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| 1 | Antidiabetic principles of Loranthus micranthus Linn.parasitic on Persea americana显示文摘Objective:To explore antidiabetic principles of the Eastern Nigeria mistletoe,Loranlhus micranthus Linn,parasitic on Persea Americana.Methods:The weakly acidic fraction of the aqueous methanol extract of the leaves of Loranlhus micranthus(Linn.) was isolated and tested for its antidiabetic activities.The isolation of the weakly acidic fraction was carried out following established physico-chemical based procedures.Furthermore,alloxan-induced diabetic rats were treated intraperitoneally(ip) with 250 mg/kg and 400 mg/kg of the weakly acidic fraction, glibenclamide 10 mg/kg(positive control) and 2 mg/kg of 3%v/v tween 20(negative control).The sugar levels of the treated and untreated animals were determined by withdrawing the blood at regular intervals and testing them with an automated glucometer.The phytochemical analysis of the acidic fraction was carried out using standard procedures.Chromatographic techniques were employed in the subsequent isolation and purification of the constituents of the weakly acidic fraction.Results:It was shown that the maximum effect of the weakly acidic fraction was obtained at 24 hours after administration and was found to be statistically comparable with that of the positive control.The phytochemical analysis revealed the presence of steroids,terpenoids, alkaloids,flavonoids,glycosides,carbohydrates,saponins,and acidic compounds in the crude extract and carbohydrates,flavonoids.terpenoids and oil in the weakly acidic fraction.Further purification of the weakly acidic fraction of the methanol extract using thin layer chromatography shows that toluene:methanol:diethyl amine(3:1:1) and chloroform:methanol:diethyl amine (9:1:1) are the best solvent system for the isolation of the various components of the weakly acidic fraction of the crude methanol extract of Loranlhus micranthus.Conclusions:The present study has led to the conclusion that the weakly acidic fraction of the plant under study has the potent antidiabetic activity and that the various components can be isolated using basic chromatographic techniques. | Patience Ogoamaka Osadebe Edwin Ogechukwu Omeje Sylvester Chukwuemeka Nworu Charles Okechukwu Esimone Philip Felix Uzor Ernest kenechukwu David John Uchechukwu Uzoma | 2010 | Asian Pacific Journal of Tropical Medicine2010，3，8： | 4 |
| 2 | Antibacterial and wound healing properties of methanolic extracts of some Nigerian medicinal plants显示文摘 | K.F. Chah C.A. Eze C.E. Emuelosi C.O. Esimone | 2005 | Journal of Ethnopharmacology2005，，1： | 2 |
| 3 | Immunomudulatory activities of kolaviron, a mixture of three related biflavonoids of Garcinia kola Heckel显示文摘 | Nworu CS Akah PA Esimone CO | 2008 | Immunopharmacol Immunotoxicol2008，30，2： | 1 |
| 4 | Synthesis of fuoropolymers in supercritical carbon dioxide 显示文摘 | )ESIMONE J M GUAN Z ELSBERND C S | | Science0，257，5072： | 1 |
| 5 | Intellectual Property Rights Enforcement in Nigeria: A Prop for Music Industry显示文摘 | Ebele V. Ojukwu Young Sook Onyiuke Chinyere C. Esimone | 2015 | US-China Education Review(B)2015，5，6： | 1 |
| 6 | Phospholipon 90H(P90H)-based PEGylated microscopic lipospheres delivery system for gentamicin:an antibiotic evaluation显示文摘Objective:To formulate gentamicin liposphere by solvent-melting method using lipids and polyethylene glycol 4000(PEG-4000) for oral administration.Methods:Gentamicin lipospheres were prepared by melt-emulsification using 30%w/w Phospholipon(?) 90H in Beeswax as the lipid matrix containing PEC-4000.These lipospheres were characterized by evaluating on encapsulation efficiency,loading capacity,change in pH and the release profile. Antimicrobial activities were evaluated against Escherichia coli,Pseudomonas aeruginosa. Salmonella paratyphii and Staphylococcus aureus using the agar diffusion method.Results: Photomicrographs revealed spherical particles within a micrometer range with minimal growth after 1 month.The release of gentamicin in vitro varied widely with the PEC-4000 contents. Moreover,significant(P>0.05) amount of gentamicin was released in vivo from the formulation. The encapsulation and loading capacity were all high,indicating the ability of the lipids to take up the drug.The antimicrobial activities were very high especially against Pseudomonas compare to other test organisms.This strongly suggested that the formulation retain its bioactive characteristics.Conclusions:This study strongly suggest that the issue of gentamicin stability and poor absorption in oral formulation could be adequately addressed by tactical engineering of lipid drug delivery systems such as lipospheres. | Mumuni Audu Momoh Charles Okechukwu Esimone | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，11： | 1 |
| 7 | Evaluation of extracts of the root of Landolphia owerrience for antibacterial activity显示文摘 | M.I Okeke C.U Iroegbu E.N Eze A.S Okoli C.O Esimone | 2001 | Journal of Ethnopharmacology2001，，2： | 1 |
| 8 | Safety evaluation in mice of the childhood immunization vaccines from two south-eastern states of Nigeria显示文摘Objective:To check the effects of the vaccines on the hematopoietic system and weight of mice after immunization.Methods:The study was done with the Expanded Programme on Immunization vaccines donated by the Ministries of Health of Abia and Imo States of Nigeria.The vaccines were collected from the cold-chain stores and transported in vaccine carriers to the cold-chain facility in Nnamdi Azikiwe University Teaching Hospital within 3 hours of collection.They were used to immunize a total of 160 mice.The Ethics Committee of Nnamdi Azikiwe University Teaching Hospital,Nnewi of Anambra State,Nigeria approved the protocol.Results:Mice body weight changes test showed that the mice all had increased body weight at Days 3 and 7 post-immunization and none died during the 7 d post-immunization observation.The percentage weight gains of the mice compared with the control were 69%.70%,64%.63%,65%and 68%for oral polio vaccine,diphtheria-pertussis-tetanus.bacillus CalmetteGuerin,measles,yellow fever and hepatitis B vaccines respectively collected from Imo State.The mice immunized with oral polio vaccine,pentavalent.bacillus Calmette-Guerin.measles,yellow fever and hepatitis B vaccines collected from Abia State had 123%.114%,121%.116%,142%and 119%weight gain respectively compared with the control.Leukocytosis promoting toxicity test showed that none of the vaccines was able to induce proliferation of leukocytes up to ten folds.Leukopenic toxicity test showed that all the vaccines had an leukopenic toxicity test value higher than 80%of the control(physiological saline).Conclusions:The vaccine samples tested were safe and did not affect the hematopoietic system adversely.The storage conditions of the vaccines in the States' cold-chain stores had not compromised the safety of the vaccines. | Oli Angus Nnamdi Agu Remigus Uchenna Oli Ugochukwu Chinedum Nwoye Charles Ugochukwu Ejiofor Obiora Shedrack Esimone Charles Okechukwu | 2015 | Asian Pacific Journal of Tropical Biomedicine2015，5，2： | 0 |
| 9 | Effects of some common additives on the antimicrobial activities of alcohol-based hand sanitizers显示文摘Objective: To study the effects of some common additives on the antimicrobial activities of alcohol-based hand sanitizers. Methods: The antibacterial activities of varying aqueous concentrations of ethanol and isopropyl alcohol were tested by the agar well diffusion method. The influences of different concentrations of glycerin was similarly tested. Finally, isopropyl alcohol and benzalkonium chloride were combined in different ratios within the sate use concentrations of each, and the effects of these combinations were compared with values obtained for the two agents used alone. Statistical methods, such as student t test and one-way ANOVA were used when appropriate to evaluate the differences in activity. Results: The activities of the alcohols showed marked concentration dependence, and both showed peak activity at 85%-95% concentration range. Over the concentration range of 60%-100%, isopropyl alcohol inhibited more bacterial and fungal organisms than ethanol, though the inhibition zone diameters it produced were not statistically different from those of ethanol for organisms which were sensitive to both of them.Addition of glycerin reduced the antimicrobial activities of the isopropyl alcohol, as shown by reduction in the inhibition zone diameters produced in vitro, which may be due to reduced drug diffusion with increase in viscosity. Addition of benzalkonium to isopropyl alcohol systems improved the activity of the alcohol, but the overall activity of the combination was not superior to that seen in the use of benzalkonium alone. Conclusion: Alcohol-based hand sanitizers should not be used outside the concentration range of 85%-95% and isopropyl alcohol inhibits more bacterial and fungal organisms than ethanol for most concentrations. Inclusion of benzalkonium improves the antimicrobial spectrum and activity of isopropyl alcohol, and the combination may justifiably be used to achieve both immediate and long lasting effect. Glycerin may adversely affect the antimicrobial activities of isopropyl alcohol-based hand sanitizers and should be used with caution. | Nzekwe Ifeanyi Thaddeus Egbuna Chukwuemeka Francis Okpara Ogonna Jane Agubata Chukwuma Obumneme Esimone Charles Okechukwu | 2018 | Asian Pacific Journal of Tropical Medicine2018，11，3： | 0 |
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