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1篇 您的检索式:作者名="Divya Mistry"
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1Flor Revisited(Again):eQTL and Mutational Analysis of NB-LRR Mediated Immunity to Powdery Mildew in Barley显示文摘Genes encoding early signaling events in pathogen defense often are identif ied only by their phenotype.Such genes involved in barley-powdery mildew interactions include Mla,specifying race-specif ic resistance;Rar1(Required for Mla12-specif ied resistance1),and Rom1(Restoration of Mla-specif ied resistance1).The HSP90-SGT1-RAR1 complex appears to function as chaperone in MLA-specified resistance,however,much remains to be discovered regarding the precise signaling underlying plant immunity.Genetic analyses of fast-neutron mutants derived from CI 16151(Mla6)uncovered a novel locus,designated Rar3(Required for Mla6-specified resistance3).Rar3 segregates independent of Mla6 and Rar1,and rar3 mutants are susceptible to Blumeria graminis f.sp.hordei(Bgh)isolate 5874(AVR a6),whereas,wild-type progenitor plants are resistant.Comparative expression analyses of the rar3 mutant vs.its wild-type progenitor were conducted via Barley1 GeneChip and GAIIx paired-end RNA-Seq.Whereas Rar1 affects transcription of relatively few genes;Rar3 appears to inf luence thousands,notably in genes controlling ATP binding,catalytic activity,transcription,and phosphorylation;possibly membrane bound or in the nucleus.eQTL analysis of a segregating doubled haploid population identified over two-thousand genes as being regulated by Mla(q value/FDR=0.00001),a subset of which are signif icant in Rar3 interactions.The intersection of datasets derived from mla-loss-of-function mutants,Mla-associated eQTL,and rar3-mediated transcriptome reprogramming are narrowing the focus on essential genes required for Mla-specified immunity.Roger Wise Priyanka Surana Greg Fuerst Ruo Xu Divya Mistry Julie Dickerson Dan Nettleton 2014Journal of Integrative Agriculture2014,13,2:0
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