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| 1 | Probiotic metabolites from Bacillus coagulans GanedenBC30^(TM) support maturation of antigen-presenting cells in vitro显示文摘AIM:To study the effects of probiotic metabolites on maturation stage of antigen-presenting immune cells.METHODS:Ganeden Bacillus coagulans 30(GBC30) bacterial cultures in log phase were used to isolate the secreted metabolite(MET) fraction.A second fraction was made to generate a crude cell-wall-enriched fraction,by centrifugation and lysis,followed by washing.A preparation of MET was subjected to size exclusion centrifugation,generating three fractions:< 3 kDa,3-30 kDa,and 30-200 kDa and activities were tested in comparison to crude MET and cell wall in primary cultures of human peripheral blood mononuclear cell(PBMC) as a source of antigen-presenting mononuclear phagocytes.The maturation status of mononuclear phagocytes was evaluated by staining with monoclonal antibodies towards CD14,CD16,CD80 and CD86 and analyzed by flow cytometry.RESULTS:Treatment of PBMC with MET supported maturation of mononuclear phagocytes toward both macrophage and dendritic cell phenotypes.The biological activity unique to the metabolites included a reduction of CD14+ CD16+ pro-inflammatory cells,and this property was associated with the high molecular weight metabolite fraction.Changes were also seen for the dendritic cell maturation markers CD80 and CD86.On CD14dim cells,an increase in both CD80 and CD86 expression was seen,in contrast to a selective increase in CD86 expression on CD14bright cells.The co-expression of CD80 and CD86 indicates effective antigen presentation to T cells and support of T helper cell differentiation.The selective expression of CD86 in the absence of CD80 points to a role in generating T regulatory cells.CONCLUSION:The data show that a primary mechanism of action of GBC30 metabolites involves support of more mature phenotypes of antigen-presenting cells,important for immunological decision-making. | Kathleen F Benson Kimberlee A Redman Steve G Carter David Keller Sean Farmer John R Endres Gitte S Jensen | 2012 | World Journal of Gastroenterology2012，18，16： | 8 |
| 2 | Afro-assisted investigation of the corrosion behaviour of magnesium and Az91 alloys in an ionic liquid with varying water content显示文摘 | Shkurankov A E1 Abedin S Z Endres F | 2007 | Australian Journal of Chemistry2007，60，1： | 1 |
| 3 | 3-D mapping with an RGB-D camera显示文摘 | ENDRES F HESS J STURM J | 2014 | IEEE Transactions on Robotics2014，30，1： | 1 |
| 4 | Electrodeposition of nanocrystalline metals and alloys from ionic liquids显示文摘 | Endres F Bukowski M Hempelmann R et at | 2003 | Angewandte Chemie International Edition2003，42，29： | 1 |
| 5 | A study on the electrodeposition of tantalum on NiTi alloy in an ionic liquid and corrosion behaviour of the coated alloy显示文摘 | Zein El Abedin S Welz-Biermann U Endres F | 2005 | Electrochemistry Communications2005，7，9： | 1 |
| 6 | Air and Water Stable Ionic Liquids in Physical Chemistry显示文摘 | ENDRES F | 2006 | Physical Chemistry Chemical Physics2006，8，18： | 1 |
| 7 | High resolution sediment and vegetation responses to Younger Dryas climate change in varved lake sediments from Meerfelder Maar,Germany显示文摘 | Brauer A Endres C G nter C Litt T Stebich M Negendank J F W | | 0，，： | 1 |
| 8 | Ionic liquids:solvents for the electrodcposition of metals and semiconductors显示文摘 | Endres F | 2002 | Chem Phys Chem2002，3，2： | 1 |
| 9 | Direct low temperature synthesis of rufle nanostructures in ionic liquids显示文摘 | Kaper H Endres F Dierdj I et a1 | 2003 | Small2003，，10： | 1 |
| 10 | Vibrational spectroscopy of triazoles and tetrazole显示文摘 | Billes F Endre'dia H Kereszturyb G | 2000 | J Mol Struct (Theochem)2000，530，： | 1 |
| 11 | Electroplating of Mild Steel by Aluminium in a First Generation Ionic Liquid:A Green Alternative to Commercial Al-plating in Organic Solvents显示文摘 | Liu Q X El Abedin S Z Endres F | 2006 | Surface and Coatings Technology2006，201，34： | 1 |
| 12 | Electrodeposition of nanocrystalline metals and alloys from ionic liquids显示文摘 | Endres F Bukowski M Hempelmann R | 2003 | Angewandte Chemie International Edtion2003，42，29： | 1 |
| 13 | Ionic-liquidmaterials for the electrochemical challenges of the future显示文摘 | ARMAND M ENDRES F MACFARLANE D R | 2009 | NatureMater2009，8，： | 1 |
| 14 | Electroplating of mild steel by aluminum in a first generation ionic liquid:A green alternative to commercial Al-plating in organic solvents显示文摘 | Liu Q X EI Abedin S Z Endres F | 2006 | Surface and Coatings Technology2006，201，3： | 1 |
| 15 | Electrodeposition of nano- and microcrystalline aluminium in three different air and water stable ionic liquids显示文摘 | ZEIN E1 ABED1N S MOUSTAFA E M HEMPELMANN R NATTER H ENDRES F | 2006 | Chem Phys Chem2006，7，7： | 1 |
| 16 | Additive free electrodeposition of nanocrystalline aluminium in a water and air stable ionic liquid显示文摘 | ZEIN EI ABEDIN S MOUSTAFA E M HEMPELMANN R NATTER H ENDRES F | 2005 | Electrochemistry Communications2005，7，11： | 1 |
| 17 | Ionic-liquid materials for the electrochemical challenges of the future 显示文摘 | Armand M Endres F MacFarlane D F | 2009 | Nat Mater2009，8，： | 1 |
| 18 | AFM-assisted investigation of the corrosion behavior of magnesium and AZ91 alloys in an ionic liquid with varying water content显示文摘 | SHURANKOY A EL ABEDIN S Z ENDRES F | 2007 | Aust J Chem2007，60，1： | 1 |
| 19 | Direct low-temperature synthesis of rutile nanostructures in ionic liquids显示文摘 | Kaper H Endres F Djerdj I | 2007 | Small2007，3，10： | 1 |
| 20 | An evaluation of the RGB-D SLAM system显示文摘 | Endres F Hess J Engelhard N | 2012 | IEEE International Conference on Ro- botics and Automation2012，162，4： | 1 |
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