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| 1 | Cementomimetics——constructing a cementum-like biomineralized microlayer via amelogenin-derived peptides显示文摘Cementum is the outer-,mineralized-tissue covering the tooth root and an essential part of the system of periodontal tissue that anchors the tooth to the bone. Periodontal disease results from the destructive behavior of the host elicited by an infectious biofilm adhering to the tooth root and left untreated,may lead to tooth loss. We describe a novel protocol for identifying peptide sequences from native proteins with the potential to repair damaged dental tissues by controlling hydroxyapatite biomineralization. Using amelogenin as a case study and a bioinformatics scoring matrix,we identified regions within amelogenin that are shared with a set of hydroxyapatite-binding peptides (HABPs) previously selected by phage display. One 22-amino acid long peptide regions referred to as amelogenin-derived peptide 5 (ADP5) was shown to facilitate cell-free formation of a cementum-like hydroxyapatite mineral layer on demineralized human root dentin that,in turn,supported attachment of periodontal ligament cells in vitro. Our findings have several implications in peptide-assisted mineral formation that mimic biomineralization. By further elaborating the mechanism for protein control over the biomineral formed,we afford new insights into the evolution of protein-mineral interactions. By exploiting small peptide domains of native proteins,our understanding of structure-function relationships of biomineralizing proteins can be extended and these peptides can be utilized to engineer mineral formation. Finally,the cementomimetic layer formed by ADP5 has the potential clinical application to repair diseased root surfaces so as to promote the regeneration of periodontal tissues and thereby reduce the morbidity associated with tooth loss. | Mustafa Gungormus Ersin E Oren Jeremy A Horst Hanson Fong Marketa Hnilova Martha J Somerman Malcolm L Snead Ram Samudrala Candan Tamerler Mehmet Sarikaya | 2012 | International Journal of Oral Science2012，4，2： | 5 |
| 2 | Perioperative outcomes of major hepatic resections under low central venous pressure anesthesia: blood loss, blood transfusion, and the risk of postoperative renal dysfunction显示文摘 | Jose A Melendez Vittoria Arslan Mary E Fischer David Wuest William R Jarnagin Yuman Fong Leslie H Blumgart | 1998 | Journal of the American College of Surgeons1998，，6： | 2 |
| 3 | 肺癌全基因组测序显示文摘肺癌是全球肿瘤发病率和病死率的主因,且预后很差。加强对肿瘤生物学的认识对肺癌研究至关重要。被誉为'下一代测序技术'的NGS技术(next-generation sequencing)是一种针对全基因组鉴定的有力工具,可以对致癌体细胞突变进行全面检测。大多数的NGS技术是基于平台特异性DNA文库进行多重聚合酶链反应(polymerase chain reaction,PCR),从而对目的基因扩增后测序。这种技术适用于高通量测序,可以检测出肿瘤中出现的全部基因组变异。缺点是这种技术需要在时间、实验设备、计算机数据分析、生物信息技术等各方面的大量投入。NGS技术已广泛应用于全基因组、外显子组、转录组和表观基因组的研究中,为肺癌研究和医疗模式带来改变。这项新技术的开展将转变当前对致癌信号通路的认识,可为癌症诊疗提供新的分子靶点。肺癌体细胞突变已有NGS技术的分析报道,但大规模基因组研究仍在进行中。个体化治疗策略将改善那些潜在获益患者的治疗方式,避免'无辜'患者受无效治疗带来的高额费用和不良反应。NGS的组织化、计算机化和生物信息化技术推动了科技的进步,同时,患者知情权和数据发布的相关伦理问题也浮现出来。信号通路中,驱动基因(driver gene)突变和传递基因(passenger gene)突变的区别,需要对测序结果进行细致解读。解读准确与否取决于DNA提取的样本类型、样本处理技术和样本含量。肿瘤异质性也会降低肿瘤基因突变的检测效能。NGS技术将推动对肿瘤基因突变的基础和临床研究,而且,也可应用于单细胞和游离的循环DNA,未来还将用于从体液和肿瘤亚群中获取的DNA样本。如果能进一步降低费用、提高检验速度和精度,NGS技术无疑将会成为肺癌研究的绝佳选择。 | Marissa Daniels Felicia Goh Casey M. Wright Krishna B. Sriram Vandana Relan Belinda E. Clarke Edwina E. Duhig Rayleen V. Bowman Ian A Yang Kwun M. Fong 孙岚 何建行 | 2013 | 国际病理科学与临床杂志2013，33，4： | 2 |
| 4 | Blastocyst transfer after enzymatic treatment of the zona pellucide:improving in vitro fertilization and understanding implantation显示文摘 | Bongso A Ng SC | 1998 | Hum Reprod1998，13，10： | 1 |
| 5 | Cancer prevention and therapy in a preclinical mouse model: impact of FHIT viruses显示文摘 | Ishii H Vecchione A Fong LY | 2004 | Curr Gcnc Thcr2004，4，1： | 1 |
| 6 | Molecular and expression analysis of cowpea trypsin inhibitor (CpTI) gene in transgenic Elaeis guineensis Jacq leaves显示文摘 | Ismanizan I Fong S L Ruslan A | 2010 | Aust J Crop Sci2010，4，： | 1 |
| 7 | Relative CEO underpayment and CEO behav- ior towards R&D spending 显示文摘 | Fong E A | 2010 | Journal of Management Studies2010，47，6： | 1 |
| 8 | Insulin-like Growth Factor 1 Promotes Growth Selectively in fetal sheep in late gestation 显示文摘 | FONG L JULIE A OWENS L | 1996 | AmJPhysiol1996，270，： | 1 |
| 9 | Safety and antitumor activity of recombinant soluble Apo2 ligand 显示文摘 | Ashkenazi A Pal RC Fong S | 1999 | J Ciin Invest1999，104，： | 1 |
| 10 | Prevalence of colorectal polyps in pediatric colonoscopy显示文摘 | Thakkar K Alsarraj A Fong E et al | 2012 | Dig Dis Sci2012，57，4： | 1 |
| 11 | Fungicidal effect of am- photericin B in urine: In vitro study to assess feasibility of bladder wash-out for localization of site of candidacies显示文摘 | Fong I W Cheng P C Hinton N A | 2012 | Ani- microbial Agents and Chemotherapy2012，35，9： | 1 |
| 12 | Pharmacokinetics oftrimethoprim-sulfamethoxazole in critically ill and non-critically ill AIDS patients显示文摘 | Chin T W Vandenbroucke A Fong I W | 1995 | Antimicrob Agents Chemother1995，39，1： | 1 |
| 13 | Ahered peptide ligand vaccination with Fh3 ligand expanded dendritic cells for tumor immunotherapy 显示文摘 | Fong L Hou Y Rivas A | 2001 | Proc Natl Acad Sci U S A2001，98，16： | 1 |
| 14 | The growth of inner cell mass from humanblastoeysts显示文摘 | BONGSO A FONG C Y NG S C | 1994 | Theriogenology1994，41，16： | 1 |
| 15 | Curcumin inhibits the side population (SP) phenotype of the rat C6 glioma cell line: Towards targeting of cancer stem cells with phytochemicals显示文摘 | FONG D YEH A NAFTALOVICH R | 2010 | Cancer Letters2010，293，1： | 1 |
| 16 | Hemihypertrophy显示文摘 | LEUNG A K FONG J H LEONG A G | 2002 | J R Soc Health2002，122，1： | 1 |
| 17 | Bending Instability of Electrically Charged Liquid Jets of Polymer Solution in Electrospinning显示文摘 | RENEKER D H YARIN A L FONG H | 2002 | J Appl Phys2002，587，： | 1 |
| 18 | Aberrant promoter methylation of multiple genes in non-small cell lung cancers显示文摘 | ZOCHBAUER-MULLE S FONG K M VIRMANI A K | 2001 | Cancer Res2001，61，1： | 1 |
| 19 | Altered peptide ligand vaccination with Flt3 ligand expanded dendritic cells for tumor immunotherapy 显示文摘 | FONG L HOU Y RIVAS A | 2001 | Proc Natl Acad Sci USA2001，98，15： | 1 |
| 20 | Rice by-products: comparative extraction and precipitation of nitrogen from us and spanish bran and germ显示文摘 | Betschart A A Fong R Y Saunders R M | 1977 | Journal of Food Science1977，42，4： | 1 |
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