed genes(DEGs). The strongest transcriptional response was clearly observed at 30 d. DEGs were assigned to several main functional terms,including transcription, apoptosis, intracellular receptor-mediated signaling, muscle organ development, fatty acid metabolic process, cell motion, regulation of glucose metabolic process, spermatogenesis and regulation of myeloid cell differentiation. We also found that transcriptional changs of several transcription cofactors might contribute to n-3 PUFAs regulated gene expression. In addition, the increased expression of IGF-1, insulin signaling pathway and the metabolism of amino acids might involve in the muscle growth induced by feeding a linseedenriched diet. The results also provide the new evidence that the expression changes of PTPN1, HK2 and PGC-1αmight contribute to the regulation of insulin sensitivity by n-3 PUFAs.Conclusions: Our finding provided correlative evidence that feeding the linseed enriched diet affact expression of genes involved in insulin signaling pathway and the metabolism of amino acids. | Hongkui Wei Yuanfei Zhou Shuzhong Jiang Feiruo Huang Jian Peng Siwen Jiang | 2016 | Journal of Animal Science and Biotechnology2016，7，2： | 2 |
| 3 | L-leucine stimulates glutamate dehydrogenase activity and glutamate synthesis by regulating mTORC1/SIRT4 pathway in pig liver显示文摘The liver is the most essential organ for the metabolism of ammonia, in where most of ammonia is removed by urea and glutamine synthesis. Regulated by leucine, glutamate dehydrogenase(GDH) catalyzes the reversible inter-conversion of glutamate to ammonia. To determine the mechanism of leucine regulating GDH, pigs weighing 20 ± 1 kg were infused for 80 min with ammonium chloride or alanine in the presence or absence of leucine. Primary pig hepatocytes were incubated with or without leucine. In the in vivo experiments with either ammonium or alanine as the nitrogen source, addition of leucine significantly inhibited ureagenesis and promoted the production of glutamate and glutamine in the perfused pig liver(P < 0.05). Similarly, leucine stimulated GDH activity and inhibited sirtuin4(SIRT4)gene expression(P < 0.01). Leucine could also activate mammalian target of rapamycin complex 1(m TORC1) signaling(P < 0.05), as evidenced by the increased phosphorylation levels of ribosomal protein S6 kinase 1(S6 K1) and ribosomal protein S6(S6). Interestingly, the leucine-induced m TORC1 pathway activation suitably correlated with increased GDH activity and decreased expression of SIRT4.Similar results were observed in primary cultured hepatocytes. Notably, leucine exerted no significant change in GDH activity in SIRT4-deficient hepatocytes(P > 0.05), while m TORC1 signaling was activated.Leucine exerted no significant changes in both GDH activity and SIRT4 gene expression in rapamycin treated hepatocytes(P > 0.05). In conclusion, L-leucine increases GDH activity and stimulates glutamate synthesis from different nitrogen sources by regulating m TORC1/SIRT4 pathway in the liver of pigs. | Tongxin Wang Weilei Yao Qiongyu He Yafei Shao Ruilong Zheng Feiruo Huang | 2018 | Animal Nutrition2018，，3： | 1 |
| 4 | Embelin alleviates weaned piglets intestinal inflammation and barrier dysfunction via PCAF/NF-κB signaling pathway in intestinal epithelial cells显示文摘Background:Intestinal barrier plays key roles in maintaining intestinal homeostasis.Inflammation damage can severely destroy the intestinal integrity of mammals.This study was conducted to investigate the protective effects of embelin and its molecular mechanisms on intestinal inflammation in a porcine model.One hundred sixty 21-day-old castrated weaned pigs(Duroc×Landrace×Yorkshire,average initial body weight was 7.05 d to four groups and fed with a basal diet o±0.28 kg,equal numbers of castrated males and females)were allotter a basal diet containing 200,400,or 600 mg embelin/kg for 28 d.The growth performance,intestinal inflammatory cytokines,morphology of jejunum and ileum,tight junctions in the intestinal mucosa of piglets were tested.IPEC-1 cells with overexpression of P300/CBP associating factor(PCAF)were treated with embelin,the activity of PCAF and acetylation of nuclear factor-κB(NF-κB)were analyzed to determine the effect of embelin on PCAF/NF-κB pathway in vitro.Results:The results showed that embelin decreased(P<0.05)serum D-lactate and diamine oxidase(DAO)levels,and enhanced the expression of ZO-1,occludin and claudin-1 protein in jejunum and ileum.Moreover,the expression levels of critical inflammation molecules(interleukin-1β,interleukin-6,tumor necrosis factor-α,and NF-κB)were downregulated(P<0.05)by embelin in jejunal and ileal mucosa.Meanwhile,the activity of PCAF were down-regulated(P<0.05)by embelin.Importantly,transfection of PCAF siRNAs to IPEC-1 cell decreased NF-κB activities;embelin treatment downregulated(P<0.05)the acetylation and activities of NF-κB by 31.7%-74.6%in IPEC-1 cells with overexpression of PCAF.Conclusions:These results suggested that embelin ameliorates intestinal inflammation in weaned pigs,which might be mediated by suppressing the PCAF/NF-κB signaling pathway. | Weilei Yao Tongxin Wang Lu Huang Zhengxi Bao Shu Wen Feiruo Huang | 2023 | Journal of Animal Science and Biotechnology2023，14，4： | 0 |
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