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| 1 | Low-cost,simple,and scalable self-assembly of DNA origami nanostructures显示文摘Despite demonstrating exciting potential for applications such as drug delivery and biosensing,the development of nanodevices for practical applications and broader use in research and education are still hindered by the time,effort,and cost associated with DNA origami fabrication.Simple and robust methods to perform and scale the DNA origami self-assembly process are critical to facilitate broader use and translation to industrial or clinical applications.We report a simple approach to fold DNA origami nanostructures that is fast,robust,and scalable.We demonstrate fabrication at scales approximately 100-1,500-fold higher than typical scales.We further demonstrate an approach we termed low-cost efficient annealing (LEAN) self-assembly involving initial heating at 65 ℃ for 10 min,then annealing at 51 ℃ for 2 h,followed by brief quenching at 4 ℃ that leads to effective assembly of a range of DNA origami structures tested.In contrast to other methods for scaling DNA origami assembly,this approach can be carried out using cheap and widely available equipment (e.g.,hot plates,water baths,and laboratory burners) and uses standard recipes and materials so is readily applied to any existing or new DNA origami designs.We envision these methods can facilitate device development for commercial applications and facilitate broader use of DNA origami in research and education. | Patrick D.Halley Randy A.Patton Amjad Chowdhury John C.Byrd Carlos E.Castro | 2019 | Nano Research2019，12，5： | 3 |
| 2 | MicroRNA‐143 reduces viability and increases sensitivity to 5‐fluorouracil in HCT116 human colorectal cancer cells显示文摘 | Pedro M.Borralho Betsy T.Kren Rui E.Castro Isabel B.Moreira da Silva Clifford J.Steer Cecília M. P.Rodrigues | 2009 | FEBS Journal2009，，22： | 1 |
| 3 | Evaluating research efficiency within National R&D Programmes显示文摘 | J.Femando J.Mikel L.Zofioc E.Castro | | 0，，40： | 1 |
| 4 | Interleukin (IL)‐22, IL‐17, IL‐23, IL‐8, vascular endothelial growth factor and tumour necrosis factor‐α levels in patients with psoriasis before, during and after psoralen–ultraviolet A and narrowband ultraviolet B therapy显示文摘 | S.Coimbra H.Oliveira F.Reis L.Belo S.Rocha A.Quintanilha A.Figueiredo F.Teixeira E.Castro P.Rocha‐Pereira A.Santos‐Silva | 2010 | British Journal of Dermatology2010，，6： | 1 |
| 5 | Methods used in microbial forensics and epidemiological investigations for stronger health systems显示文摘This review discusses microbial forensics as an emerging science that finds application in protecting human health.It is important to distinguish naturally acquired infections from those caused by the intentional release of microorganisms to the environment.This information is crucial in formulating procedures against the spread of infectious diseases and prosecuting persons who may be involved in acts of biocrime,bioterrorism,or biowarfare.A comparison between epidemiological investigations and microbial forensic investigations is provided.In addition,a discussion on how microbial forensics strengthens health systems is included in this review.Microbial forensic investigations and epidemiologic examinations employ similar concepts and involve identifying and characterising the microbe of interest.Both fields require formulating an appropriate case definition,determining a pathogen’s mode of transmission,and identifying the source(s)of infection.However,the two subdisciplines differ in their objectives.An epidemiological investigation aims to identify the pathogen’s source to prevent the spread of the disease.Microbial forensics focuses on source-tracking to facilitate the prosecution of persons responsible for the spread of a pathogen.Both fields use molecular techniques in analysing and comparing DNA,gene products,and biomolecules to identify and characterise the microorganisms of interest.We included case studies to show methods used in microbial forensic investigations,a brief discussion of the public significance of microbial forensic systems,and a roadmap for establishing a system at a national level.This system is expected to strengthen a country’s capacity to respond to public health emergencies.Several factors must be considered in establishing national microbial forensic systems.First is the inherent ubiquity,diversity,and adaptability of microorganisms that warrants the use of robust and accurate molecular typing systems.Second,the availability of facilities and scientists who have been trained in epidemiology,molecular biology,bioinformatics,and data analytics.Human resources and infrastructure are critical requirements because formulating strategies and allocating resources in times of infectious disease outbreaks must be data-driven.Establishing and maintaining a national microbial forensic system to strengthen capacities in conducting forensic and epidemiological investigations should be prioritised by all countries,accompanied by a national policy that sets the legislative framework and provides for the system’s financial requirements. | Arizaldo E.Castro Maria Corazon A.De Ungria | 2022 | Forensic Sciences Research2022，7，4： | 0 |
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