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您的检索式：作者名="Elizabeth Robello"
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| 1 | Soybean Ferritin:Isolation,Characterization,and Free Radical Generation显示文摘The main aim of this work was to assess the multi-task role of ferritin(Ft)in the oxidative metabolism of soybean(Glycine max).Soybean seeds incubated for 24 h yielded 41 ± 5 μg Ft/g fresh weight.The rate of in vitro incorporation of iron(Fe)into Ft was tested by supplementing the reaction medium with physiological Fe chelators.The control rate,observed in the presence of 100 μM Fe,was not significantly different from the values observed in the presence of 100 μM Fe-his.However,it was significantly higher in the presence of 100 μM Fe-citrate(approximately 4.5-fold)or of 100 μM Fe-ATP(approximately 14-fold).Moreover,a substantial decrease in the Trp-dependent fluorescence of the Ft protein was determined during Fe uptake from Fe-citrate,as compared with the control.On the other hand,Ft addition to homogenates from soybean embryonic axes reduced endogenously generated ascorbyl radical,according to its capacity for Fe uptake.The data presented here suggest that Ft could be involved in the generation of free radicals,such as hydroxyl radical,by Fe-catalyzed reactions.Moreover,the scavenging of these radicals by Ft itself could then lead to protein damage.However,Ft could also prevent cellular damage by the uptake of catalytically active Fe. | Andrea Galatro Elizabeth Robello Susana Puntarulo | 2012 | Journal of Integrative Plant Biology2012，54，1： | 1 |
| 2 | Update on Fe-dependent oxidative metabolism in vivo:An integrative view显示文摘Fe is essential for human life because it constitutes the required cofactor for proteins of diverse biological functions.However,the development of oxidative stress by exposure to excessive Fe,share signaling pathways with other treatments including activation of redox-sensitive factors.This study was focused on the comparison on the effects of Fe in the brain and other organs in vivo.The oxidative effects triggered by Fe overload strongly depend not only on the administration protocol,but also on the Fe-compound used,and the studied organ.In both the liver and the brain,Fe content drastically increased after Fe-dextran administration.However,the comparatively low lipid peroxidation in the brain as compared to the liver,suggested that Fe-dependent oxidative stress might involve mechanisms of different nature.In the brain,acute and subchronic administration of Fe-dextran triggered signaling processes that lead to the prevention of injury by the participation of catalase activity as an antioxidant protection.This brief summary opens a huge range of possible points of risk,as well as opportunities,to encounter situations in which the appropriate election of the Fe management protocol could be able of allow oxidative stress to exert beneficial effects. | NATACHA E PILONI ELIZABETH ROBELLO JULIAN G BONETTO SUSANA PUNTARULO | 2016 | BIOCELL2016，40，1： | 0 |
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