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| 1 | Bacterial presence on flexible endoscopes vs time since disinfection显示文摘AIM To correlate the length of endoscope hang time and number of bacteria cultured prior to use.METHODS Prospectively, we cultured specimens from 19 gastroscopes, 24 colonoscopes and 5 side viewing duodenoscopes during the period of 2011 to 2015. A total of 164 results had complete data denoting date of cleansing, number of days stored and culture results. All scopes underwent initial cleaning in the endoscopy suite utilizing tap water, and then manually cleaned and flushed. High level disinfection was achieved with a Medivator~? DSD(Medivator Inc., United States) automated endoscope reprocessor following manufacturer instructions, with Glutacide~?(Pharmax Limited, Canada), a 2% glutaraldehyde solution. After disinfection, all scopes were stored in dust free, unfiltered commercial cabinets for up to 7 d. Prior to use, all scopes were sampled and plated on sheep blood agar for 48 h; the colony count was obtained from each plate. The length of endoscope hang time and bacterial load was analyzed utilizing unpaired t-tests. The overall percentage of positive and negative cultures for each type of endoscope was also calculated. RESULTS All culture results were within the acceptable range(less than 200 cfu/mL). One colonoscope cultured 80 cfu/mL after hanging for 1 d, which was the highest count. ERCP scopes cultured at most 10 cfu, this occurred after 2 and 7 d, and gastroscopes cultured 50 cfu/mL at most, at 1 d. Most cultures were negative for growth, irrespective of the length of hang time. Furthermore, all scopes, with the exception of one colonoscope which had two positive cultures(each of 10 cfu/mL), had at most one positive culture. There was no significant difference in the number of bacteria cultured after 1 d compared to 7 d when all scopes were combined(day 2: P = 0.515; day 3: P = identical; day 4: P = 0.071; day 5: P = 0.470; day 6: P = 0.584; day 7: P = 0.575). There was also no significant difference in the number of bacteria cultured after 1 day compared to 7 d for gastroscopes(day 2: P = 0.895; day 3: P = identical; day 4: P = identical; day 5: P = 0.893; day 6: P = identical; day 7: P = 0.756), colonoscopes(day 2: P = 0.489; day 4: P = 0.493; day 5: P = 0.324; day 6: P = 0.526; day 7: P = identical), or ERCP scopes(day 2: P = identical; day 7: P = 0.685). CONCLUSION There is no correlation between hang time and bacterial load. Endoscopes do not need to be reprocessed if reused within a period of 7 d. | Katlin I Mallette Peter Pieroni Sonny S Dhalla | 2018 | World Journal of Gastrointestinal Endoscopy2018,10,1: | 3 |
| 2 | Role of oxidative stress in cardiovascular diseases显示文摘 | Naranjan S. Dhalla Rana M. Temsah Thomas Netticadan | 2000 | Journal of Hypertension2000,,6: | 2 |
| 3 | Alterations in heart sarcolemmal Ca2+-ATPase and Ca2+-binding activities due to oxygen free radicals显示文摘 | M. Kaneko P. K. Singal Dr. N. S. Dhalla | 1990 | Basic Research in Cardiology1990,,1: | 2 |
| 4 | Status of myocardial antioxidants in ischemia-reperfusion injury显示文摘 | Dhalla N S Elmoselhi A B Hata T | 2000 | Cardiovasc Res2000,47,3: | 1 |
| 5 | Ischemia-reperfusion induced changes in sarcolemmal Na^+/K^+-ATPase are due to the activation of calpain in the heart显示文摘 | Singh RB Dhalla NS | 2010 | Can J Physiol Pharmacol2010,88,3: | 1 |
| 6 | Role of oxidative stress in cardiovascular diseases显示文摘 | Dhalla NS Temsah RM Netticadan T | 2000 | J Hypertens2000,18,6: | 1 |
| 7 | Subcellular remodelling may induce cardiac dysfunction in congestive heart failure显示文摘 | Dhalla NS Saini-Chohan HK Rodriguez-Leyva D | | Cardiovascular Research0,,: | 1 |
| 8 | Angiogenesis-a new target for future therapy显示文摘 | Pandya NM Dhalla NS Santani DD | 2006 | Vascul Phamacol2006,44,5: | 1 |
| 9 | Partial prevention of changes in SR gene expression in congestive heart failure due to myocardial infarction by enalapril or losartan显示文摘 | Guo X Chapman D Dhalla NS | 2003 | Mol Cell Biochem2003,254,12: | 1 |
| 10 | Role of H202 in changing beta-adrenoceptor and adenylyl cyclase in ischmia-reperfused hearts显示文摘 | Dhalla NS | 1998 | Mol Cell Biochem1998,186,: | 1 |
| 11 | Subcellular remodeling as a viable target for the treatment of congestive heart failure显示文摘 | Dhalla NS Dent MR Tappia PS | 2006 | J Cardiovasc Pharmacol Therapeut2006,11,1: | 1 |
| 12 | Modification of beta adrenoceptor signal transduction pathway by genetic manipulation and heart failure显示文摘 | WANG X DHALLA N S | 2000 | Mol Cell Biochem2000,214,12: | 1 |
| 13 | Angiogenesis-a new target for future therapy 显示文摘 | Pandya NM Dhalla NS Santani DD | 2006 | Vascul Pharmacol2006,44,5: | 1 |
| 14 | Retinal pigment epithelial tear following intravitreal pegaptanib sodium 显示文摘 | Dhalla MS Blinder K J Tewari A | 2006 | Am J Ophthalmol2006,142,4: | 1 |
| 15 | Development of cardiac sensitivity to oxygen deficiency: comparative and ontogenetic aspects 显示文摘 | Ostadal B Ostadalova I Dhalla NS | 1999 | physiol Rev1999,79,3: | 1 |
| 16 | Role of oxidative stress in transition of hypertrophy to heart failure显示文摘 | Hill MF Singal PK | 1996 | J Am Coll Cardiol1996,28,2: | 1 |
| 17 | Role of oxidative stress in alterations of mitochondrial function in ischemic-reperfused hearts显示文摘 | Makazan Z Saini H K Dhalla N S | 2007 | Am J Physiol Heart Circ Physiol2007,292,: | 1 |
| 18 | Blood flow velocity quantification using split-spectrum amplitude decorrelation angiography with optical coherence tomography 显示文摘 | Tokayer J Jia Y Dhalla AH | 2013 | Biomed Opt Express2013,4,10: | 1 |
| 19 | Subcellular remodelling may induce cardiac dysfunctionin congestive heart failure显示文摘 | Dhalla N S Saini-Chohan H K Rodriguez-Leyva D | 2009 | Cardiovasc Res2009,81,3: | 1 |
| 20 | Potential of endothelin-1 and vasopressin antagonists for the treatment of congestive heart failure显示文摘 | Rehsia NS Dhalla N | 2010 | Heart Fail Rev2010,15,1: | 1 |