|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | An Outbreak of NCIP (2019-nCoV) Infection in China --Wuhan, Hubei Province, 2019−2020显示文摘Emerging and re-emerging pathogens are great challenges to the public health(1).A cluster of cases of viral pneumonia of unknown etiology(VPUE),now known as novel coronavirus-infected pneumonia(NCIP),occurred in Wuhan,Hubei Province and was reported to health authorities on December 29,2019(2).A national,provincial,and municipal joint investigation team has been assembled to conduct field investigations and implement disease control and prevention measures.This report shows interim results of the investigation and makes recommendations for response measures. | The 2019-nCoV Outbreak Joint Field Epidemiology Investigation Team Qun Li | 2020 | China CDC weekly2020，2，5： | 31 |
| 3 | Cytochrome P450 2E1 genetic polymorphism and gastric cancer in Changle,Fujian Province显示文摘AIM: Genetic polymorphism in enzymes of carcinogen metabolism has been found to have the influence on the susceptibility to cancer. Cytochrome P450 2E1 ( CYP2 E1) is considered to play an important role in the metabolic activation of procarcinogens such as N-nitroscoamines and Iow molecular weight organic compounds. The purpose of this study is to determine whether CYP450 2Elpolymorphisms are associated with risk s of gastric cancer.METHODS: We conducted a population based case-control study in Changle county, Fujian Province, a high-risk region of gastric cancer in China. Ninety-one incident gastric cancer patients and ninety-four healthy controls were included in our study. Datas including dsmographic characteristcs, diet intake, and alcohol and tobacco consumption of indivduals in our study were completed by a standardized questionnaire. PCR-RFLP revealed three genotypes: heterozygote (C1/C2) and two homozygotes (C1/C1 and C2/C2) in CYP2E1.RESULTS: The frequency of variant genotypes (C1/C2 and C2/C2) in gastric cancer cases and controls was 36.3% and 24.5%, respectively. The rare homozygous C2/C2 genotype was found in 6 indivduals in gastric cancer group(6.6%),whereas there was only one in the control group (1.1%).However, there was no statistically significan difference between the two groups (two-tailed Fisher′s exact test, P =0.066). Indivduals in gastric cancer group were more likely to carry genotype C1/C2 (odds ratio, OR = 1.50) and C2/C2(OR = 7.34) than indivduals in control group (X2 = 4.597, for trend P=0.032). The frequencies of genofypes with the C2allele ( C1/C2 and C2/C2 genotypes) were compared with those of genotypes without C2 allele ( C1/C1 genotype )among indivduals in gastric cancer group and control group according to the pattern of gastric cancer risk factors. The results show that indivduals who exposed to these gastric cancer risk factors and carry the C2 allele seemed to have a higher risk of developing gastric cancer.CONCLUSION: Polymorphism of CYP2E1 gene may have some effct in the development of gastric cancer in Changle county, Fujian Province. | Lin Cai~1 Shun-Zhang Yu~2 Zuo-Feng Zhang~3 1 Department of Epidemiology,Fujian Medical University,Fuzhou 350004,Fujian Province,China2 Department of Epidemiology,Shanghai Medical University,Shanghai 200032,China3 Department of Epidemiology,UCLA School of Public Health,Los Angeles California,USA | 2001 | World Journal of Gastroenterology2001，7，6： | 25 |
| 4 | Glutathione S-transferases M1,T1 genotypes and the risk of gastric cancer:A case-control study显示文摘AIM Glutathione S-transferases (GSTs are involved in the detoxification of many potential carcinogens and appear to play a critical role in the protection from the effects of carcinogens. The contribution of glutathione Stransferases M1 and T1 genotypes to susceptibility to the risk of gastric cancer and their interaction with cigarette smoking are still unclear. The aim of this study was to determine whether there was any relationship between genetic polymorphisms of GSTM1 and GSTT1 and gastric cancer.METHODS A population based case - control study was carried out in a high-risk area, Changle County, Fujian Province, China. The epidemiological data were collected by a standard questionnaire and blood samples were obtained from 95 incidence gastric cancer cases and 94 healthy controls. A polymerase chain reaction method was used to detect the presence or absence of the GSTM1 and GSTT1 genes in genomic DNA. Logistic regression model was employed in the data analysis.RESULTS An increase in risk for gastric cancer was found among carriers of GSTM1 null genotype. The adjusted odds ratio (OR) was 2.63 [95% Confidence Interval (95% CI) 1.17-5.88], after controlling for age,gender, cigarette smoking, alcohol drinking, and fish sauce intake. The frequency of GSTT1 null genotype in cancer cases (43.16%) was not significantly different from that in controls (50.00%). However, the risk for gastric cancer in those with GSTM1 null and GSTT1 nonnull genotype was significantly higher than in those with both GSTM1 and GSTT1 non-null genotype (OR = 2.77,95% Cl 1.15- 6.77). Compared with those subjects who never smoked and had normal GSTM1 genotype, Ors were 1.60 (95% CI: 0.62- 4.19) for never smokers with GSTM1 null type, 2.33 (95% CI 0.88- 6.28) for smokers with normal GSTM1, and 8.06 (95% CI 2.83- 23.67) for smokers with GSTM1 null type.CONCLUSIONS GSTM1 gene polymorphisms may be associated with genetic susceptibility of stomach cancer and may modulate tobacco-related carcinogenesis of gastric cancer. | Lin Cai Shun-Zhang Yu Zuo-Feng Zhang Department of Epidemiology.Fujian Medical University,Fuzhou 350004,Fujian Province,ChinaDepartment of Epidemiology,Shanghai Medical University,Shanghai 200032,China Department of Epidemiology,UCLA School of Public Health,Los Angeles California,USA | 2001 | World Journal of Gastroenterology2001，7，4： | 22 |
| 5 | Population-based study of DNA image cytometry as screening method for esophageal cancer显示文摘AIM:To explore the DNA image cytometry (DNA-ICM) technique as a primary screening method for esopha-geal squamous precancerous lesions.METHODS:This study was designed as a population-based screening study.A total of 582 local residents aged 40 years-69 years were recruited from Linzhou in Henan and Feicheng in Shandong.However,only 452 subjects had results of liquid-based cytology,DNA-ICM and pathology.The sensitivity and specificity of DNA-ICM were calculated and compared with liquid-based cytology in moderate dysplasia or worse.RESULTS:Sensitivities of DNA-ICM ranging from at least 1 to 4 aneuploid cells were 90.91%,86.36%,79.55% and 77.27%,respectively,which were better than that of liquid-based cytology (75%).Specifici-ties of DNA-ICM were 70.83%,84.07%,92.65% and 96.81%,but the specificity of liquid-based cytology was 91.91%.The sensitivity and specificity of a combination of liquid-based cytology and DNA-ICM were 84.09% and 85.78%,respectively.CONCLUSION:It is possible to use DNA-ICM tech-nique as a primary screening method for esophageal squamous precancerous lesions. | Lin Zhao,Wen-Qiang Wei,Xin-Qing Li,Guo-Qing Wang,Qi Shang,You-Lin Qiao,Department of Cancer Epidemiology,Cancer Institute/Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100021,China De-Li Zhao,Department of Epidemiology,Feicheng People’s Hospital,Feicheng 271600,Shandong Province,China Chang-Qing Hao,Department of Endoscopy,Linzhou Cancer Hospital,Linzhou 456500,Henan Province,China Dong-Mei Lin,Qin-Jing Pan,Department of Pathology,Cancer Institute/Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100021,China Fu-Hua Lei,Department of Pathology,Feicheng People’s Hospi-tal,Feicheng 271600,Shandong Province,China Jin-Wu Wang,Department of Pathology,Linzhou Cancer Hospi-tal,Linzhou 456500,Henan Province,China | 2012 | World Journal of Gastroenterology2012，18，4： | 15 |
| 6 | Susceptibility to hepatocellular carcinoma associated with null genotypes of GSTM] and GSTT1显示文摘AIM In order to study the association betweenthe null genotypes of GSTM1 and GSTT1 and thegenetic susceptibility to hepatocellularcarcinoma(HCC).METHODS The genotypes of GSTM1 and GSTT1of 63 cases of HCC and 88 controls were detectedwith the multiple PCR technique.RESULTS The frequency of GSTM1 nullgenotype was 57.1% among the cases,and42.0% among the controls,the difference beingstatistically significant(χ~2=3.35,P=0.067),but χ~2 value approaching the significance level.The odds ratio was 1.84(95% Cl= 0.91-3.37).The frequency of GSTT1 non-null genotype was87.3% among the cases and 52.5% among thecontrols,the difference being statisticallysignificant(χ~2=11.42,P=0.0007274).The oddsratio was 4.13(95% Cl=1.64-10.70).According to the cross analysis,the GSTT1 non-null genotype was more closely associated withHCC than GSTM1 null genotype,and these twofactors play an approximate additive interactionin the occurrence of HCC.CONCLUSION The persons with GSTM1 nullgenotype and GSTT1 non-null genotype have theincreased risk to HCC. | Jian Chao Bian Fu Min Shen Li Shen Tian Ru Wang Xiao Hong Wang Gong Chao Chen Jin Bing Wang Department of Epidemiology,Shanghai Medical University,Shanghai 200032,China Haimen Municipal Anti-Epidemic and Health Station,Haimen 226201,Jiangsu Province,China Qidong Institute for Liver Cancer,Qidong 226200,Jiangsu Province,China | 2000 | World Journal of Gastroenterology2000，6，2： | 12 |
| 7 | Identification of the epitopes on HCV core protein recognized by HLA-A2 restricted cytotoxic T lymphocytes显示文摘AIM To identify hepatitis C virus (HCV) core protein epitopes recognized by HLA-A2 restricted cytotoxic T lymphocyte (CTL).METHODS Utilizing the method of computer prediction followed by a 4 h 51 Cr-release assay confirmation.RESULTS The results showed that peripheral blood mononuclear cells (PBMC) obtained from two HLA-A2 positive donors who were infected with HCV could lyse autologous target cells labeled with peptide 'ALAHGVFAL (core TS0-158)'.The rates of specific lysis of the cells from the two donors were 37.5% and 15.8%,respectively. Blocking of the CTL response with anti-CD4 mAb caused no significant decrease of the specific lysis.But blocking of CTL response with anti-CD8 mAb could abolish the Iysis.CONCLUSION The peptide (core 150 - 158 ) is the candidate epitope recognized by HLA-A2 restricted CTL. | Hong-Chao Zhou De-Zhong Xu Xue-Ping Wang Jing-Xia Zhang Ying-Huang Yong-Ping Yan Yong Zhu Bo-Quan Jin Department of Epidemiology,the Fourth Military Medical University,Xi’an 710033,Shaanxi Province,ChinaDepartment of Immunology,the Fourth Military Medical University,Xi’an 710033,Shaanxi Province,China | 2001 | World Journal of Gastroenterology2001，7，4： | 11 |
| 8 | Is inconsistency of α-fetoprotein level a good prognosticator for hepatocellular carcinoma recurrence?显示文摘AIM: To identify the clinical outcomes of hepato-cellular carcinoma (HCC) patients with inconsistent α-fetoprotein (AFP) levels which were initially high and then low at recurrence.METHODS: We retrospectively included 178 patients who underwent liver resection with high preoperative AFP levels (≥ 200 ng/dL). Sixty-nine HCC patients had recurrence during follow-up and were grouped by their AFP levels at recurrence: group Ⅰ, AFP ≤ 20 ng/dL (n = 16); group Ⅱ, AFP 20-200 ng/dL (n = 24); and group Ⅲ, AFP ≥ 200 ng/dL (n = 29). Their preoperative clinical characteristics, accumulated recurrence rate, and recurrence-to-death survival rate were compared. Three patients, one in each group, underwentliver resection twice for primary and recurrent HCC. AFP immunohistochemistry of primary and recurrent HCC specimens were examined.RESULTS: In this study, 23% of patients demon-strated normal AFP levels at HCC recurrence. The AFP levels in these patients were initially high. There were no significant differences in clinical characteristics between the three groups except for the mean recur-rence interval (21.8 ± 14.6, 12.3 ± 7.7, 8.3 ± 6.6 mo, respectively, P < 0.001) and survival time (40.2 ± 19.9, 36.1 ± 22.4, 21.9 ± 22.0 mo, respectively, P = 0.013). Tumor size > 5 cm, total bilirubin > 1.2 mg/dL, vessel invasion, Child classification B, group Ⅲ, and recurrence interval < 12 mo, were risk factors for survival rate. Cox regression analysis was performed and vessel invasion, group Ⅲ, and recurrence interval were independent risk factors. The recurrence inter-val was significant longer in group Ⅰ (P < 0.001). The recurrence-to-death survival rate was significantly bet-ter in group Ⅱ (P = 0.016). AFP staining was strong in the primary HCC specimens and was reduced at recur-rence in group Ⅰ specimens.CONCLUSION: Patients in group Ⅰ with inconsistent AFP levels had a longer recurrence interval and worse recurrence-to-death survival rate than those in group Ⅱ. This clinical presentation may be caused by a delay in the detection of HCC recurrence. | Chung-Bao Hsieh, Teng-Wei Chen, Division of General Sur-gery, Tri-Service General Hospital, Taipei 114, Taiwan, China Chung-Bao Hsieh, Kuo-Piao Chung, Graduate Institute of Health Care Organization Administration, National Taiwan University, Taipei 100, Taiwan, China Chi-Ming Chu, Section of Biostatistics and Informatics, De-partment of Epidemiology, School of Public Health, National Defense Medical Center, Taipei 114, Taiwan, ChinaHeng-Cheng Chu, Department of Internal Medicine, Tri-Service General Hospital, Taipei 114, Taiwan, ChinaCheng-Ping Yu, Department of Pathology, Tri-Service General Hospital, Taipei 114, Taiwan, China | 2010 | World Journal of Gastroenterology2010，16，24： | 11 |
| 9 | Single-nucleotide polymorphisms among microRNA:big effects on cancer显示文摘MicroRNAs(miRNAs) are small non-coding RNAs that regulate gene expression at the transcriptional or posttranscriptional level.Many miRNAs are found to play a significant role in cancer development either as tumor suppressor genes or as oncogenes.Examination of tumor-specific miRNA expression profiles in diverse cancers has revealed widespread deregulation of these molecules,whose loss and overexpression respectively have diagnostic and prognostic significance.Genetic variations,mostly single-nucleotide polymorphisms(SNPs) within miRNA sequences or their target sites,have been found to be associated with many kinds of cancers.In this review,we summarize the current knowledge of miRNAs including their biogenesis and role in cancer development,and finally,how SNPs among miRNAs affect miRNA biogenesis and contribute to cancer. | Feng-Ju Song1,2 and Ke-Xin Chen1,2 Department of Epidemiology and Biostatistics,1Tianjin Medical University Cancer Hospital and Institute,Tianjin 300060,P.R.China Key Laboratory of Breast Cancer Prevention and Therapy,2Tianjin Medical University,Ministry of Education,Tianjin 300060,P.R.China | 2011 | Chinese Journal of Cancer2011，30，6： | 11 |
| 10 | Local Outbreak of COVID-19 in Shunyi District Attributed to an Asymptomatic Carrier with a History of Stay in Indonesia—Beijing Municipality,China,December 23,2020显示文摘Summary What is known about this topic?Patients with coronavirus disease 2019(COVID-19)infection can be categorized by severity:asymptomatic infection,mild illness,moderate illness,severe illness,and critical illness.The rate of transmission to a specific group of contacts(the secondary attack rate)may be 3-25 times lower from people who are asymptomatically infected than from those with symptoms.The incubation period is 2-14 days. | COVID-19 Epidemiology Investigation Team Laboratory Testing Team Wenzeng Zhang | 2021 | China CDC weekly2021，3，10： | 9 |
| 11 | Genomic research for important pathogenic bacteria in China显示文摘Rapid accumulation of bacterial genomic data offered an unprecedented opportunity to understand bacterial biology from a holistic view of point.We can thus closely look at the way in which a pathogen is evolved,and these data has been applied to molecular epidemiology and microbial forensics,and screening of novel diagnostic,vaccine and drug targets.The newly developed high-throughput low-cost sequencing technologies,such as 454,Solexa and SOLiD,will promote the acquisition and application of genomic data in new research areas that we dared not imagine previously,such as the metagenomics of human gastric-intestinal tract,for better and comprehensive understanding of human health and disease. | YANG RuiFu1,GUO XiaoKui2,YANG Jian3,JIANG YongQiang1,PANG Bo4,CHEN Chen5,YAO YuFeng2,QIN JinHong2 & LI QingTian2 1 Laboratory of Analytical Microbiology,State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology,Beijing 100071,China 2 Department of Medical Microbiology and Parasitology,Institutes of Medical Sciences,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China 3 State Key Laboratory for Molecular Virology and Genetic engineering,Institute of Pathogen Biology,Chinese Academy of Medical Sciences,Beijing 100176,China 4 State Key Laboratory for Infectious Disease Prevention and Control,Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China 5 Beijing Genomics Institute at Shenzhen,Shenzhen 518083,China | 2009 | Science China(Life Sciences)2009，52，1： | 8 |
| 12 | Construction and characterization of an HCV-derived multi-epitope peptide antigen containing B-cell HVR1 mimotopes and T-cell conserved epitopes显示文摘Hepatitis C(HCV) genome is highly variable,particularly in the hypervariable region 1 (HVR1) of its E2 envelope gene.The variability of HCV genome has been a major obstacle for de-veloping HCV vaccines.Due to B-cell HVR1 mimotopes mimicking the antigenicity of natural HVR1 epitopes and some T-cell epitopes from the consensus sequence of HCV genes conserving among the different HCV genotypes,we synthesized an minigene of HCV-derived multi-epitope peptide an-tigen(CMEP) ,which contains 9 B-cell HVR1 mimotopes in E2,2 conserved CTL epitopes in C,1 conserved CTL epitope in NS3 and 1 conserved Th epitope in NS3.This minigene was cloned into a GST expression vector to generate a fusion protein GST-CMEP.The immunogenic properties of CEMP were characterized by HCV infected patients' sera,and found that the reactivity frequency reached 75%.The cross reactivity of anti-CEMP antibody with different natural HVR1 variants was up to 90%.Meanwhile,we constructed an HCV DNA vaccine candidate,plasmid pVAX1.0-st-CMEP carrying the recombinant gene(st) of a secretion signal peptide and PADRE universal Th cell epitope sequence in front of the CMEP minigene.Immunization of rabbits with pVAX1.0-st-CMEP resulted in the production of antibody,which was of the same cross reactivity as the fusion protein GST-CMEP.Our findings indicate that the HCV-derived multi-epitope peptide antigen in some degree possessed the characteristics of neutralizing HCV epitopes,and would be of the value as a candidate for the development of HCV vaccines. | GAO Jun1,2,GONG Yuping1,ZHAO Ping1,ZHU Qing3,YANG Xiaoping1 & QI Zhongtian1 1.Department of Microbiology,Stake Key Laboratory of Medical Immunology,Second Military Medical University,Shanghai 200433,China 2.Department of Gastroenterology,Changhai Hospital,Second Military Medical University,Shanghai 200433,China 3.Department of Epidemiology,Naval Medical Research Institute,Shanghai 200433,China | 2006 | Science China(Life Sciences)2006，49，5： | 7 |
| 13 | 2005年广州市结核病现况抽样调查显示文摘目的了解广州市结核病的流行现状及危害程度,评价广州市结核病防治措施,为制定广州市2006至2010年结核病防治规划提供科学依据。方法采用整群随机和按人口比例抽样方法,抽样人口比例为1:158。广州市应有流行病学调查点为24个,平均每个调查点的应检人数为1656人。对调查点0~14岁儿童进行结核菌素纯蛋白衍生物试验,询问其卡介苗接种史并检查卡痕;对结核菌素反应≥10 mm,或虽<10 mm 但伴有水疱等强反应的儿童和≥15岁应检人口进行 X 线胸透;对疑有肺结核症状者、X 线胸透有异常阴影及已知肺结核患者均拍摄胸部 X 线片;对 X 线胸透异常及疑有肺结核症状者进行痰涂片和痰培养检查,对培养阳性的标本进行菌种鉴定。结果广州市活动性肺结核患病率为201/10万,涂阳肺结核患病率为33/10万,菌阳肺结核患病率为43/10万;14岁以下儿童中未发现活动性肺结核患者,15~50岁组活动性肺结核患病率为72.5/10万~176.8/10万,80岁以上达高峰,以老年男性最高(2341/10万);已知活动性肺结核、涂阳和菌阳肺结核分别为35.0%(28/80)、61.5%(8/13)和47.1%(8/17);初治活动性肺结核、涂阳和菌阳肺结核分别为81.3%(65/80)、69.2%(9/13)和58.8%(10/17);55岁以上组的复治活动性肺结核患者占80.0%。结论广州市的结核病患病率仍较高,老年菌阳肺结核仍较多。 | Guangzhou Technique Steering Group of the Epidemiological Sampling Survey for Tuberculosis | 2007 | 中华结核和呼吸杂志2007，30，6： | 6 |
| 14 | Meta analysis of risk factors for colorectal cancer显示文摘AIM: To study the risk factors for colorectal cancer in China.METHODS: A meta-analysis of the risk factors of colorectal cancer was conducted for 14 case-control studies, and reviewed 14 reports within 13 years which included 5034cases and 5205 controls. Dersimonian and Laird random effective models were used to process the results.RESULTS: Meta analysis of the 14 studies demonstrated that proper physical activites and dietary fibers were protective factors (pooled OR<0.8), while fecal mucohemorrhage,chronic diarrhea and polyposis were highly associated with colorectal cancer (all pooled OR>4). The stratified results showed that different OR values of some factors were due to geographic factors or different resourses.CONCLUSION: Risks of colorectal cancer are significantly associated with the histories of intestinal diseases or relative symptoms, high lipid diet, emotional trauma and family history of cancers. The suitable physical activities and dietary fibers are protective factors. | Kun Chen Jiong-Liang Qiu Yang Zhang Yu-Wan Zhao, Department of Epidemiology, School of Medicine, Zhejiang University, Hangzhou 310031, Zhejiang Province, China | 2003 | World Journal of Gastroenterology2003，9，7： | 6 |
| 15 | 流脑流行与人群抗体水平及带菌状况关系之探讨显示文摘通过80年代流脑流行周期的系统观察,首次阐明流脑流行与人群抗体水平及带菌状况之相关关系。观察结果显示,当流脑疫情处于散发期,流脑发病率及人群带菌率均处于低水平,人群流脑抗体水平也随之下降。当抗体水平降到低水平时。人群带菌率、流脑发病开始上升.然后抗体水平也随之升高.使用A群脑膜炎多糖菌苗免疫人群后,抗体水平显著上升,流脑发病、人群带菌明显下降。了解了这种变化规律,可早期观察流脑流行趋势,有利于采取各种防疫措施,指导流脑菌苗的接种工作,提高菌苗接种的经济效益。 | WuGuei kuen et al,(Collaboration Group of 7 Provincial Sanitary and Anti-epidemic Stations and Institute of Epidemiology and Microbiology(Beijing 100000) | 1994 | 中国公共卫生学报1994，13，4： | 6 |
| 16 | Establishment of a simple assay in vitro for hepatitis C virus NS3 serine protease based on recombinant substrate and single-chain protease显示文摘AIM: To establish a simple and convenient assay in vitro for the Hepatitis C virus NS3 serine protease based on the recombinant protease and substrate, and to evaluate its feasibility in screening the enzyme inhibitors. METHODS: Based on the crystallographic structure of hepatitis C virus (HCV) serine protease, a novel single-chain serine protease was designed, in which the central sequence of cofactor NS4A was linked to the N-terminus of NS3 serine protease domain via a flexible linker GSGS. The fusion gene was obtained by two-step PCR that was carried out with three primers and then cloned into the prokaryotic expression vector pQE30, and the recombinant clone was verified by DNA sequencing. The single-chain recombinant protease was expressed when the E.coliwas induced with IPTG and the expression conditions were optimized to produce large amount of soluble protease. The recombinant substrate NS5ab that covers the cleavage point NS5A/B was also expressed in E.coli. Both of the protease and substrate were purified by using Ni-NTA agarose metal affinity resin, then they were mixed together in a specific buffer, and the mixture was analyzed by SDS-PAGE. The cleavage system was used to evaluate some compounds for their inhibitory activity on serine protease.RESULTS: The single-chain recombinant protease was overexpressed as soluble protein when the E. coliwas induced at a low dosage of IPTG (0.2 mM) and cultured at a low temperature (15℃). The protease was purified by using Ni-NTA agarose metal affinity resin (the purity is over 95 %).The recombinant substrate NS5ab was expressed in an insoluble form and could refold successfully after purification and dialysis. A simple and convenient assay in vitro was established, in which the purified single-chain serine protease could cleave the recombinant substrate NS5ab into two fragments that were visualized by SDS-PAGE. PMSF had an effect on inhibiting activity of serine protease, while EDTA had not.CONCLUSION: A simple and convenient assayin vitro for hepatitis C virus NS3 serine protease is based on recombinant substrate NS5ab and single-chain serine protease. This assay can be used in screening of enzyme inhibitors. | Gui-Xin Du Li-Hua Hou Rong-Bin Guan Yi-Gang Tong Hai-Tao Wang,Department of Applied Molecular Biology,Institute of Microbiology and Epidemiology,Fengtai,Beijing 100071,China | 2002 | World Journal of Gastroenterology2002，8，6： | 6 |
| 17 | Evaluation of Impact of Major Causes of Death on Life Expectancy Changes in China,1990-2005显示文摘Objective To evaluate the impact of major causes of death on changes of life expectancy in China. Methods Life expectancy was calculated by standard life table techniques using mortality data from the national censuses in 1990 and 2000 and the 1% National Population Sampling Surveys in 1995 and 2005. Mortality data about the major causes of death from VR-MOH were used as reference values to estimate their death proportions of the specific age groups by sex and regions, as well as all-cause mortality and age-specific mortality rates of major causes of death. Decomposition method was used to quantitatively evaluate the impact. Results Three key findings were identified in our study. First, China's health challenge was shifted from diseases related to living conditions to those related to behavior and lifestyle, with rural areas relatively lagged behind urban areas. Second, the impacts of cardiovascular diseases and neoplasm on the middle aged and elderly population were stressed. Third, compared to the urban population, the rural population tended to have increasing mortality of neoplasm and cardiovascular diseases, especially in adults at the age of 15-39 years. Conclusion Further efforts should be made to reduce the incidence of neoplasm and cardiovascular diseases, especially in rural areas, by promoting healthy behavior and lifestyle and providing appropriate therapies for all patients in need. | YAN-HONG WANG AND LI-MING LI*1 *Department of Epidemiology & Biostatistics, School of Public Health, Peking University Health Science Center, Beijing 100191, China | 2009 | Biomedical and Environmental Sciences2009，22，5： | 6 |
| 18 | 浙江省1994~1995年糖尿病流行病学调查报告显示文摘为阐明浙江省成人糖尿病患病率及分布状况,由5个地市的部分医院组成了糖尿病调查协作组,按全国糖尿病流行病学调查方案,于1994年11月至1995年3月,抽样调查和统计分析了25岁以上常住人口7949人,糖尿病患病率为2.75%;从45~54岁组起患病率明显增高,且随增龄而呈上升趋势;超体重人群患病率是非超体重者的3.22倍;城市患病率显著高于农村和渔村;杭州、宁波、温州三地区间患病率无统计学上差异;和1974~1981年我省第一次糖尿病调查相比,患病率上升了5. | (The Zhejiang Province Diabetes Epidemiological Investigation Group) | 1998 | 科技通报1998，14，1： | 5 |
| 19 | Complex positive selection pressures drive the evolution of HIV-1 with different co-receptor tropisms显示文摘HIV-1 co-receptor tropism is central for understanding the transmission and pathogenesis of HIV-1 infection. We performed a genome-wide comparison between the adaptive evolution of R5 and X4 variants from HIV-1 subtypes B and C. The results showed that R5 and X4 variants experienced differential evolutionary patterns and different HIV-1 genes encountered various positive selection pressures, suggesting that complex selection pressures are driving HIV-1 evolution. Compared with other hypervariable regions of Gp120, significantly more positively selected sites were detected in the V3 region of subtype B X4 variants, V2 region of subtype B R5 variants, and V1 and V4 regions of subtype C X4 variants, indicating an association of positive selection with co-receptor recognition/binding. Intriguingly, a significantly higher proportion (33.3% and 55.6%, P<0.05) of positively selected sites were identified in the C3 region than other conserved regions of Gp120 in all the analyzed HIV-1 variants, indicating that the C3 region might be more important to HIV-1 adaptation than previously thought. Approximately half of the positively selected sites identified in the env gene were identical between R5 and X4 variants. There were three common positively selected sites (96, 113 and 281) identified in Gp41 of all X4 and R5 variants from subtypes B and C. These sites might not only suggest a functional importance in viral survival and adaptation, but also imply a potential cross-immunogenicity between HIV-1 R5 and X4 variants, which has important implications for AIDS vaccine development. | ZHANG ChiYu1*, DING Na1, CHEN KePing1 & YANG RongGe2* 1Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China 2HIV Molecular Epidemiology and Virology Research Group, State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China | 2010 | Science China(Life Sciences)2010，53，10： | 5 |
| 20 | A群脑膜炎多糖菌苗接种前后流脑流行规律的改变及今后预防对策显示文摘本文研究了在使用菌苗预防前后的流行性脑脊髓膜炎流行强度的变迁,年龄、地区及季节分布特点,人群的易感性及带菌率之间的关系,并讨论了今后的预防措施。 | Wu Gueikuens, et al., Collaboration of 7 Provincial Sanitary and Anti-epidemic Station and Institute of Epidemiology and Microbiology (Beijing 100000) | 1994 | 中国公共卫生学报1994，13，5： | 5 |
您所在的机构暂未开通此部分的功能，请联系管理员