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| 1 | Genetic analysis of 'all-fish' growth hormone gene transferred carp (Cyprinus carpio L.) and its F_1 generation显示文摘Recornbinant 'all-fish' growth hormone gene (GH) was microinjected into the fertilized eggs of carp. A comparison between the growth traits of transgenics and non-transgenics was carried out, and the transgenic individuals with significant 'fast-growing' effect were successfully gained. A comparison on the reproductivities was also given out between the transgenics and their non-transgenic siblings, and showed that the reproductive capacity of transgenics was substantially equivalent to those of the non-transgenics. On the other hand, the genetic separation and the characteristic distribution of the FI generation were genetically analyzed, which gave solid evidence for the hypothesis that 2-3 chromosomes are integrated with trans-gene. In addition, the distinct biological effects for multi-site-integrated transgenes were further discussed. The present study opens a door for the breeding of 'fast-growing' transgenic fish. | WANG Yaping, HU Wei, WU Gang,SUN Yonghua, CHEN Shangping, ZHANG Fuying,ZHU Zuoyan, FENG Jianxin & ZHANG Xirui1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China 2. Henan Institute of Aquaculture, Zhengzhou 450044, China | 2001 | Chinese Science Bulletin2001，46，14： | 23 |
| 2 | Proteorhodopsin——A new path for biological utilization of light energy in the sea显示文摘The breakthrough of environmental ge-nomics of marine microbes has revealed the exis-tence of eubacterial rhodopsin in the sea, named proteorhodopsin (PR), which can take light to pro-duce bio-energy for cell metabolism. Gene and protein sequence analysis and laser flash-induced photolysis experiments have validated the function of PR as light-driven proton-pump. During the pumping process, light energy is transformed into chemical gradient potential across plasma inner-membrane, the potential energy is then used to synthesize ATP. The finding of PR actually brings to light a novel pathway of sunlight utilization existing in heterotro-phic eubacteria in contrast to the well-known chloro-phyll-dependent photosynthesis in the sea. Since the group of PR-bearing bacteria is one of the numeri-cally richest microorganisms on the Earth, accounting for 13% of the total in sea surface water, and with averaged cellular PR molecules of 2.5×104, PR- bearing bacteria are a key component not to be ig-nored in energy metabolism and carbon cycling in the sea. Based on the understanding of current literature and our own investigation on PR in the China seas which indicated a ubiquitous presence and high di-versity of PR in all the marine environments, we propose a conceptual model of energy flow and car-bon cycling driven by both pigment-dependent and -independent biological utilization of light in the ocean. | JIAO Nianzhi FENG Fuying WEI Bo | 2006 | Chinese Science Bulletin2006，51，8： | 3 |
| 3 | Chitosan graft copolymer nanoparticles for oral profein drug delivery: Preparation and characterization 显示文摘 | Feng Qian Fuying Cui Jieying Ding | 2006 | Biomacromolecules2006，，7： | 1 |
| 4 | Chitosan Graft Copolymer Nanoparticles for Oral Protein Drug Delivery: Preparation and Characterization显示文摘 | Qian Feng Cui Fuying Ding Jieying | 2006 | Biomacromolecules2006，7，： | 1 |
| 5 | Phylogenetic analysis of aerobic anoxygenic phototrophic bacteria and their relatives based on farnesyl pyrophosphate synthase gene显示文摘The study aims to reveal phylogenetic and evolutionary relationship between aerobic anoxygenic phototrophic bacteria(AAnPB) and their relatives,anaerobic anoxygenic phototrophic bacteria(AnAnPB) and nonphototrophic bacteria(NPB,which had high homology of 16S rDNA gene with AAnPB and fell into the same genus),and validate reliability and usefulness of farnesyl pyrophosphate synthase(FPPS) gene for the phylogenetic determination.FPPS genes with our modified primers and 16S rDNA genes with general primers,were amplified and sequenced or retrieved from GenBank database.In contrast to 16S rDNA gene phylogenetic tree,AAnPB were grouped into two clusters and one branch alone with no intermingling with NPB and AnAnPB in the tree constructed on FPPS.One branch of AAnPB,in both trees,was located closer to outgroup species than AnAnPB,which implicated that some AAnPB would be diverged earlier in FPPS evolutionary history than AnAnPB and NPB.Some AAnPB and NPB were closer located in both trees and this suggested that they were the closer relatives than AnAnPB.Combination codon usage in FPPS with phylogenetic analysis,the results indicates that FPPS gene and 16S rRNA gene have similar evolutionary pattern but the former seems to be more reliable and useful in determining the phylogenic and evolutionary relationship between AAnPB and their relatives.This is the first attempt to use a molecular marker beside 16S rRNA gene for studying the phylogeny of AAnPB,and the study may also be helpful in understanding the evolutionary relationship among phototrophic microbes and the trends of photosynthetic genes transfer. | FENG Fuying JIAO Nianzhi DU Hailian ZENG Yonghui | 2010 | Acta Oceanologica Sinica2010，29，5： | 1 |
| 6 | Two birds with one stone:Amine-functionalized MSNs@Eu(OH)CO_(3) nanoprobe for efficient dissolution-enhanced afterglow bioassay显示文摘ABSTRACT The efficient detection of tumor markers is crucial to early cancer diagnosis and monitoring.Here,we propose a unique“two birds with one stone”dissolution-enhanced afterglow bioassay based on amino-functionalized mesoporous SiO2 nanoparticles(MSNs-NH2)encapsulated Eu(OH)CO_(3)nanoprobes.These nanoprobes(i)stably conjugate to the antibody and(ii)are highly soluble in the acidic enhancer solution,and provide a high Eu3+concentration.The released Eu3+ions further activate the photochemical afterglow system to determine the alpha-fetoprotein(AFP)levels in clinical serum samples.Without the use of a real-time excitation source,the interference of autofluorescence and scattering from blood can be avoided.The demonstrated limit of detection(LOD)for AFP was as low as 0.12 ng·mL^(−1).This covalently connected dissolution-enhanced luminescent bioassay(DELBA)and novel afterglow system shows tremendous potential for ultrasensitive and rapid clinical diagnosis. | Fuying Zhang Donghao Hu Xianlong Su Zhengdao Hong Wei Feng Ming Xu Fuyou Li | 2022 | Nano Research2022，15，9： | 1 |
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