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| 1 | Prevalence of Autism Spectrum Disorder in China:A Nationwide Multi-center Population-based Study Among Children Aged 6 to 12 Years显示文摘This study aimed to obtain the first national estimate of the prevalence of autism spectrum disorder(ASD) in Chinese children.We targeted the population of 6 to 12-year-old children for this prevalence study by multistage convenient cluster sampling.The Modified Chinese Autism Spectrum Rating Scale was used for the screening process.Of the target population of 142,086 children,88.5%(n=125,806) participated in the study.A total of 363 children were confirmed as having ASD.The observed ASD prevalence rate was 0.29%(95% CI:0.26%-0.32%) for the overall population.After adjustment for response rates,the estimated number of ASD cases was867 in the target population sample,thereby achieving an estimated prevalence of 0.70%(95% CI:0.64%-0.74%).The prevalence was significantly higher in boys than in girls(0.95%;95% CI:0.87%-1.02% versus 0.30%;95%CI:0.26%-0.34%;P <0.001).Of the 363 confirmed ASD cases,43.3% were newly diagnosed,and most of those(90.4%) were attending regular schools,and 68.8% of the children with ASD had at least one neuropsychiatric comorbidity.Our findings provide reliable data on the estimated ASD prevalence and comorbidities in Chinese children. | Hao Zhou Xiu Xu Weili Yan Xiaobing Zou Lijie Wu Xuerong Luo Tingyu Li Yi Huang Hongyan Guan Xiang Chen Meng Mao Kun Xia Lan Zhang Erzhen Li Xiaoling Ge Lili Zhang Chunpei Li Xudong Zhang Yuanfeng Zhou Ding Ding Andy Shih Eric Fombonne Yi Zheng Jisheng Han Zhongsheng Sun Yong-hui Jiang Yi Wang LATENT-NHC Study Team | 2020 | Neuroscience Bulletin2020，36，9： | 158 |
| 2 | Blockade of cholecystokinin-2 receptor and cyclooxygenase-2 synergistically induces cell apoptosis, and inhibits the proliferation of human gastric cancer cells in vitro显示文摘Gastrin and cyclooxygenase-2(COX-2) playimportant roles in the carcinogenesis and progression ofgastric cancer.However,it remains unknown whether the combination of cholecystokinin-2(CCK-2) receptor antagonist plus COX-2 inhibitor exerts synergistic anti-tumor effects on human gastric cancer.Here,we demonstrated that the combination of AG-041R(a CCK-2 receptor antagonist) plus NS-398(a selective COX-2 inhibitor) treatment had synergistic effects on proliferation inhibition,apoptosis induction,down-regulation of Bcl-2 and up-regulation of Bax expression in MKN-45 cells.These results indicate that simultaneous targeting of CCK-2 receptor and COX-2 may inhibit gastric cancer development more effectively than targeting either molecule alone.(C)2008 Elsevier Ireland Ltd.All rights reserved. | Sun, W. H. | 2008 | 南京医科大学学报(自然科学版)2008，28，9： | 30 |
| 3 | Clinical study on the treatment of liver fibrosis due to hepatitis B by IFN-α1 and traditional medicine preparation显示文摘 | CHENG Ming-Liang WU Ya-Yun HUANG Ke-Fu LUO Tian-Yong DING Yi-Shen LU Yin-Yin LIU RenCaiand Wu Jun | 1999 | World Journal of Gastroenterology1999，5，3： | 30 |
| 4 | PVT v2:Improved baselines with Pyramid Vision Transformer显示文摘Transformers have recently lead to encouraging progress in computer vision.In this work,we present new baselines by improving the original Pyramid Vision Transformer(PVT v1)by adding three designs:(i)a linear complexity attention layer,(ii)an overlapping patch embedding,and(iii)a convolutional feed-forward network.With these modifications,PVT v2 reduces the computational complexity of PVT v1 to linearity and provides significant improvements on fundamental vision tasks such as classification,detection,and segmentation.In particular,PVT v2 achieves comparable or better performance than recent work such as the Swin transformer.We hope this work will facilitate state-ofthe-art transformer research in computer vision.Code is available at http://gffzz188fe103f8f1460as0wv5fn9wb0vn65qp.ffgz.tsg.suse.edu.cn/whai362/PVT. | Wenhai Wang Enze Xie Xiang Li Deng-Ping Fan Kaitao Song Ding Liang Tong Lu Ping Luo Ling Shao | 2022 | Computational Visual Media2022，8，3： | 30 |
| 5 | Validity and reliability testing of the Chinese (mainland) version of the 39-item Parkinson's Disease Questionnaire (PDQ-39)显示文摘The 39-item Parkinson's Disease Questionnaire (PDQ-39) has been tested in many languages, but not in Chinese mainland. We aimed to assess the Chinese (mainland) version of the PDQ-39. Seventy-one subjects with Parkinson's disease (PD) completed the PDQ-39 and the Medical Outcomes Study 36-item Short Form Health Survey (SF-36). All subjects were retested with the PDQ-39 a week later. The united Parkinson's disease rating scale (UPDRS) and the Hoehn and Yahr (H & Y) scale were also used to evaluate the subjects. Reliability was assessed by Cron- bach's α and intra-class correlation coefficient (ICC). Validity was examined in terms of agreement with SF-36, UPDRS, and H & Y scales. The Chinese (mainland) version of the PDQ-39 demonstrated acceptable reliability (Cronbach's α: 0.84-0.88; ICC: 0.56-0.82). The item-total correlations (0.33-0.88) and scaling success rates (77.56%) indicated satisfactory convergent and discriminant validity of the PDQ-39 items. The correlations between related constructs of the PDQ-39 and UPDRS (r=0.44-0.68) and between those of the PDQ-39 and SF-36 (r=(-0.46)-(-0.69)) were all statistically significant (P<0.01). Except for stigma, cognitions, and bodily discomfort, all other dimensions of the PDQ-39 significantly discriminated patients at different H & Y stages indicated by the H & Y scale. Although our observations indicate that some problematic subscales of this version of the PDQ-39 could be improved upon, this study suggests acceptable reliability and validity of the Chinese (mainland) version of the PDQ-39. | Wei LUO Xiao-hong GUI Bo WANG Wen-ying ZHANG Zhi-yuan OUYANG Yi GUO Bao-rong ZHANG Mei-ping DING | 2010 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2010，11，7： | 25 |
| 6 | Antioxidative mechanism of Lycium barbarum polysaccharides promotes repair and regeneration following cavernous nerve injury显示文摘Polysaccharides extracted from Lycium barbarum exhibit antioxidant properties.We hypothesized that these polysaccharides resist oxidative stress-induced neuronal damage following cavernous nerve injury.In this study,rat models were intragastrically administered Lycium barbarum polysaccharides for 2 weeks at 1,7,and 14 days after cavernous nerve injury.Serum superoxide dismutase and glutathione peroxidase activities significantly increased at 1 and 2 weeks post-injury.Serum malondialdehyde levels decreased at 2 and 4 weeks.At 12 weeks,peak intracavernous pressure,the number of myelinated axons and nicotinamide adenine dinucleotide phosphate-diaphorase-positive nerve fibers,levels of phospho-endothelial nitric oxide synthase protein and 3-nitrotyrosine were higher in rats administered at 1 day post-injury compared with rats administered at 7 and 14 days post-injury.These findings suggest that application of Lycium barbarum polysaccharides following cavernous nerve crush injury effectively promotes nerve regeneration and erectile functional recovery.This neuroregenerative effect was most effective in rats orally administered Lycium barbarum polysaccharides at 1 day after cavernous nerve crush injury. | Zhan-kui Zhao Hong-lian Yu Bo Liu Hui Wang Qiong Luo Xie-gang Ding | 2016 | Neural Regeneration Research2016，11，8： | 25 |
| 7 | 食品中磷的检测方法显示文摘磷是人体内的重要元素之一,对人体生命活动有十分重要的作用,如何快速、简便、准确、灵敏地检测食品中磷的含量已经引起了世界各国的重视.本文总结了前人的研究工作,概述了近年来食品中总磷及有机磷农药的检测研究进展,分析了各种检测方法的优势和局限性,展望了未来该领域研究的发展趋势. | 卫敏 LIU RuiHai DING Liang CHENG WenLong XU JianFeng LUO Dan 刘钟栋 | 2010 | 中国科学:化学2010，40，7： | 24 |
| 8 | Effect of Substructure on Toughness of Lath Martensite/Bainite Mixed Structure in Low-Carbon Steels显示文摘The quantitative analysis of substructure in the martensite/bainite mixed structure, which is obtained from low-carbon NiCrMoV steels under different cooling conditions, was made by means of optical microscope (OM), scanning electron microscope (SEM), electron backscatter diffraction (EBSD), and transmission electron microscope (TEM), in order to research the effect on toughness. The test results indicate that the toughness of the steel is enhanced with the decrease in the packet and block size under the condition of the same prior austenite grain size mixed with different ratios of martensite and bainite while the lath width is about 0.38 μm. The calculation shows that both the packet and block boundaries have the same hindering effect on crack extension. Furthermore, the effect of the block width on impact energy is much larger than that of the packet. Therefore, the block can be used as microstructural substructure to affect the toughness in low-carbon martensite steels, suggesting that the block size is 'the effective grain size' for controlling toughness. | LUO Zhi-jun SHEN Jun-chang SU Hang DING Yue-hua YANG Cai-fu ZHU Xing | 2010 | Journal of Iron and Steel Research(International)2010，17，11： | 24 |
| 9 | Shikonin induces glioma cell necroptosis in vitro by ROS overproduction and promoting RIP1/RIP3 necrosome formation显示文摘 | Bin LU Xu GONG Zong-qi WANG Ye DING Chen WANG Tian-fei LUO Mei-hua PIAO Fan-kai MENG Guang-fan CHI Yi-nan LUO Peng-fei GE | 2017 | Acta Pharmacologica Sinica2017，38，11： | 22 |
| 10 | Effect of ultrasound-guided acupotomy vs electro-acupuncture on knee osteoarthritis:a randomized controlled study显示文摘OBJECTIVE:To investigate the effect of acupotomy on knee osteoarthritis(KOA),compared to electro-acupuncture(EA).METHODS:Sixty KOA patients were randomly divided into 2 groups:ultrasound-guided acupotomy group and EA group;each had 3 weeks' therapy.After the treatment,by contrast before and after therapy,by comparing curative effects among groups,we looked into disease improvement degree through activities of daily living score(ADL),hospital for special surgery index(HSS),visual analogue scales score(VAS) and knee joint's infrared thermal imaging detection.RESULTS:Graded by ADL,the excellent rate in acupotomy group was much higher than EA group;both acupotomy group and EA group had obviouschanges in HSS index before and after the therapy(P < 0.01).And there was remarkable difference in HSS index variation between the groups(P < 0.01).Acupotomy group and EA group showed big difference in pain index before and after treatment(P <0.01).Apparent difference also existed in the comparison among groups(P < 0.01).Both acupotomy group and EA group had apparent changes in infrared thermal imaging detection before and after the treatment(P < 0.01).CONCLUSION:Acupotomy and EA both have significant effects in KOA treatment;the former is better than the latter in relieving pain and improving knee functions. | Ding Yu Wang Yuexiang Shi Xian Luo Yun Gao Yuhong Pan Jingkun | 2016 | Journal of Traditional Chinese Medicine2016，36，4： | 21 |
| 11 | Effect of Y and Gd content on the microstructure and mechanical properties of Mg-Y-RE alloys显示文摘In the application of WE43,it is found that Y_(2)O_(3)inclusion formed in the process of casting seriously reduced the mechanical properties of the products.The reduction of the mechanical properties is even more distinct when it comes to the application in the thin walled complex-precision castings.In order to decrease the Y_(2)O_(3)inclusions,Gd element was used to replace part of the Y element in Mg-Y-RE series alloys.The effect of Y content(Mg-x Y-1Gd-2Nd-0.5Zn-0.5Zr)and Y/Gd ratio(Mg-x Y-(5-x)Gd-2Nd-0.5Zn-0.5Zr)on the microstructure and mechanical properties of Mg-Y-RE alloys were investigated in this paper.With decreasing Y content,the grain size of the alloys increased,both ultimate tensile strength(UTS)and yield strength(YS)of alloys decreased monotonically.Replacing part of Y content with Gd and keeping the total rare earth content unchanged,the low Y content Mg-2Y-3Gd-2Nd-0.5Zn-0.5Zr alloy showed the same mechanical properties as the high Y content Mg-4Y-1Gd-2Nd-0.5Zn-0.5Zr alloy at both room and elevated temperatures.After solution treatment at 525°C for 8 h and aging treatment at 225°C for 10 h,the UTS,YS and elongation(ε)of Mg-2Y-3Gd-2Nd-0.5Zn-0.5Zr alloy reached 281.7 MPa,198 MPa and 11.1%at room temperature,and 216.7 MPa,171.6 MPa and 16.1%at 250°C.The new low Y content Mg-2Y-2Nd-3Gd-0.5Zn-0.5Zr alloy is expected to replace the high Y content WE43 alloys,which can be used in the complex thin-walled parts of aviation products. | Kang Luo Liang Zhang Guohua Wu Wencai Liu Wenjiang Ding | 2019 | Journal of Magnesium and Alloys2019，7，2： | 20 |
| 12 | Value of gamma-glutamyltranspeptidase-to-platelet ratio in diagnosis of hepatic fibrosis in patients with chronic hepatitis B显示文摘AIM To investigate the value of the gamma-glutamyltraspeptidase(GGT)-to-platelet(PLT) ratio(GPR) in the diagnosis of hepatic fibrosis in patients with chronic hepatitis B(CHB). METHODS We included 390 untreated CHB patients in this study. The GPR, aspartate aminotransferase(AST)-to-PLT ratio index(APRI), and fibrosis-4(FIB-4) of all patients were analysed to determine if these parameter were correlated with age, gender, medical history, liver function [total bilirubin(TBil), alanine aminotransferase(ALT), and AST], GGT, PLT count, or hepatic fibrosis stage. The GPR, APRI, and FIB-4, as well as the combination of the GPR and APRI or the GPR and FIB-4 were assessed in different cirrhosis stages using receiver operating characteristic(ROC) curve analysis to evaluate their value in diagnosing hepatic fibrosis in CHB patients. RESULTS The GPR, APRI, and FIB-4 were not correlated withCHB patients' age, gender, or disease duration(P > 0.05), but all of these parameters were positively correlated with serum ALT, AST, GGT, and PLT count(P < 0.01). Additionally, the GPR, APRI, and FIB-4 were positively correlated with hepatic fibrosis(P < 0.01); the areas under the ROC curve for the GPR in F1, F2, F3, and F4 stages were 0.723, 0.741, 0.826, and 0.833, respectively, which were significantly higher than the respective values for the FIB-4 and APRI(F1: 0.581, 0.612; F2: 0.706, 0.711; F3: 0.73, 0.751; and F4: 0.799, 0.778). The respective diagnostic cut-off points for each stage were 0.402, 0.448, 0.548, and 0.833, respectively. The diagnostic sensitivity and specificity were, respectively, 88.8% and 87.5% in F1, 72.7% and 89.7% in F2, 81.3% and 98.6% in F3, and 80% and 97.4% in F4 when the GPR and APRI were connected in parallel; 86.6% and 90.2%, 78.4% and 96%, 78.6% and 97.4%, and 73.2% and 97.9%, respectively, when the GPR and APRI were connected in series; 80.2% and 89%, 65% and 89%, 70.3% and 98.5%, and 78.8% and 96.8%, respectively, when the GPR and FIB-4 were connected in parallel; and 83.6% and 87.9%, 76.8% and 96.6%, 72.7% and 98%, and 74.4% and 97.7%, respectively, when the GPR and FIB-4 were connected in series.CONCLUSION The GPR, as a serum diagnostic index of liver fibrosis, is more accurate, sensitive, and easy to use than the FIB-4 and APRI, and the GPR can significantly improve the sensitivity and specificity of hepatic fibrosis diagnosis in CHB when combined with the FIB-4 or APRI. | Yan-Chao Hu Hao Liu Xiao-Yan Liu Li-Na Ma Yu-Hua Guan Xia Luo Xiang-Chun Ding | 2017 | World Journal of Gastroenterology2017，23，41： | 19 |
| 13 | Incidence of infectious complications is associated with a high mortality in patients with hepatitis B virus-related acute-on-chronic liver failure显示文摘BACKGROUND In China,hepatitis B virus-related acute-on-chronic liver failure (HBV-ACLF) is the most common liver failure characterized by serious clinical syndromes of liver decompensation with a very high mortality.Bacterial and/or fungal infections are the most common complications that are associated with high short-term mortality.Bacterial translocation from the intestine,impaired hepatic clearance,and immune paralysis of circulating immune cells are thought to contribute to infectious complications in liver failure.The control of bacterial and fungal infections is the key to improving HBV-ACLF outcomes.Active prevention,early diagnosis,and timely treatment of bacterial and fungal infections are essential for treating HBV-ACLF.AIM To investigate the frequency and role of bacterial and fungal infections in patients with HBV-ACLF.METHODS Patients with HBV-ACLF hospitalized at Taihe Hospital,Hubei University of Medicine from January 2014 to December 2017 were retrospectively enrolled.Patient-related information was retrieved from the hospital case database,including general information,blood biochemistry,complications,etc.According to the occurrence of secondary infection or not,the patients were divided into an infection group and a non-infection group.The sites,types,and incidences of bacterial and fungal infections and the influence of infections on the prognosis of HBV-ACLF were statistically analyzed.The risk factors for infections were assessed by unconditional logistic regression.RESULTS There were 174 cases of HBV-ACLF that met the enrollment criteria,of which 114 (65.52%) were diagnosed with infectious complications.Infections occurred in the abdominal cavity (87 cases),respiratory tract (51 cases),urinary tract (18 cases),and biliary tract (10 cases).Patients with infectious complications had a significantly higher 28-d mortality (70.18%,80/114) than those without (40.00%,24/60)(70.18% vs 40.00%,P < 0.05).And patients with infectious complications had a much higher incidence of non-infectious complications (54.39%,62/114)(54.39% vs 15.00%,P < 0.05),leading to an extremely high 28-d mortality of 88.71%(55/62)(P < 0.05).The grade of liver failure,period of hospital stay ≥ 30 d,age ≥ 45 years,and percentage of neutrophils > 70% were identified as risk factors for infectious complications.CONCLUSION The high incidence of infectious complications in patients with HBV-ACLF is associated with severity and deterioration of the disease and may contribute to the extremely high mortality of these patients. | Chen Wang De-Qiang Ma Sen Luo Chuan-Min Wang De-Ping Ding You-You Tian Kang-Jian Ao Yin-Hua Zhang Yue Chen Zhong-Ji Meng | 2019 | World Journal of Clinical Cases2019，7，16： | 18 |
| 14 | High Potassium Aggravates the Oxidative Stress Inducedy by Magnesium Deficiency in Rice Leaves显示文摘Magnesium(Mg) deficiency in plant affects photosynthesis and many other metabolic processes.Rice(Oryza sativa L.cv.'Wuyunjing 7') plants were grown in hydroponics culture at three Mg and two potassium(K) levels under greenhouse conditions to examine the induction of oxidative stress and consequent antioxidant responses in rice leaves due to Mg deficiency.At low Mg(0.2 mmol L-1 Mg supply for two weeks after transplanting) and high K(6 mmol L-1) for 21 days,the rice plants showed severe Mg deficiency and a significant decreases in the dry matter production.The Mg deficiency in leaves decreased chlorophyll concentrations,photosynthetic activity,and soluble protein,but significantly increased the concentrations of soluble sugars and malondialdehyde(MDA) and the activities of superoxide dismutase(SOD,EC 1.15.1.1),catalase(CAT,EC 1.11.1.6) and peroxidase(POD,EC 1.11.1.7).In addition,Mg concentrations in the leaves and in the shoot biomass were negatively related to the activities of the three antioxidative enzymes and the concentration of MDA in leaves.There were very significant interactive effects between Mg and K supplied in the culture solution on shoot biomass yield,chlorophyll content,photosynthesis rate,the activities of SOD,CAT and POD,and MDA content in the leaves of rice.It is suggested that the high K level in the nutrient solution aggravated the effect of low Mg supply-induced Mg deficiency and created the oxidative damage in rice plants. | DING Yu-Chuan CHANG Chun-Rong LUO Wen WU Yan-Shou REN Xiao-Li WANG Ping XU Guo-Hua | 2008 | Pedosphere2008，18，3： | 17 |
| 15 | Prevalence, awareness, treatment, and control of hypertension in the non-dialysis chronic kidney disease patients显示文摘 | ZHENG Ying CAI Guang-yan CHEN Xiang-mei FU Ping CHEN Jiang-hua DING Xiao-qiang YU Xue-qing LIN Hong-li LIU Jian XIE Ru-juan WANG Li-ning NI Zhao-hui LIU Fu-you YIN Ai-ping XING Chang-ying WANG Li SHI Wei LIU Jian-she HE Ya-ni DING Guo-hua LI Wen-ge WU Guang-li MIAO Li-ning CHEN Nan SU Zhen MEI Chang-lin ZHAO Jiu-yang GU Yong BAI Yun-kai LUO Hui-min LIN Shan CHEN Meng-hua GONG Li YANG Yi-bin YANG Xiao-ping LI Ying WAN Jian-xin WANG Nian-song LI Hai-ying XI Chun-sheng HAO Li XU Yan FANG Jing-ai LIU Bi-cheng LI Rong-shan WANG Rong ZHANG Jing-hong WANG Jian-qin LOU Tan-qi SHAO Feng-min MEI Feng LIU Zhi-hong YUAN Wei-jie SUN Shi-ren ZHANG Ling ZHOU Chun-hua CHEN Qin-kai JIA Shun-lian GONG Zhi-feng GUAN Guang-ju XIA Tian ZHONG Liang-bao | 2013 | Chinese Medical Journal2013，，12： | 16 |
| 16 | Investigation on the broadband electromagnetic wave absorption properties and mechanism of Co3O4- nanosheets/reduced-graphene-oxide composite显示文摘cobaltosic-oxide-nanosheets/reduced-graphene-oxide 合成(CoNSs@RGO ) 成功地作为一个小宽带电磁波吸收器被准备。样品厚度的效果和数量合成在吸收上补充说性质彻底地被调查到石蜡样品。由于公司 3 O 4, 很高,波浪吸收材料的 surface-to-volume 比率在吸收性质正在导致大改进。为 3.6 公里的厚度的 CoNSs@RGO 合成 was-45.15 dB 的最大的茶点损失,和有思考损失 below-10 dB 的最好的吸收带宽是有 2.9 公里的厚度的 7.14 GHz。另外,微波吸收的山峰与增加吸收涂层的厚度向低频率区域变了。电磁波吸收的机制被归因于阻抗象 RGO 的绝缘的松驰和极化一样匹配 CoNSs@RGO。比作以前报导的吸收材料, CoNSs@RGO 在高频率乐队为申请作为一个无足轻重的人和高度有效的吸收材料显示出更好的性能。 | Yi Ding Zheng Zhang Baohe Luo Qingliang Liao Shuo Liu Yichong Liu Yue Zhang | 2017 | Nano Research2017，10，3： | 16 |
| 17 | Changes of Cu, Zn, and Cd speciation in sewage sludge during composting显示文摘The potential toxicity risks from heavy metals depend on their chemical speciation. The four stages of the Tessier sequential extraction method were employed to investigate changes in heavy metal speciation(Cu, Zn, and Cd) of sewage sludge during forced aeration composting, and then to identify whether the composting process would reduce or enhance their toxicities. Throughout the composting process, the exchangeable, carbonate-bound, Fe-Mn oxide-bound, and organic matter-bound fractions of Cu were converted to the residual Cu fraction. The organic matter-bound Cu fraction greatly contributed to this transformation. Residual Zn fraction was transformed to the Fe-Mn oxide-bound and organic matter-bound fractions after composting. The residual Zn fraction was a major contributor to the organic matter-bound Zn fraction. The availability of Cu and Zn was reduced by composting such that the risk of heavy metal toxicity decreased with prolonged treatment times. Additionally, attention should be paid to the increased availability of Cd in sewage sludge after composting treatment. | GAO Ding ZHENG Guo-di CHEN Tong-bin LUO Wei GAO Wei ZHANG Yi-an LI Yan-xia | 2005 | Journal of Environmental Sciences2005，17，6： | 15 |
| 18 | Promotion of spinosad biosynthesis by chromosomal integration of the Vitreoscilla hemoglobin gene in Saccharopolyspora spinosa显示文摘To promote spinosad biosynthesis by improving the limited oxygen supply during high-density fermentation of Saccharopolyspora spinosa, the open reading frame of the Vitreoscilla hemoglobin gene was placed under the control of the promoter for the erythromycin resistance gene by splicing using overlapping extension PCR. This was cloned into the integrating vector pSET152, yielding the Vitreoscilla hemoglobin gene expression plasmid pSET152EVHB. This was then introduced into S. spinosa SP06081 by conjugal transfer, and integrated into the chromosome by site-specific recombination at the integration site ΦC31 on pSET152EVHB. The resultant conjugant, S. spinosa S078-1101, was genetically stable. The integration was further confirmed by PCR and Southern blotting analysis. A carbon monoxide differential spectrum assay showed that active Vitreoscilla hemoglobin was successfully expressed in S. spinosa S078-1101. Fermentation results revealed that expression of the Vitreoscilla hemoglobin gene significantly promoted spinosad biosynthesis under normal oxygen and moderately oxygen-limiting conditions (P<0.01). These findings demonstrate that integrating expression of the Vitreoscilla hemoglobin gene improves oxygen uptake and is an effective means for the genetic improvement of S. spinosa fermentation. | LUO YuShuang KOU XiaoXiao DING XueZhi HU ShengBiao TANG Ying LI WenPing HUANG Fan YANG Qi CHEN HanNa XIA LiQiu | 2012 | Science China(Life Sciences)2012，55，2： | 14 |
| 19 | Detecting soil salinity with arid fraction integrated index and salinity index in feature space using Landsat TM imagery显示文摘Modeling soil salinity in an arid salt-affected ecosystem is a difficult task when using remote sensing data because of the complicated soil context(vegetation cover,moisture,surface roughness,and organic matter)and the weak spectral features of salinized soil.Therefore,an index such as the salinity index(SI)that only uses soil spectra may not detect soil salinity effectively and quantitatively.The use of vegetation reflectance as an indirect indicator can avoid limitations associated with the direct use of soil reflectance.The normalized difference vegetation index(NDVI),as the most common vegetation index,was found to be responsive to salinity but may not be available for retrieving sparse vegetation due to its sensitivity to background soil in arid areas.Therefore,the arid fraction integrated index(AFII)was created as supported by the spectral mixture analysis(SMA),which is more appropriate for analyzing variations in vegetation cover(particularly halophytes)than NDVI in the study area.Using soil and vegetation separately for detecting salinity perhaps is not feasible.Then,we developed a new and operational model,the soil salinity detecting model(SDM)that combines AFII and SI to quantitatively estimate the salt content in the surface soil.SDMs,including SDM1 and SDM2,were constructed through analyzing the spatial characteristics of soils with different salinization degree by integrating AFII and SI using a scatterplot.The SDMs were then compared to the combined spectral response index(COSRI)from field measurements with respect to the soil salt content.The results indicate that the SDM values are highly correlated with soil salinity,in contrast to the performance of COSRI.Strong exponential relationships were observed between soil salinity and SDMs(R2>0.86,RMSE<6.86)compared to COSRI(R2=0.71,RMSE=16.21).These results suggest that the feature space related to biophysical properties combined with AFII and SI can effectively provide information on soil salinity. | Fei WANG Xi CHEN GePing LUO JianLi DING XianFeng CHEN | 2013 | Journal of Arid Land2013，5，3： | 14 |
| 20 | miR-132 inhibits colorectal cancer invasion and metastasis via directly targeting ZEB2显示文摘AIM:To investigate the biological role and underlying mechanism of miR-132 in colorectal cancer(CRC)progression and invasion.METHODS:Quantitative RT-PCR analysis was used to examine the expression levels of miR-132 in five CRC cell lines(SW480,SW620,HCT116,HT29 and LoVo)and a normal colonic cell line NCM460,as well as in tumor tissues with or without metastases.The KaplanMeier method was used to analyze the prognostic significance of miR-132 in CRC patients.The biological effects of miR-132 were assessed in CRC cell lines using the transwell assay.Quantitative RT-PCR and western blot analyses were employed to evaluate the expression of miR-132 targets.The regulation of ZEB2 by miR-132was confirmed using the luciferase activity assay.RESULTS:miR-132 was significantly down-regulated in the CRC cell lines compared with the normal colonic cell line(P<0.05),as well as in the CRC tissues withdistant metastases compared with the tissues without metastases(10.52±4.69 vs 23.11±7.84)(P<0.001).Down-regulation of miR-132 was associated with tumor size(P=0.016),distant metastasis(P=0.002),and TNM stage(P=0.020)in CRC patients.Kaplan-Meier survival curve analysis indicated that patients with low expression of miR-132 tended to have worse diseasefree survival than patients with high expression of miR-132(P<0.001).Moreover,ectopic expression of miR-132 markedly inhibited cell invasion(P<0.05)and the epithelial-mesenchymal transition(EMT)in CRC cell lines.Further investigation revealed ZEB2,an EMT regulator,was a downstream target of miR-132.CONCLUSION:Our study indicated that miR-132 plays an important role in the invasion and metastasis of CRC. | Yong-Bin Zheng Hai-Ping Luo Qiang Shi Zhi-Nan Hao Yu Ding Qiu-Shuang Wang Sheng-Bo Li Gao-Chun Xiao Shi-Lun Tong | 2014 | World Journal of Gastroenterology2014，20，21： | 14 |
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