| 1 | Comparison of three PCR methods for detection of Helicobacter pylori DNA and detection of cagA gene in gastric biopsy specimens显示文摘AIM: To comparatively evaluate PCR and other diagnostic methods (the rapid urease test and / or culture) in order to determine which of the three PCR methods (ureA, glmM and 26-kDa, SSA gene) was most appropriate in the diagnosis of Helicobacterpylon (Hpylo#) infection and also to evaluate the detection of a putative virulence marker of Hpylori,the cagA. gene, by PCR in biopsy specimens.METHODS: One hundred and eighty-nine biopsy specimens were collected from 63 patients (three biopsies each) undergoing upper gastroduodenal endoscopy for various dyspeptic symptoms. The PCR methods used to detect HpylonDNA directly from biopsies were the glmM, 26-kDa,ureA and then cagA was used to compare the culturet echnique and CLO for urease with the culture technique being used as the gold standard.RESULTS: Thirty-five percent of the biopsies were positive for Hpy/or/DNA using the 3 PCR methods, while 68% of these were positive for the cagA gene. Twenty-four percent of the biopsies were negative for Hpy/or/DNA in all PCR methods screened. The remaining 41% were either positive for urea gene only, g/mMonly, 26-kDa only, or urea + g/mM,urea + 26-kDa, glmM + 26-kDa. Out of the 35% positive biopsies, 41% and 82% were positive by culture and CLO respectively, while all negative biopsies were also negative by culture and cagA. Cag A+ infection was also predominantly found in H pylori DNA of the biopsies irrespective of the clinical diagnosis.CONCLUSION: This method is useful for correctly identifying infections caused by Hpy/or/and can be easily applied in our laboratory for diagnostic purposes. | SI Smith KS Oyedeji AO Arigbabu FCantet FMegraud OOOjo AOUwaifo JAOtegbayo SOOla AO Coker | 2004 | World Journal of Gastroenterology2004，10，13： | 5 |
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