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| 1 | Cancer stem cells in colorectal cancer from pathogenesis to therapy: Controversies and perspectives显示文摘Colorectal cancer remains one of the most common and lethal malignancies worldwide despite the use of various therapeutic strategies.A better understanding of the mechanisms responsible for tumor initiation and progression is essential for the development of novel,more powerful therapies.The traditional,so-called'stochastic model'of tumor development,which assumes that each cancer cell is tumorigenic,has been deeply challenged during the past decade by the identification of cancer stem cells(CSCs),a biologically distinct subset of cells within the bulk of tumor mass.This discovery led to the development of the hierarchical model of tumorigenesis which assumes that only CSCs have the ability to initiate tumor growth,both at primary and metastatic sites.This model implies that the elimination of all CSCs is fundamental to eradicate tumors and that failure to do so might be responsible for the occurrence of relapses and/or metastases frequently observed in the clinical management of colorectal cancer patients.Identification and isolation of CSCs is essential for a better understanding of their role in the tumorigenetic process and for the development of CSC-specific therapies.Several methods have been used for this purpose and many efforts have been focused on the identification of specific CSC-surface markers.This review provides an overview of the proposed roles of CSC in human colorectal tumorigenesis focusing on the most important molecules identified as CSC-specific markers in colorectal cancer and on the potential strategies for the development of CSC-targeted therapy. | Caterina Fanali Donatella Lucchetti Marisa Farina Maddalena Corbi Valerio Cufino Achille Cittadini Alessandro Sgambato | 2014 | World Journal of Gastroenterology2014，20，4： | 7 |
| 2 | Dispersive liquid-liquid microextraction,an effective tool for the determination of synthetic cannabinoids in oral fluid by liquid chromatography-tandem mass spectrometry显示文摘In the present work,dispersive liquid-liquid microextraction(DLLME)was used to extract six synthetic cannabinoids(JWH-018,JWH-019,JWH-073,JWH-200,or WIN 55,225,JWH-250,and AM-694)from oral fluids.A rapid baseline separation of the analytes was achieved on a bidentate octadecyl silica hydride phase(Cogent Bidentate C18;4.6 mm×250 mm,4μm)maintained at 37℃,by eluting in isocratic conditions(water:acetonitrile(25:75,V/V)).Detection was performed using positive electrospray ionization-tandem mass spectrometry.The parameters affecting DLLME(pH and ionic strength of the aqueous phase,type and volume of the extractant and dispersive solvent,vortex and centrifugation time)were optimized for maximizing yields.In particular,using 0.5 mL of oral fluid,acetonitrile(1 mL),was identified as the best option,both as a solvent to precipitate proteins and as a dispersing solvent in the DLLME procedure.To select an extraction solvent,a low transition temperature mixture(LTTM;composed of sesamol and chlorine chloride with a molar ratio of 1:3)and dichloromethane were compared;the latter(100μL)was proved to be a better extractant,with recoveries ranging from 73%to 101%by vortexing for 2 min.The method was validated according to the guidelines of Food and Drug Administration bioanalytical methods:intra-day and inter-day precisions ranged between 4%and 18%depending on the spike level and analyte;limits of detection spanned from 2 to 18 ng/mL;matrixmatched calibration curves were characterized by determination coefficients greater than 0.9914.Finally,the extraction procedure was compared with previous methods and with innovative techniques,presenting superior reliability,rapidity,simplicity,inexpensiveness,and efficiency. | Pierpaolo Tomai Alessandra Gentili Roberta Curini Rossella Gottardo Franco Tagliaro Salvatore Fanali | 2021 | Journal of Pharmaceutical Analysis2021，11，3： | 4 |
| 3 | Allosteric and binding properties of Asp1-Glu382 truncated recombinant human serum albumin-an optical and NMR spectroscopic investigation显示文摘 | Fanali G Pariani G Ascenzi P | 2009 | FEBS J2009，276，8： | 1 |
| 4 | Controlling enantioselectivity in chiral capillary electrphoresis with inclusion-complexation显示文摘 | | 1997 | J Chromatogr A1997，792，： | 1 |
| 5 | Dental implants placement in conjunction with osteotome sinus floor elevation: a 12-year lifetable analysis from a prospective study on 588 ITI implants 显示文摘 | Ferrigno N Laureti M Fanali S | 2006 | Clin Oral implants Res2006，17，2： | 1 |
| 6 | Chiral analysis by capillary electrophoresis using antibiotics as chiral selector显示文摘 | Desiderio C Fanali A | 1998 | J Chromatogr A1998，807，： | 1 |
| 7 | 显示文摘 | Fanali S Flieger M Sreinerova N | 1992 | Electrophoresis1992，131，1： | 1 |
| 8 | Acute respiratory dis-tress syndrome : update on the latest delopraents in thebasic and cinical research 显示文摘 | Bosma K Fanali V Ranieri V M | 2005 | Cur Opin Anaesthes2005，18，2： | 1 |
| 9 | Analysis of polyphenols and methylxantines in tea samples by means of nano-liquid chromatography utilizing capil ary columns packed with core-shel particles显示文摘 | Fanali C Rocco A Aturki Z | | 0，，20： | 1 |
| 10 | 显示文摘 | DANKOVA M KANIANSKY D FANALI S | 1999 | J Chromatogr: A1999，838，： | 1 |
| 11 | Quantitation of chiral amino acids from microalgae by MEKC and LIF detection 显示文摘 | HERRERO M IBANEZ E FANALI S | 2007 | Electrophores2007，28，： | 1 |
| 12 | Enantioselective determination by capillary electrophoresis with cyclodextrins as chiral selectors 显示文摘 | FANALI S | 2000 | Journal of Cbromatography A2000，875，12： | 1 |
| 13 | Capillary zone electrophoresis of rare earth metals with indirect UV absorbance detection显示文摘 | F Foret S Fanali A Nardi | 1990 | Electrophoresis1990，11，： | 1 |
| 14 | Identification of chiral drug isomers by capillory electrophoresis显示文摘 | Fanali S | 1996 | J Chromatogr A1996，735，12： | 1 |
| 15 | Use of Cyclodextrins for the Enantiosective Separation of Ergot Alkaloids by Capillary Zone Electrophoresis 显示文摘 | Fanali S Flieger M Steinerova N | 1992 | Electrophoresis1992，131，1： | 1 |
| 16 | Dental implants placement in conjunction with osteotome sinus floor elevation: a 12-year lifetable analysis from a prospective study on 588 implants 显示文摘 | Ferrigno N Laureti M Fanali S | 2006 | Clin Oral Impl Res2006，17，： | 1 |
| 17 | Separation of tocopherols by nano-liquid chromatography显示文摘 | FANALI S CAMERA E CHANKVETADZE B | 2004 | Journal of Pharmaceutical and Biomedical Analysis2004，35，： | 1 |
| 18 | Human serum albumin;From bench to bedside显示文摘 | Fanali G di Masi A Trezza V | 2012 | Molecular Aspects of Medicine2012，33，3： | 1 |
| 19 | Inferior alveolar nerve transposition in conjunction with implant placement 显示文摘 | Ferrigno N Laureti M Fanali S | 2005 | Int J Oral Maxillofac Implants2005，20，4： | 1 |
| 20 | Dental implants placement in conjunction with osteotome sinus floor elevation: a 12-year life-table analysis from a prospective stu- dy on 588 ITI implants显示文摘 | Ferrigno N Laureti M Fanali S | 2006 | Clin Oral Im-plants Res2006，17，2： | 1 |
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