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| 1 | Quantitative analysis of vascular endothelial growth factor, microvascular density and their clinicopathologic features in human hepatocellular carcinoma显示文摘BACKGROUND: Angiogenesis is known to be essential to the survival, growth, invasion, and metastasis of tumor cells. Vascular endothelial growth factor (VEGF) are an important angiogenic factor regulating tumor angiogenesis, but its significance and tumor pathologic features are un- clear in hepatocellular carcinoma (HCC). In the present study, we analyzed expression of tissue VEGF, alteration of microvascular density (MVD) in microvessel angiogenesis, development and metastasis of HCC, and level of serum VEGF in differential diagnosis of benign and malignant liv- er diseases. METHODS: Tumor specimens were prospectively collected from HCC patients undergoing resection. Total RNAs were extracted and the expression levels were detected from different parts of HCC tissues. The cellular distributions of VEGF and MVD of liver tumors and their paracancerous and distal cancerous tissues were investigated by streptavi- din peroxidase (S-P) immunohistochemistry, respectively. The VEGF levels of circulating blood and hepatoma tissues were measured by enzyme-linked immunosorbent assay. RESULTS: The incidence of VEGF expression was 63.9% in HCCs (23/36 cases), 78.3% in non-encapsulated HCCs (18/23), and 90.9% in HCCs with extrahepatic metastasis (10/11), respectively. The VEGF expression was tightly correlated with MVD (P <0.01). The MVD in HCC with metastasis, low differentiation or non-encapsulation was significantly higher than that in HCC with intact capsule, high differentiation, or no metastasis. No significant diffe- rence was found between VEGF, MVD, tumor size, and hepatitis virus infection. The level of total RNA in HCC tis- sues was significantly lower but the VEGF level significantly higher than those in paracancerous or distal cancerous ones (P<0.01). The abnormal expression levels of VEGF in sera of HCC patients were directly correlated with the me- tastasis and recurrence of tumors. CONCLUSION: The high expression of VEGF and abnor- mality of tissue MVD are useful predictors for vascular inva- sion and metastasis of liver tumors. | Deng-Fu Yao, Xin-Hua Wu, Yong Zhu, Gong-Sheng Shi, Zhi-Zhen Dong, Deng-Bing Yao, Wei Wu, Li-Wei Qiu and Xian-Yong Meng Nantong, China Research Center of Clinical Molecular Biology , Department of Pathology and Department of Gastroenterology , Affiliated Hos- pital of Nantong University Department of Diagnostics , and Institute of Neurosciences , Nantong University Nantong 226001, China | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，2： | 81 |
| 2 | Clinical impact of plasma TGF-β1 and circulating TGF-β1 mRNA in diagnosis of hepatocellular carcinoma显示文摘BACKGROUND:Transforming growth factor-β(TGF-β) plays an important role in the regulation of cell growth and differentiation,angiogenesis,extracellular matrix formation,immunosuppression and cancer development. In this study,we investigated the levels of TGF-β1 and TGF-β1 mRNA expression,their relationship with HBV replication,and their diagnostic value for hepatocellular carcinoma(HCC). METHODS:Total RNAs were extracted from HCC samples and matched non-tumor tissues,and from peripheral blood mononuclear cells in HCC patients.TGF-β1 mRNA was amplified by RT-PCR and confirmed by DNA sequencing. The distribution of TGF-β1 expression was assessed by immunohistochemistry.The clinical characteristics were analyzed between TGF-β1 and HBV replication.The diagnostic value of circulating TGF-β1 and TGF-β1 mRNA levels were investigated in HCC patients. RESULTS:The incidence of hepatic TGF-β1 expression was 83.3%in HCC samples,43.3%in the surrounding tissues, 94.7%in the HBV DNA-positive group,and 63.6%in the HBV DNA-negative group.Liver TGF-β1 expression was associated with the degree of HCC differentiation and the status of HBV replication,but not with the size or number of tumors.Circulating TGF-β1 level and incidence of TGF-β1 mRNA were significantly higher in the HCC groupthan in any group of patients with benign liver disease, with a higher sensitivity of 89.5%and a specificity of 94.0% for HCC diagnosis when circulating TGF-β1 levels were >1.2μg/L.No significant correlation was found between TGF-β1 expression and AFP level or tumor size.Combining TGF-β1 level and serum AFP raised the detection rate to 97.4%. CONCLUSIONS:Abnormal expression of hepatic TGF-β1 is associated with the degree of HCC differentiation and HBV replication.Both circulating TGF-β1 and TGF-β1 mRNA can be used as sensitive biomarkers for the diagnosis and prognosis of HBV-induced HCC. | Zhi-Zhen Dong,Deng-Fu Yao,Min Yao,Li-Wei Qiu,Lei Zong,Wei Wu, Xin-Hua Wu,Deng-Bing Yao and Xian-Yong Meng Department of Diagnostics,Research Center of Clinical Molecular Biology,Department of Laboratory Science,and Department of GastroenterologyAffiliated Hospital and Institute of Neurosciences,Nantong University,Nantong 226001,China | 2008 | Hepatobiliary & Pancreatic Diseases International2008，7，3： | 27 |
| 3 | Immune Responses to the Attenuated Mycoplasma hyopneumoniae 168 Strain Vaccine by Intrapulmonic Immunization in Piglets显示文摘To investigate the immune responses to the attenuated Mycoplasma hyopneumoniae 168 strain vaccine, 8-15 d old piglets were immunized with M. hyopneumoniae 168 strain vaccine by intrapulmonic route. And the specific IgG antibody in serum, lymphoproliferation, IFNγ, and specific secretory IgA (SIgA) antibody in bronchoalveolar lavage fluid were detected on 30 and 60 d post-immunization (DPI), respectively. On 60 DPI, all the pigs except for those in health control group were challenged with a field M. hyopneumoniae strain JS. Necropsy was performed on 30 d post-challenge (DPC). The results showed that IFNγ and specific SIgA were stimulated on surface of respiratory tract after immunization. And peripheral blood mononuclear cells could also be proliferated about 1.81 and 2.12 fold on 30 and 60 DPI when stimulated by M. hyopneumoniae protein in vitro. However, no serum IgG antibody against M. hyopneumoniae was detected during the whole immune phage. After challenge, vaccinated pigs were observed with only very slight histological lesion in individual lobes. None of vaccinated pigs showed any clinical signs. While the unvaccinated pigs from challenge control group showed varying degrees of clinical sign and severe macroscopical lesion of mycoplasmal pneumonia of swine (MPS). The result suggested that the attenuated M. hyopneumoniae 168 strain vaccine inoculated by intrapulmonic route could activate the systemic cellular immunity, the local mucosal immunity and IFNγ secretion in respiratory tract to against M. hyopneumoniae infection in piglets. | FENG Zhi-xin, SHAO Guo-qing, LIU Mao-jun, WU Xu-su, ZHOU Yong-qi and GAN Yuan Key Laboratory of Animal Diseases Diagnostic and Immunology, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences/National Center for Engineering Research of Veterinary Bio-Products, Ministry of Agriculture, Nanjing 210014, P.R.China | 2010 | Agricultural Sciences in China2010，9，3： | 22 |
| 4 | Complete nucleotide sequence of a novel porcine circovirus-like agent and its infectivity in vitro显示文摘A novel agent (hence termed as P2) was isolated from pig sera in China, which contained covalently bound circular genomic DNAs of 993 nucleotides. Sequence analyses indicated that the agent was closely related to the porcine circovirus (PCV). The molecular clone of P2 was constructed subsequently and used for the following studies. Intracytoplasmic inclusions and intranuclear inclusions were only found in PK-15 cells transfected with the tandem dimer of P2 molecular DNA clone. Intracytoplasmic inclusions were round or irregular in shape and 0.1-0.4 μm in diameter, and intranuclear inclusions were electronically denser than intracytoplasmic inclusions and had two general shapes: round/small (0.1 μm in diameter) and hexagonal/large (0.5―1.4 μm in diameter). The inclusions were not membranously bound. The cells transfected with the tandem dimer of P2 molecular DNA clone were tested positive for P2 DNA at passages 5. The P2 antigen could be detected in both transfected and passaged PK-15 cells. This is the first report regarding the complete nucleotide sequence of a small DNA genome in a circovirus-like infectious agent in vitro. | WEN LiBin1, HE KongWang1, YANG HanChun2, NI YanXiu1, ZHANG XueHan1, GUO RongLi1 & PAN QunXin1 1 Key Laboratory of Animal and Poultry Diseases Diagnostic of the Ministry of Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China 2 Key Laboratory of Preventive Veterinary Medicine of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China | 2008 | Science China(Life Sciences)2008，51，5： | 14 |
| 5 | 骨岛(内生骨疣)的影像学和病理学特征回顾显示文摘骨岛(内生骨疣)的影像学和病理学特征回顾AdamGreenspan,M.D.BoneIsland(Enostosis):AReviewofImagingandPathologicCharacteristics￥AdamGreenspan,M.D.De... | Adam Greenspan,M.D.Department of Diagnostic Radiology,(University of California,Davis Medical Center 2516 Stockton Blvd.,Ticon Ⅱ Sacramento,CA 95817,U.S.A.) | 1996 | 临床放射学杂志1996，15，2： | 10 |
| 6 | Analysis of HLA alleles polymorphism in Chinese patients with primary biliary cirrhosis显示文摘BACKGROUND: A familial dustering of patients with primary biliary cirrhosis (PBC) and the presence of immunological abnormalities in family members suggest a genetic component involved in the pathogenesis of PBC. The aims of this study are to investigate the frequencies of human leukocyte antigen HLA-A, -B, and -DRB1 alleles in Chinese patients with PBC by polymerase chain reaction (PCR)-based techniques, and to assess the correlation of the above-mentioned HLA with some clinical and laboratory features. METHODS: Genotyping of HLA alleles were performed in 65 well-characterized PBC patients and 431 healthy controls with sequence-specifc primers PCR amplification. RESULTS: HLA-DRB1~*07 allele detected in 19 of the 65 (29.2%) PBC patients was subtyped as DRB1~*0701, as well as in 13.9% of controls (P_C<0.05, OR=2.55, 95% CI: 1.4-4.6). An increased frequency of DRB1~*03 (18.4% vs. 7.2% in healthy controls) and a decreased frequency of DRB1~*12 (16.9% vs. 28.8%) in PBC patients were statistically significant. There was no association with HLADRB1~*08 reported. The frequencies for HLA-A, B and the other DRB1 alleles were similar between patients and healthy controls. CONCLUSIONS: The susceptibility to PBC in Chinese individuals is associated with DRB1~*0701 allele. This association differs from that in North Americans, South Americans, North Europeans and even Japanese, but it is not restricted to any particular subgroup of patients. | Hai-Ying Liu. An-Mei Deng, Ye Zhou, Ding-Kang Yao, De-Xing Xu and Ren-Qian Zhong Clinical Laboratory, General Hospital of Guangzhou Military Command of PLA, Guangzhou 510010, China Laboratory Diagnostics, Changzheng Hospital, Second Military Medical University, and Clinical Immunology Center of PLA, Shanghai 200003, China and Department of Gastroenterology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China | 2006 | Hepatobiliary & Pancreatic Diseases International2006，5，1： | 9 |
| 7 | The relationship between HBV lamivudine resistance and HBV genotypes or basic core promoter mutations显示文摘OBJECTIVE: To investigate the relationship between HBV Iamivudine resistance and HBV genotypes orbasic core promoter (BCP) mutations.METHODS: The common coated probes were synthesized according to the conserved regions of the preCgene of hepatitis B virus (HBV). Different colorized probes were chosen from the sequences of differentgenotypes of HBV (A to F), BCP and YMDD wild types and mutants, respectively. HBV DNA levels,HBV genotypes, BCP and YMDD resistants were analyzed by PCR microplate hybridization ELISA at thezero and 6th month after the patients were treated with lamivudine.RESULTS: HBV genotyping results showed that HBV types B, C, D accounted for about 30%, 36%and 23% patients respectively. Thirteen BCP mutations (type B in 1 patient, type C in 8 and type D in4) were found before treatment with lamivudine. HBV DNA levels were lower than 100 pg/ml in 2patients anti higher than 100 pg/ml in 11. 9.4% of the HBV patients (5/43; type C in 3 and type D in2) showed YMDD resistants and 4 BCP mutations at the same time.CONCLUSION: Oral treatment of lamivudine decreases the level of serum HBV DNA. The appearanceof HBV YMDD resistants is related to certain HBV genotypes, and most of them are BCP mutations. | Dong-Yao Zhou Lian-Ying Lin Hong Wang Jian-Sheng Huang the Department of Clinical Laboratory Medicine, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510120, China Molecular Biology Diagnostic Center, First Military Medical University, Guangzhou 510515, China | 2003 | Hepatobiliary & Pancreatic Diseases International2003，2，1： | 5 |
| 8 | Radioimmunoimaging of colorectal cancer using ^(99m)Tc-labeled monoclonal antibody显示文摘Radioimmunoimagingofcolorectalcancerusing99mTclabeledmonoclonalantibodyHUJinYue1,SUJiaanZhi2,PIZhiMing3,ZHUJianGao1,ZHO... | HU JinYue1, SU JianZhi2, PI ZhiMing3, ZHU JianGao1, ZHOU GuoHua1 and SUN QuBing1Keywords colorectal neoplasms/radioimmunoimaging antibody, monoclonal isotope labeling technium/diagnostic use | 1998 | World Journal of Gastroenterology1998，4，4： | 4 |
| 9 | Somatostatin-Probably the most widely effective gastrointestinal hormone in human body显示文摘Somatostatin-ProbablythemostwidelyefectivegastrointestinalhormoneinhumanbodyHUANGXiangQianSubjectHeadingssomatostatin/physio... | HUANG Xiang Qian Keywords somatostatin/physiology somatostatin/analogs and derivatives receptors, somatostatin octreotide/diagnostic use octreotide/therapeutic use | 1997 | World Journal of Gastroenterology1997，3，4： | 4 |
| 10 | HBV resistant to lamivudine: experimental and clinical studies显示文摘Objective: To identify the impact of lamivudine onHBV e antigen (HBeAg) seroconversion and HBVDNA level, and the appearance of Tyr-Met-Asn-Asp(YMDD) resistants.Methods: Forty-seven hepatitis B patients were trea-ted with oral lamivudine. ALT level and HBeAgwere detected in the treatment on the zero, 3rd, 6thand 9th month respectively. The levels of HBV DNAand YMDD resistants were analyzed with PCR mi-croplate hybridization-ELISA.Results: After 9 months of treatment, HBV DNA be-came negative and ALT level was normal in 74% pa-tients. Among these patients, 17% patients hadHBeAg converted to negative and anti-HBe antibodypositive, whereas another 15% patients showedHBeAg negative. YMDD resistants appeared in 19 %patients (9/47). One, three and five resistants weredetected in the treatment on the 3rd, 6th and 9thmonth respectively.Conclusions: Most HBV DNA in serum became nega-tive after 9 months of treatment, and the rate ofHBeAg seroconversion was 17% (HBV DNA levelwas lower than 100 pg/ml before treatment). YMDDresistants appeared in 19% patients. | Dong-Yao Zhou Yong-Jian Cao Lian-Ying Lin Hong Wang Jian-Sheng Huang the Department of Clinical Laboratory Medicine, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510120, China Institution of Molecular Biology Diagnostic Center, First Military Medical University, Guangzhou 510515, China | 2002 | Hepatobiliary & Pancreatic Diseases International2002，1，4： | 3 |
| 11 | syndromes显示文摘 | Quilici j Banzet N Paule P Circulating endothelial cell count as a diagnostic marker for non-ST-elevation acute coronary | 2004 | Circulation2004，110，10： | 1 |
| 12 | The European society for pediatric gastroenterology and nutrition working group for the diagnostic criteria for food allergy显示文摘 | Diagnostic criteria for food allergy with predominantly intestinal symptoms | 1992 | J Pediatr Gastroenterol Nutr1992，14，： | 1 |
| 13 | Preliminary criteria for the classification of systemic sclerosis (scleroderma) 显示文摘 | Subcommittee for scleroderma criteria of the American Rheumatism Association Diagnostic and Therapeutic Criteria Committee | 1980 | Arthritis Rheum1980，23，： | 1 |
| 14 | measurement of electron temperatures and electron energy distribution functions显示文摘 | Malyshev M V Donnelly V M Diagnostics of cholorine inductively coupled plasmas | | 0，，： | 1 |
| 15 | Methodologies to Diagnose and Monitor Dry Eye Disease: Report of the Diagnostic Methodology Subcommittee of the International Dry Eye WorkShop (2007) 显示文摘 | Diagnostic Methodology Subcommittee members | 2007 | The Ocular Surface2007，5，2： | 1 |
| 16 | Preliminary criteria for the classification of systemic sclerosis显示文摘 | Subcommittee for Scleroderma Criteria of the American Rheuma-tism Association Diagnostic and Therapeutic Criteria Committee | 1980 | Arthritis Rheum1980，23，： | 1 |
| 17 | Fetal soft markers in obstetric ultrasound显示文摘 | Van den Hof MC Wilson RD Diagnostic Imaging Committee etal | 2005 | J Obstet GynaecolCan2005，27，6： | 1 |
| 18 | Preliminary criteria for the classification ofsystemic sclerosis (scleroderma) 显示文摘 | Subcommittee for Sclerederma Criterial of the American Rheumatism Association Diagnostic and Therapeutic Criteria Committee | 1983 | Arthritis Rheum1983，，26： | 1 |
| 19 | Cranial neuralgias and facial pain显示文摘 | Headache Classification Committee of the International Headache Headache Society Classification and Diagnostic Criteria for Headache Disorders | 1988 | Cephalalgia1988，8，7： | 1 |
| 20 | Fetal soft markers in obstetric ultrasound显示文摘 | Van den Hof MC Wilson RD Diagnostic Imaging Committee | 2005 | J Obstet Gynaecol Can2005，27，6： | 1 |
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