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| 1 | Events associated with apoptotic effect of p-Coumaric acid in HCT-15 colon cancer cells显示文摘AIM:To investigate the events associated with the apoptotic effect of p-Coumaric acid,one of the phenolic components of honey,in human colorectal carcinoma(HCT-15)cells.METHODS:3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltertazolium-bromide assay was performed to determine the antiproliferative effect of p-Coumaric acid against colon cancer cells.Colony forming assay was conducted to quantify the colony inhibition in HCT15 and HT 29 colon cancer cells after p-Coumaric acid treatment.Propidium Iodide staining of the HCT15 cells using flow cytometry was done to study the changes in the cell cycle of treated cells.Identification of apoptosis was done using scanning electron microscope and photomicrograph evaluation of HCT 15cells after exposing to p-Coumaric acid.Levels of reactive oxygen species(ROS)of HCT 15 cells exposed to p-Coumaric acid was evaluated using 2’,7’-dichlorfluorescein-diacetate.Mitochondrial membrane potential of HCT-15 was assessed using rhodamine-123 with the help of flow cytometry.Lipid layer breaks associated with p-Coumaric acid treatment was quantified using the dye merocyanine 540.Apoptosis was confirmed and quantified using flow cytometric analysis of HCT15 cells subjected to p-Coumaric acid treatment after staining with YO-PRO-1.RESULTS:Antiproliferative test showed p-Coumaric acid has an inhibitory effect on HCT 15 and HT 29 cells with an IC50(concentration for 50%inhibition)value of 1400 and 1600μmol/L respectively.Colony forming assay revealed the time-dependent inhibition of HCT 15 and HT 29 cells subjected to p-Coumaric acid treatment.Propidium iodide staining of treated HCT 15cells showed increasing accumulation of apoptotic cells(37.45±1.98 vs 1.07±1.01)at sub-G1phase of the cell cycle after p-Coumaric acid treatment.HCT-15 cells observed with photomicrograph and scanning electron microscope showed the signs of apoptosis like blebbing and shrinkage after p-Coumaric acid exposure.Evaluation of the lipid layer showed increasing lipid layer breaks was associated with the growth inhibition of p-Coumaric acid.A fall in mitochondrial membrane potential and increasing ROS generation was observed in the p-Coumaric acid treated cells.Further apoptosis evaluated by YO-PRO-1 staining also showed the timedependent increase of apoptotic cells after treatment.CONCLUSION:These results depicted that p-Coumaric acid inhibited the growth of colon cancer cells by inducing apoptosis through ROS-mitochondrial pathway. | Saravana Kumar Jaganathan Eko Supriyanto Mahitosh Mandal | 2013 | World Journal of Gastroenterology2013，19，43： | 12 |
| 2 | Role of pomegranate and citrus fruit juices in colon cancer prevention显示文摘Colorectal cancer is the second leading cause of cancer-related deaths in the United States.Recent studies prove that though chemotherapeutic agents are being used for the treatment of colon cancer,they become non-effective when the cancer progresses to an invasive stage.Since consumption of certain dietary agents has been linked with various cancers,fruit juices have been investigated for their consistently protective effect against colon cancer.The unique biochemical composition of fruit juices is responsible for their anticancer properties.In this review,the chemo-preventive effect of fruit juices such as pomegranate and citrus juices against colon cancer are discussed.For this purpose,the bioavailability,in vitro and in vivo effects of these fruit juices on colorectal cancer are highlighted.Moreover,there is a scarcity of studies involving human trials to estimate the preventive nature of these juices against colon cancer.This review will support the need for more preclinical tests with these crude juices and their constituents in different colorectal cancer cell lines and also some epidemiological studies in order to have a better understanding and promote pomegranate and citrus juices as crusaders against colon cancer. | Saravana Kumar Jaganathan Muthu Vignesh Vellayappan Gayathri Narasimhan Eko Supriyanto | 2014 | World Journal of Gastroenterology2014，20，16： | 6 |
| 3 | Chemopreventive effect of apple and berry fruits against colon cancer显示文摘Colon cancer arises due to the conversion of precancerous polyps(benign)found in the inner lining of the colon.Prevention is better than cure,and this is very true with respect to colon cancer.Various epidemiologic studies have linked colorectal cancer with food intake.Apple and berry juices are widely consumed among various ethnicities because of their nutritious values.In this review article,chemopreventive effects of these fruit juices against colon cancer are discussed.Studies dealing with bioavailability,in vitro and in vivo effects of apple and berry juices are emphasized in this article.A thorough literature survey indicated that various phenolic phytochemicals present in these fruit juices have the innate potential to inhibit colon cancer cell lines.This review proposes the need for more preclinical evidence for the effects of fruit juices against different colon cancer cells,and also strives to facilitate clinical studies using these juices in humans in large trials.The conclusion of the review is that these apple and berry juices will be possible candidates in the campaign against colon cancer. | Saravana Kumar Jaganathan Muthu Vignesh Vellayappan Gayathri Narasimhan Eko Supriyanto Dyah Ekashanti Octorina Dewi Aqilah Leela T Narayanan Arunpandian Balaji Aruna Priyadarshini Subramanian Mustafa Yusof | 2014 | World Journal of Gastroenterology2014，20，45： | 3 |
| 4 | MiR-22 as a metabolic silencer and liver tumor suppressor显示文摘With obesity rate consistently increasing,a strong relationship between obesity and fatty liver disease has been discovered.More than 90%of bariatric surgery patients also have non-alcoholic fatty liver diseases(NAFLDs).NAFLD and non-alcoholic steatohepatitis(NASH),which are the hepatic manifesta-tions of metabolic syndrome,can lead to liver carcinogenesis.Unfortunately,there is no effective medicine that can be used to treat NASH or liver cancer.Thus,it is critically important to understand the mechanism underlying the development of these diseases.Extensive evidence suggests that microRNA 22(miR-22)can be a diagnostic marker for liver diseases as well as a treatment target.This review paper focuses on the roles of miR-22 in metabolism,steatosis,and liver carcinogenesis.Literature search is limited based on the publications included in the PubMed database in the recent 10 years. | Lijun Wang Yu-Shiuan Wang Eko Mugiyanto Wei-Chiao Chang Yu-Jui Yvonne Wan | 2020 | Liver Research2020，4，2： | 3 |
| 5 | Comparison of the morphology and structure of W03 nanomaterials synthesized by a sol-gel method followed by calcination^or hydrothermal treatment显示文摘 | Diah Susanti Stefanus Haryo N Hasnan Nisfu Eko Prasetio Nugroho Hariyati Purwaningsih GeorgeEndri Kusuma Shao-Ju Shih | 2012 | Frontiers of Chemical Science and Engineering2012，6，4： | 3 |
| 6 | Chemokine and chemokine receptor dynamics during genital chlamydial infection显示文摘 | Belay T Eko FO Ananaba GA | 2002 | Infect Immun2002，70，2： | 1 |
| 7 | Recombinant Vibrio cholerae ghosts as a delivery vehicle for vaccinating against Chlamydia trachomatis 显示文摘 | Eko FO Lubitz W McMillan L | 2003 | Vaccine2003，21，15： | 1 |
| 8 | Liquid-phase reaction of 2'-hydroxyacetophenone and benzaldehyde over SO3H-SBA-15 catalysts : Influence of microwave and thermal effects显示文摘 | Shunmugavel S Su J D Eko A P | 2008 | Microporous and Mesoporous Materials2008，112，： | 1 |
| 9 | The concentration of circulating corticotropin-releasing hormone mRNA in maternal plasma is increased in preeclampsia显示文摘 | Ng EKO Leung TN Tsui NBY | 2003 | Clin Chem2003，49，5： | 1 |
| 10 | Bacterial ghosts:non-living candidate vaccines显示文摘 | Szostak MP Hensel A Eko FO | 1996 | J Biotechnol1996，44，13： | 1 |
| 11 | Immunogenicity of a novel recombinant subunit candidate vaccine against Chlamydia trachomatis显示文摘 | Eko F O Lubitz W Igietseme J U | 2001 | Proceedings of the 101st General Meeting of the American Society for Microbiology2001，341，： | 1 |
| 12 | Developing effective delivery systems for Chlamydia vaccines显示文摘 | Igietseme JU Eko FO He Q | 2004 | Curr Opin Mol Ther2004，6，： | 1 |
| 13 | Bacterialg- hosts: non-livincandidat evaccines 显示文摘 | Szostak M P Hensel A Eko F O | 1996 | J Biotechnol1996，44，： | 1 |
| 14 | Bacterial ghosts: nonliving candidate vaccines显示文摘 | SZOSTAK M P HENSEL A EKO F O | 1996 | J Biotechnol1996，44，3： | 1 |
| 15 | mRNA of placental origin is readily detectable in maternal plasma显示文摘 | Ng EKO Tsui NBY Lau TK | | 0，，： | 1 |
| 16 | Characterization of the Gold Ore to Acquire an Optimum Degree of Liberation显示文摘 | Waterman Sulistyana Bargawa Eko Hardiyanto | 2017 | Journal of Environmental Science and Engineering(B)2017，6，6： | 1 |
| 17 | Induction of immune memory by a muhisubunit chlamydial vaccine 显示文摘 | Eko FO Ekong E He Q | 2011 | Vaccine2011，29，7： | 1 |
| 18 | Extended recombi- nant bacterial ghost system显示文摘 | Lubitz W Witte A Eko F O | 1999 | J Biotechnol1999，73，： | 1 |
| 19 | Bacterial ghosts as carrier and targeting systems for mucosal antigen delivery 显示文摘 | JALAVA K EKO F O RIEDMANN E | 2003 | Expert Rev Vaccines2003，2，1： | 1 |
| 20 | mRNA of placental origin is readily detectable in ma- ternal plasma显示文摘 | Ng EKO | 2003 | Proc Natl Acad Sci USA2003，100，： | 1 |
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