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| 1 | 自身免疫性小脑炎相关抗神经抗体的筛查与临床意义显示文摘目的在病因未明的小脑性共济失调患者中筛查自身免疫性小脑炎(autoimmune cerebellitis,AC)相关抗体并探讨其临床意义。方法收集病因未明的小脑性共济失调患者(来自2016—2018年北京协和医院神经科门诊和住院患者、北京脑炎协作组成员单位提供的病例)的血清和(或)脑脊液,进行AC相关抗神经抗体检测。采用德国欧蒙公司的间接免疫荧光试剂盒、间接免疫荧光转染细胞(基于细胞底物的实验)法和猴小脑组织(基于组织底物的实验)多抗原共片生物马赛克。所检测的AC抗体谱包括抗谷氨酸脱羧酶65(glutamic acid decarboxylase 65,GAD65)、锌指蛋白4(zinc finger protein 4,ZIC4)、Tr[delta/notch样表皮生长因子相关受体(delta/notch-like epidermal growth factor-related receptor,DNER)]、三磷酸肌醇受体1(inositol 1,4,5-trisphosphate receptor 1,ITPR1)、Homer同源蛋白3(homer protein homolog 3,Homer-3)、神经软骨素(neurochondrin,NCDN)、碳酸酐酶相关蛋白Ⅷ(carbonic anhydrase-related protein Ⅷ,CARP Ⅷ)和浦肯野细胞2型(Purkinje cell antibody 2,PCA2)抗体。结果全部400例患者中共8例患者通过该AC抗体谱检测到抗神经抗体(检出比例2%),其中包括抗GAD65抗体阳性2例,抗Tr(DNER)抗体阳性2例,抗PCA-2抗体阳性1例,抗Homer-3抗体阳性1例,抗NCDN抗体阳性2例。8例患者均临床主要表现为小脑性共济失调,部分患者合并边缘性脑炎,1例抗GAD65抗体阳性患者合并小细胞肺癌,8例患者接受免疫治疗,4例患者部分有效。结论自身免疫性小脑共济失调是获得性小脑性共济失调的病因之一,AC抗体谱的检测有助于该病的诊断。新AC抗体谱阳性患者多数为非副肿瘤性AC,部分病例免疫治疗有一定效果。 | 任海涛 徐晓璐 关鸿志 范思远 钱敏 杨洵哲 李力波 马明圣 邸卫英 张炜华 Fechner Kai 彭斌 崔丽英 | 2019 | 中华神经科杂志2019，52，4： | 13 |
| 2 | MicroRNA-regulated viral vectors for gene therapy显示文摘Safe and effective gene therapy approaches require targeted tissue-specific transfer of a therapeutic transgene.Besides traditional approaches, such as transcriptional and transductional targeting, micro RNA-dependent posttranscriptional suppression of transgene expression has been emerging as powerful new technology to increase the specificity of vector-mediated transgene expression. Micro RNAs are small non-coding RNAs and often expressed in a tissue-, lineage-, activation- or differentiation-specific pattern. They typically regulate gene expression by binding to imperfectly complementary sequences in the 3' untranslated region(UTR) of the m RNA. To control exogenous transgene expression, tandem repeats of artificial micro RNA target sites are usually incorporated into the 3' UTR of the transgene expression cassette, leading to subsequent degradation of transgene m RNA in cel s expressing the corresponding micro RNA. This targeting strategy, first shown for lentiviral vectors in antigen presenting cells, has now been used for tissue-specific expression of vector-encoded therapeutic transgenes, to reduce immune response against the transgene, to control virus tropism for oncolytic virotherapy, to increase safety of live attenuated virus vaccines and to identify and select cell subsets for pluripotent stem cell therapies, respectively. This review provides an introduction into the technical mechanism underlying micro RNA-regulation, highlights new developments in this field and gives an overview of applications of micro RNA-regulated viral vectors for cardiac, suicide gene cancer and hematopoietic stem cell therapy, as well as for treatment of neurological and eye diseases. | Anja Geisler Henry Fechner | 2016 | World Journal of Experimental Medicine2016，6，2： | 9 |
| 3 | Population Pharmacokinetic Modeling of Hydromorphone in Cardiac Surgery Patients during Postoperative Pain Therapy显示文摘 | Christian Jeleazcov Teijo I. Saari Harald Ihmsen Jan Mell Katharina Fr?hlich Ljubica Krajinovic J?rg Fechner Jürgen Schüttler | 2014 | Anesthesiology2014，，2： | 2 |
| 4 | Correction of myopia by implantation of a concave worst iris claw lens into phakic eyes显示文摘 | Fechner PU Strobel J Wichmann W | 1991 | Refract Corneal Surg1991，7，： | 1 |
| 5 | Induction of coxsackievirus adenovirus receptor expression during myocardial tissue formation and remodeling identification of a cell-to-cell contact-dependent regulatory mechanism 显示文摘 | FECHNER H NOUTSIAS M TSCHOEPE C et al | 2003 | Circulation2003，107，6： | 1 |
| 6 | Herpes simplex keratitis after photorefractive keratectomy 显示文摘 | Wulff K Fechner PU | 1997 | Refract Surg1997，13，7： | 1 |
| 7 | inter-observer reproducibility in the diagnosis of ductal proliferative breast lesions using standardized criteria显示文摘 | Schnitt SJ Connolly JL Tavassoli FA Fechner RE Kempson RL Gelman R Page DL | 1992 | Am J Surg Pathol1992，16，12： | 1 |
| 8 | Efficacy of hyaluronidase显示文摘 | Fechner PU | 2000 | Arch Ophthalmol2000，118，3： | 1 |
| 9 | Poly- ethylenimine-graft-poly ( ethylene glycol) copolymers: influ- ence of copolymer block structure on DNA complexation and biological activities as gene delivery system 显示文摘 | PETERSEN H FECHNER P M MARTIN A L | 2002 | Bioconjugate Chem2002，13，4： | 1 |
| 10 | Graph kernels for chemical compounds using topological and three dimensional local atom pair environments显示文摘 | Hinselmann G Fechner N Jahn A | 2010 | Neurocomputing2010，74，1： | 1 |
| 11 | Postoperative pain man- agement following remifentanil-based anesthesia for major abdomi- nal surgery 显示文摘 | Albrecht S Schuttler J Fechner J | 2011 | Anesth Analg2011，86，6： | 1 |
| 12 | Magne- sium alloys as implant materials:Principles of property design for Mg-RE alloys显示文摘 | HORT N HUANG Y FECHNER D | 2010 | Acta Biomaterialia2010，6，： | 1 |
| 13 | Fabrication of High Aspect Ratio Sub-100 nm Patterns in Fused Silica显示文摘 | J. Zajadacz, R. Fechner K. Zimmer | 2012 | 材料科学与工程(中英文A版)2012，2，5： | 1 |
| 14 | Clinical investigations on the effect of morphine, pentayocine, ethedine, piriyramide and tramadol on respiration显示文摘 | Fechner R | 1985 | Anasth Intensiverned1985，26，： | 1 |
| 15 | Study on the effects of heat on blood and on the postmortem estimation of carboxyhemoglobin and methemoglobin显示文摘 | Fechner GP Gee DJ | 1989 | Forensic Sci Int1989，40，： | 1 |
| 16 | Robotic-assisted laparoscopic cerclage in a pregnant patient显示文摘 | Fechner AJ Alvarez M Smith DH | 2009 | Am J Obstet Gynecol2009，200，： | 1 |
| 17 | The impact of intra-operative sufentanil dosing on post-operative pain,hyperalgesia and morphine consumption after cardiac surgery显示文摘 | Fechner J Ihmsen H Schüttler J | 2013 | Eur J Pain2013，17，4： | 1 |
| 18 | Synthesis, characterization, and biocompatibility of Polyethylenimine-graft Poly (ethylene glycol) block copolymers 显示文摘 | HOLGER PETERSEN PETRA M FECHNER | 2002 | Macromolecules2002，35，： | 1 |
| 19 | Ultra-precision surface finishing by ion beam and plasma jet techniques-status and outlook显示文摘 | T Arnold G Bohm R Fechner | 2010 | Nucl Instrum Methods Phys Res A2010，616，23： | 1 |
| 20 | Completion pneumonectomy:current indications,complications,and results显示文摘 | Fujimoto T Zaboura G Fechner S | 2001 | J ThoracCardiovasc Surg2001，121，3： | 1 |
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