2篇
您的检索式：作者名="Ding Yanlu"
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Signal characteristics of coal and rock dynamics with micro-seismic monitoring technique显示文摘In this study,differences of signal characteristics between mine shocks and coal and gas outbursts in coal mines were examined with the micro-seismic monitoring technique and time–frequency analysis.The duration of the mine shock is short while the coal and gas outburst lasts longer.The outburst consists of three stages:the pre-shock,secondary shock and main shock stage,respectively.The velocity amplitude of the mine shock is between 10^(-)5and 10^(-3)m/s,which is higher than that of the outburst with the same energy level.In addition,in both cases,the correlation between the velocity amplitude and energy is positive while the correlation between the signal frequency band distribution and energy is negative.The signal frequency band of the high energy mine shock is distributed between 0 and 50 Hz,and the low energy mine shock is between 50 and 100 Hz.The fractal characteristics of mine shocks were studied based on a fractal theory.The box dimensions of high energy mine shocks are lower than the low energy ones,however,the box dimensions of outbursts are higher than that of mine shocks with the same energy level.The higher box dimensions indicate more dangerous dynamic events. | Ding Yanlu Dou Linming Cai Wu Chen Jianjun Kong Yong Su Zhenguo Li Zhenlei | 2016 | International Journal of Mining Science and Technology2016，26，4： | 2 |
| 2 | A preparation strategy for protein-oriented immobilized silica magnetic beads with Spy chemistry for ligand fishing显示文摘Due to the complexity of bioactive ingredients in biological samples,the screening of target proteins is a complex process.Herein,a feasible strategy for directing protein immobilization on silica magnetic beads for ligand fishing based on SpyTag/SpyCatcher(ST/SC)-mediated anchoring is presented.Carboxyl functional groups on the surface of silica-coated magnetic beads(SMBs)were coupled with SC using the 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride/N-hydroxysulfosuccinimide method,named SC-SMBs.The green fluorescent protein(GFP),as the capturing protein model,was ST-labeled and anchored at a specific orientation onto the surface of SC-SMBs directly from relevant cell lysates via ST/SC self-ligation.The characteristics of the SC-SMBs were studied via electron microscopy,energy dispersive spectroscopy,and Fourier transform infrared spectroscopy.The spontaneity and site-specificity of this unique reaction were confirmed via electrophoresis and fluorescence analyses.Although the alkaline stability of ST-GFP-ligated SC-SMBs was not ideal,the formed isopeptide bond was unbreakable under acidic conditions(0.05 M glycine-HCl buffer,pH 1e6)for 2 h,under 20%ethanol solution within 7 days,and at most temperatures.We,therefore,present a simple and universal strategy for the preparation of diverse protein-functionalized SMBs for ligand fishing,prompting its usage on drug screening and target finding. | Yu Yi Jianming Hu Shenwei Ding Jianfeng Mei Xudong Wang Yanlu Zhang Jianshu Chen Guoqing Ying | 2022 | Journal of Pharmaceutical Analysis2022，12，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员