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| 1 | Pm21 from Haynaldia villosa Encodes a CC-NBS- LRR Protein Conferring Powdery Mildew Resistance in Wheat显示文摘 | Liping Xing Ping Hu Jiaqian Liu Kamil Witek Shuang Zhou Jiefei Xu Weihao Zhou Li Gao Zhenpu Huang Ruiqi Zhang Xiue Wang Peidu Chen Haiyan Wang Jonathan D.G. Jones Miroslava Karafiatova Jan Vrana Jan Baros Jaroslav Dolezel Yuanchun Tian Yufeng Wu Aizhong Cao | 2018 | Molecular Plant2018，11，6： | 16 |
| 2 | Natural orifice transluminal endoscopic surgeryvs laparoscopic ovariectomy:Complications and inflammatory response显示文摘AIM:To compare natural orifice transluminal endoscopic surgery (NOTES) vs standard laparoscopic ovariectomy in mini pigs with respect to technical aspects,complications and parameters of systemic inflammatory response.METHODS:This was a randomized,experimental,survival study.Ten female mini pigs underwent NOTES transgastric ovariectomy (NOTES group) and ten female mini pigs underwent laparoscopic ovariectomy (LAP group).A 'percutaneous endoscopic gastrostomy' approach with guidewire and sphincterotome was used for gastrotomy creation.The ovary was resected using standard biopsy forceps and a snare.The access site was closed using a 'KING' closure with a single endoloop and several clips.In the laparoscopic group,a three-port laparoscopy and an ovariectomy were performed with the use of standard laparoscopic devices.C-reactive protein (CRP),white blood count and interleukin (IL)-6 plasma levels were used as indicators of systemic inflammatory response.All animals were euthanized 28 d after surgery.RESULTS:All animals survived without complications.The mean procedure time was 41.3 min ± 17.6 min (NOTES group) and 25.7 min ± 5.25 min (LAP group,P < 0.02).Postmortem examinations demonstrated that 50% and 70% of animals were free of any complications in the NOTES and LAP groups,respectively.The remaining animals developed minor complications (adhesions) in a comparable frequency between the two groups.In the NOTES group,one animal developed a small intramural gastric abscess close to the gastrotomy site.A minor serous exudate that was present in 50% and 40% of the animals in the NOTES and laparoscopy groups,respectively,was not considered a complication.In both groups CRP levels increased significantly on the 2nd and 7th postoperative days (POD) and returned to normal after 28 d.On POD 2,an increase of CRP level was significantly higher in the NOTES group compared to the LAP group.Values of IL-6 did not differ from baseline values in either of the groups postoperatively.Interestingly,the platelet count decreased significantly on POD 2,but returned close to baseline values on POD 7 and PODs 28-30.CONCLUSION:Both NOTES and laparoscopic ovariectomies had a similar frequency of minor complications.However,the NOTES technique produced an increased systemic inflammatory response on POD 2. | Jan Martínek Ondej Ryska Tereza Filípková Radek Dolezel Stefan Juhas Jan Motlík Monika Holubová Vladimír Nosek Barbora Rotnáglová Miroslav Zavoral Miroslav Ryska | 2012 | World Journal of Gastroenterology2012，18，27： | 4 |
| 3 | Development of oligonucleotide probes for FISH karyotyping in Haynaldia villosa,a wild relative of common wheat显示文摘Haynaldia villosa is a wild relative of wheat and a valuable gene resource for wheat improvement.Owing to the limited number of probes available for fluorescence in situ hybridization(FISH),the resolution at which the karyotype of H.villosa can be characterized is poor,hampering accurate characterization of small segmental alien introgressions.We designed ten oligonucleotide probes using tandem repeats in DNA sequences derived from the short arm of H.villosa chromosome 6 V(6 VS).FISH with seven of them resulted in clear signals on H.villosa chromosomes.Using these,we constructed FISH karyotypes for H.villosa using oligo-6 VS-1 and oligo-6 VS-35 oligonucleotides and characterized the distribution of the two probes in five different H.villosa accessions.The new FISH probes can efficiently characterize H.villosa introgressions into wheat. | Jia Lei Jiawen Zhou Haojie Sun Wentao Wan Jin Xiao Chunxia Yuan Miroslava Karafiátová Jaroslav Dolezel Haiyan Wang Xiue Wang | 2020 | The Crop Journal2020，8，4： | 3 |
| 4 | 基于纤毛鹅观草特异的卫星重复序列开发寡核苷酸探针显示文摘[目的]本文旨在开发纤毛鹅观草特异的寡核苷酸(oligonucleotide,oligo)探针,进一步完善纤毛鹅观草染色体鉴定技术。[方法]利用前期选育的普通小麦-纤毛鹅观草异附加系DA2S^(c)L和DA6S^(c),通过流式分拣和基因组二代测序获得纤毛鹅观草染色体6S^(c)和染色体臂2S^(c)长臂的基因组序列,利用Repeatexplorer2/TAREAN软件从中鉴定出卫星重复序列并设计成oligo探针,通过荧光原位杂交(fluorescence in situ hybridization,FISH)分析所开发oligo探针的应用价值。[结果]从6S^(c)和2S^(c)L基因组序列中共鉴定出11个卫星重复序列并开发成11个oligo探针,oligo-FISH结果表明,其中6个探针可以在纤毛鹅观草染色体上产生明显杂交信号,而在小麦染色体上未产生明显杂交信号,可用于特异鉴定小麦背景中的纤毛鹅观草染色体或片段。对1套普通小麦-纤毛鹅观草异附加系oligo-FISH分析发现,oligo-2S^(c)L^(-1)63和oligo-6S^(c)-111仅在S^(c)基因组染色体上产生明显信号,可作为纤毛鹅观草S^(c)组特异探针;将oligo-2S^(c)L-161和oligo-2S^(c)L-163组合,对1整套二体异附加系进行双色oligo-FISH,构建了纤毛鹅观草染色体的oligo-FISH核型。[结论]本研究提供了纤毛鹅观草重复序列组成的初步信息,开发的探针能特异鉴定纤毛鹅观草染色体,阐明纤毛鹅观草2个基因组的来源和分化,对推动纤毛鹅观草优异基因的转移和利用有重要意义。 | 程梦豪 Miroslava Karafiatova 孙昊杰 Kate Arina Holušva Jaroslav Dolezel 宋新颖 王海燕 王秀娥 | 2022 | 南京农业大学学报2022，45，3： | 2 |
| 5 | Analysis of nuclear DNA content in plant cells by flow cytometry显示文摘 | Dolezel J Binarova P Lucrettr S | 1989 | Biol Plant1989，31，： | 1 |
| 6 | Effect of plant tissue culture media on the frequency of somatic mutations in Tradescantia stamen hairs 显示文摘 | Novak F J | 1984 | Z Plfanzenphysiol1984，114，： | 1 |
| 7 | The influence of 2,4-dichlorophenoxyacetic acid on cell cycle kinetics and sister-chromatid exchange frequency in garlic (Alium sativum) meristem cells 显示文摘 | Lucretti S Novak F J | 1987 | Biologia Plantarum (Prague)1987，29，： | 1 |
| 8 | Sex determ ination in dioecious plants Melandrium album using high resolution flow cytometry显示文摘 | Dolezel J Gohde W | 1995 | Cytometry1995，19，： | 1 |
| 9 | Primer-induced labelling of pea and field bean chromosomes in situ and in suspension显示文摘 | Macas J Dolezel J Gualberti G | 1995 | Bio Techniques1995，19，： | 1 |
| 10 | Interfraction variation in prostate cancer-analysis of 11726cone-beam CT显示文摘 | Dolezel M Slezak P Odrazka K | 2015 | J BUON2015，20，4： | 1 |
| 11 | Nuclear DNA content and genome size of trout and human显示文摘 | Dolezel J Bartos J Voglmayr H | 2003 | Cytometry2003，51，2： | 1 |
| 12 | 采用感应预热的连续激光淬火显示文摘模拟了钢件采用激光束进行表面连续淬火的新方法。这种热处理方法采用传统的感应器对淬火零件进行预热,以便减小温度梯度及随后产生的被处理材料的机械应力。该工艺的数学模型采用3D数值建立,其解法考虑了所有重要的非线性特性(被淬火钢的磁饱和曲线及其物理性能与温度的关系)。用典型的实例进行了说明,介绍了结果并进行了讨论。 | I Dolezel V.Kotlan R.Hamar D.Pánek 张家雄 | 2015 | 热处理2015，30，2： | 1 |
| 13 | Is period gene causally involved in the photoperiodie regulation of reproductive diapause in the linden bug Pyrrhocoris apyerus显示文摘 | Dolezel D Vaneekova H Sauman I | 2005 | Insect Physio12005，51，： | 1 |
| 14 | Possible worlds of fiction and history 显示文摘 | Dolezel Lubomir | 1998 | New Literary History1998，29，4： | 1 |
| 15 | Comparison of three DNAfluorochromes for flow cytometric estimation of nuclear DNA contentin plants显示文摘 | DOLEZEL J SGORBATI S LUCRETTI S | 1992 | Physiologia Plantarum1992，85，4： | 1 |
| 16 | Nuclear DNA content and genome size of trout and human显示文摘 | Dolezel J Bartos J Voglmayr H | 2003 | Cytometry2003，51，： | 1 |
| 17 | Analysis of nuclear DNA content in plant cells by flow cytometry显示文摘 | Dolezel J Binrova P Lucretri S | 1989 | Biol Plantarum1989，31，2： | 1 |
| 18 | PlantDNA Flow Cytometry and Estimation of Nuclear Genome Size显示文摘 | BARTOS J | 2005 | Annals of Botany2005，95，1： | 1 |
| 19 | Induction and verification of autotetrap|oids in diploid banana(Musa acuminita)by in vitro techniques显示文摘 | Van Dunren M Morpurgo R Dolezel J | 1996 | Euphytcia1996，88，1： | 1 |
| 20 | Nuclear DNA content and in vitro induced somatic polyploidization cassava (Manihot esculenta Crantz) breeding显示文摘 | F. Awoleye M. Duren J. Dolezel F. J. Novak | 1994 | Euphytica1994，，3： | 1 |
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