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| 1 | 印尼热泉中产嗜热碱性蛋白酶菌株筛选及酶学性质研究显示文摘采用MSM寡营养培养基从印度尼西亚Pantai cermin,Kalianda和Banyuwedang三个地区的热泉水样、泥样以及沉积物样品中分离获得细菌菌株,通过检测蛋白酶产生透明圈和福林酚蛋白酶活性测定相结合的方法,从中筛选出1株产嗜热蛋白酶的菌株PBI,该菌株经初步鉴定为短杆菌属(Brevibacillus),并对其酶学性质进行了研究。结果表明,菌株PBI产蛋白酶的最适酶活温度为60℃,最适pH值为8.0~9.0,具有较好的热稳定性和pH稳定性,60℃时酶的半衰期为30min,70℃条件下20min仍保持46.1%的酶活,该酶在pH值为5.0~9.0范围的缓冲液中酶活相对稳定。其产嗜热蛋白酶的酶活力最高可达到60.53U/mL,在100℃条件下仍能保留26.37%的相对酶活。Fe2+,Fe3+,Cu2+和Zn2+对嗜热蛋白酶活性具有明显的抑制作用。该嗜热蛋白酶对EDTA敏感,苯甲基磺酰氟(PMSF)、亮抑酶肽(Leupeptin)、苄眯(Benzamidine)和胃蛋白酶抑制剂(Pepstain A)对该嗜热蛋白酶都有一定的抑制作用,说明其酶活性受到丝氨酸、半胱氨酸残基的影响。结果表明,该菌株是1株具有进一步改造利用价值的产蛋白酶菌株。 | 王帅 林学政 黄晓航 郑立 ZILDA Dewi Seswita | 2012 | 海洋科学进展2012，30，2： | 8 |
| 2 | Hesperidin as a preventive resistance agent in MCF-7 breast cancer cells line resistance to doxorubicin显示文摘Objective:To evaluate of hesperidin to overcome resistance of doxorubicin in MCF-7 resistant doxorubicin cells(MCF-7/Dox)in cytotoxicity apoptosis and P-glycoprotein(Pgp)expression in combination with doxorubicin.Methods:The cytotoxic properties.50%inhibition concentration(IC_(50))and its combination with doxorubicin in MCF-7 cell lines resistant to doxorubicin(MCF-7/Dox)cells were determined using MTT assay.Apoptosis induction was examined by double staining assay using ethidium bromide-acridine orange.Immunocytochemistry assay was performed to determine the level and localization of Pgp.Results:Single treatment of hesperidin showed cytotoxic activity on MCF-7/Dox cells with IC_(50)value of 11μmol/L.Thus,combination treatment from hesperidin and doxorubicin showed addictive and antagonist effect(CI>1.0).Hesperidin did not increase the apoptotic induction,but decreased the Pgp expressions level when combined with doxorubicin in low concentration.Conclusions:Hesperidin has cytotoxic effect on MCF-7/Dox cells with IC_(50)of 11μmol/L.Hesperidin did not increased the apoptotic induction combined with doxorubicin.Cochemotherapy application of doxorubicin and hesperidin on MCF-7/Dox cells showed synergism effect through inhibition of Pgp expression. | Rifki Febriansah Dyaningtyas Dewi P.P. Sarmoko Nunuk Aries Nurulita Edy Meiyanto Agung Endro Nugroho | 2014 | Asian Pacific Journal of Tropical Biomedicine2014，4，3： | 5 |
| 3 | Tetramethylpyrazine stimulates cystic fibrosis transmembrane conductance regulator-mediated anion secretion in distal colon of rodents显示文摘AIM: To investigate the effect of tetramethylpyrazine (TMP), an active compound from Ligustiun Wollichii Franchat, on electrolyte transport across the distal colon of rodents and the mechanism involved.METHODS: The short-circuit current (ISC) technique in conjunction with pharmacological agents and specific inhibitors were used in analyzing the electrolyte transport across the distal colon of rodents. The underlying cellular signaling mechanism was investigated by radioimmunoassay analysis (RIA) and a special mouse model of cystic fibrosis.RESULTS: TMP stimulated a concentration-dependent rise in ISC, which was dependent on both Cl- and HCO3-, and inhibited by apical application of diphenylamine-2,2'-dicarboxylic acid (DPC) and glibenclamide, but resistant to 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid disodium salt hydrate (DIDS). Removal of Na+ from basolateral solution almost completely abolished the ISC response to TMP, but it was insensitive to apical Na+ replacement or apical Na+channel blocker, amiloride. Pretreatment of colonic mucosa with BAPTA-AM, a membrane-permeable selective Ca2+chelator, did not significantly alter the TMP-induced ISC. No additive effect of forskolin and 3-isobutyl-1-methylxanthine (IBMX) was observed on the TMP-induced ISc, but it was significantly reduced by a protein kinase A inhibitor, H89.RIA results showed that TMP (1 mmol/L) elicited a significant increase in cellular cAMP production, which was similar to that elicited by the adenylate cyclase activator, forskolin (10 μmol/L). The TMP-elicited ISC as well as forskolin- or IBMX-induced ISC were abolished in mice with homozygous mutation of the cystic fibrosis transmembrane conductance regulator (CFTR) presenting defective CFTR functions and secretions.CONCLUSION: TMP may stimulate cAMP-dependent and CFTR-mediated Cl- and HCO3- secretion. This may have implications in the future development of alternative treatment for constipation. | Qiong He Jin-Xia Zhu Ying Xing Lai-Ling Tsang Ning Yang Dewi Kenneth Rowlands Yiu-Wa Chung Hsiao-Chang Chan | 2005 | World Journal of Gastroenterology2005，11，27： | 4 |
| 4 | Antioxidant and α-glucosidase inhibitor activities of natural compounds isolated from Quercus gilva Blume leaves显示文摘Objective: To isolate and investigate antioxidant and α-glucosidase inhibitor compounds in the leaves of Quercus gilva Blume(Q. gilva).Methods: Dry leaves of Q. gilva were extracted with methanol and the methanolic extract was further separated by silica gel column chromatography using several solvents with increasing polarity. The antioxidant activities of the isolated compounds were evaluated using various in vitro assays: 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, hydrogen peroxide radical scavenging activity, β-carotene bleaching assay, and reducing power assay. The α-glucosidase inhibitory assay was conducted against α-glucosidase from Saccharomyces cerevisiae.Results: Three compounds were isolated and their structures were identii ed as catechin(1), epicatechin(2), and tiliroside(3) using an instrumental analysis. Compound 2 had higher antioxidant activity with inhibitory concentrations(IC50) of(22.55 ± 2.23) μmol/L than that of quercetin, which was used as the standard, with an IC50 of(28.08 ± 2.39) μmol/L, followed by compound 1 with IC50 of(40.86 ± 3.45) μmol/L. On the other hand, compound 3 had the lowest antioxidant activity with an IC50 of(160.24 ± 8.15) μmol/L. However, compound 3 had the highest α-glucosidase inhibitory activity with an IC50 of(28.36 ± 0.11) μmol/L, followed by compounds 1 and 2 with(168.60 ± 5.15) and(920.60 ± 10.10) μmol/L, respectively.Conclusions: The results obtained for the antioxidant activities and α-glucosidase inhibitory activities in a methanolic extract from the leaves of Q. gilva coni rmed the potential of this plant as a source of natural antioxidants and antidiabetic medicine. | Anastasia Wheni Indrianingsih Sanro Tachibana Rizna Triana Dewi Kazutaka Itoh | 2015 | Asian Pacific Journal of Tropical Biomedicine2015，5，9： | 4 |
| 5 | Chemopreventive effect of apple and berry fruits against colon cancer显示文摘Colon cancer arises due to the conversion of precancerous polyps(benign)found in the inner lining of the colon.Prevention is better than cure,and this is very true with respect to colon cancer.Various epidemiologic studies have linked colorectal cancer with food intake.Apple and berry juices are widely consumed among various ethnicities because of their nutritious values.In this review article,chemopreventive effects of these fruit juices against colon cancer are discussed.Studies dealing with bioavailability,in vitro and in vivo effects of apple and berry juices are emphasized in this article.A thorough literature survey indicated that various phenolic phytochemicals present in these fruit juices have the innate potential to inhibit colon cancer cell lines.This review proposes the need for more preclinical evidence for the effects of fruit juices against different colon cancer cells,and also strives to facilitate clinical studies using these juices in humans in large trials.The conclusion of the review is that these apple and berry juices will be possible candidates in the campaign against colon cancer. | Saravana Kumar Jaganathan Muthu Vignesh Vellayappan Gayathri Narasimhan Eko Supriyanto Dyah Ekashanti Octorina Dewi Aqilah Leela T Narayanan Arunpandian Balaji Aruna Priyadarshini Subramanian Mustafa Yusof | 2014 | World Journal of Gastroenterology2014，20，45： | 3 |
| 6 | Screening and Characterization of the Alkaline Protease Isolated from PLI-1, a Strain of Brevibacillus sp. Collected from Indonesia's Hot Springs显示文摘A total of 69 strains of thermophilic bacteria were isolated from water, soil and sediment samples from three Indonesia's hot spring areas (Pantai cermin, Kalianda and Banyu wedang) by using Minimal Synthetic Medium (MSM). The extreme thermophile Brevibacillus sp. PLI-1 was found to produce extracellular thermophilic alkaline protease with optimal activity at 70℃ and pH 8.0-9.0. The molecular weight of the protease was estimated to be around 56 kD by SDS-PAGE. The maximum activity of the protease was 26.54 U mL-1. The protease activity did not decrease after 30 min and still retained more than 70% of relative activity after 60 min at 70℃ and pH 8.0. The ion Mg2+ was found to promote protease activity at both low and high concentrations, whereas Cu2+ and Zn2+ could almost completely inhibit the activity. Divalent cation chelator EDTA inhibited the enzyme activity by 55.06% ± 0.27%, while the inhibition caused by PMSF, Leupeptin, Pepstain A and Benzamidine were 66.78% ± 3.25%, 52.37% ± 0.25%, 62.47% ± 2.96% and 50.99% ± 0.24%, respectively. Based on these observations, the enzyme activity was conspicuously sensitive to the serine and cysteine protease inhibitors. All these results indicated that the protease isolated from the strain PLI-1 was a thermophilic protease and had a high-temperature stability and a pH stability. | WANG Shuai LIN Xuezheng HUANG Xiaohang ZHENG Li Dewi Seswita Zilda | 2012 | Journal of Ocean University of China2012，11，2： | 3 |
| 7 | Transposon mouse models to elucidate the genetic mechanisms of hepatitis B viral induced hepatocellular carcinoma显示文摘The major type of human liver cancer is hepatocellular carcinoma(HCC), and there are currently many risk factors that contribute to this deadly disease. The majority of HCC occurrences are associated with chronic hepatitis viral infection, and hepatitis B viral(HBV) infection is currently a major health problem in Eastern Asia. Elucidating the genetic mechanisms associated with HBV-induced HCC has been difficult due to the heterogeneity and genetic complexity associated with this disease. A repertoire of animal models has been broadly used to study the pathophysiology and to develop potential treatment regimens for HBVassociated HCC. The use of these animal models has provided valuable genetic information and has been an important contributor to uncovering the factors involved in liver malignant transformation, invasion and metastasis. Recently, transposon-based mouse models are becoming more widely used in liver cancer research to interrogate the genome by forward genetics and also used to validate genes rapidly in a reverse genetic manner. Importantly, these transposon-based rapid reverse genetic mouse models could become crucial in testing potential therapeutic agents before proceeding to clinical trials in human. Therefore, this review will cover the use of transposon-based mouse models toaddress the problems of liver cancer, especially HBVassociated HCC occurrences in Asia. | Amy P Chiu Barbara R Tschida Lilian H Lo Branden S Moriarity Dewi K Rowlands David A Largaespada Vincent W Keng | 2015 | World Journal of Gastroenterology2015，21，42： | 2 |
| 8 | Syngas Production from Lignite Coal Using K 2 CO 3 Catalytic Steam Gasification with Controlled Heating Rate in Pyrolysis Step显示文摘 | Dijan Supramono Dewi Tristantini Agustina Rahayu Ricky Kristanda Suwignjo David Hartono Chendra | 2014 | Procedia Chemistry2014，，： | 2 |
| 9 | The meaning of complementary, alternative and traditional medicine among the Indonesian psychology community: a pilot study显示文摘OBJECTIVE: Complementary, alternative and traditional medicine(CATM) is a new field, as well as a promising area of study and practice in psychology. It is important to research the cultural context and meaning of CATM, including its definitions and examples, among different communities of psychology because CATM's use is dependent on how it is understood by the members. The aim of this pilot study is to provide an interpretation of the Indonesian psychology community's understanding of CATM through a qualitative approach.METHODS: Online interviews with open-ended questions and purposive sampling were used.Participants were dominantly psychologists or lecturers in clinical psychology area. Ten males and 12 females with an average age of 28.0 ± 2.5 years voluntarily participated in this study. Interviews were audio-recorded, transcribed verbatim, and reviewed and analysed by the two authors to ensure accuracy of interpretation.RESULTS: It was found that there was no single meaning of CATM among the Indonesian community of psychology. Participants were not familiar enough with the terms and tended to use them with overlap.It can be suggested that 'complementary medicine' and 'alternative medicine' or 'complementaryalternative medicine' combined provides more suitable terminology for use among Indonesian psychology community when communicating with other health care professionals.CONCLUSION: The understanding of the terms and examples of CATM were diverse and were often used interchangeably in the projects/interviews. It was also found that Indonesian psychologists required more education regarding CATM. In addition, future studies with more participants from various aspects of the psychology community should be conducted to capture a more representative sample. | Andrian Liem Kuncoro Dewi Rahmawati | 2017 | Journal of Integrative Medicine2017，15，4： | 2 |
| 10 | 结合类别关键词与注意力机制的药物相互关系抽取模型显示文摘为了增加对不同类别样例的区分度,提高模型的分类效果,提出了结合类别关键词和注意力机制的药物相互关系(DDI)抽取模型KA-BERT。首先基于卡方检验和文档频率获取每个类别的关键词,然后在预训练BERT模型中加入关键词与药物对的位置编码,以增加样例的差异性,并通过注意力机制学习关键词与句子中其他词的分布信息。针对药物关系抽取任务中负样例较多的问题,文中提出了基于规则和模式的负样例过滤方法,以有效降低正负样本比例。与现有基于CNN、基于LSTM和基于BERT的DDI提取模型实验结果的对比表明,KA-BERT模型能够很好地提高药物关系的提取效果,证明了该模型的有效性。在化学-蛋白质相互关系抽取上的测试结果表明,KA-BERT模型的准确率、召回率和F 1值均有明显的提升,证明了该模型的有效性和通用性。 | IKA Novita Dewi 蔡晓玲 刘晓锋 董守斌 | 2021 | 华南理工大学学报(自然科学版)2021，49，1： | 2 |
| 11 | Stroke rehabilitation:analysis of repeated Barthel index measures显示文摘 | Granger CV Dewis LS Peters NC | 1979 | Arch Phys Med Rehabil1979，60，： | 1 |
| 12 | Neuropeptides, food intake and body weight regulation: A hypothalamic focus显示文摘 | HILLEBRAND J J G DEWIED D ADAN R A H | 2002 | Peptides2002，23，： | 1 |
| 13 | Central target for the behavioural effects of vasopressin neuropeptides显示文摘 | Dewied D Gafforio Vanree J M | 1984 | Nature1984，308，： | 1 |
| 14 | Stroke rehabilitation: analysis of repeated Barthel index measures 显示文摘 | Granger C V Dewis L S Peters N C | 1979 | Arch Phys Med Rehabil1979，60，1： | 1 |
| 15 | Minimal processing and edible coating effects on composition and sensory quality of mini-peeled carrots显示文摘 | Howard L R Dewi T | 1996 | Journal of Food Science1996，61，3： | 1 |
| 16 | Prognostic effect of weight loss prior to chemotherapy in cancer patients 显示文摘 | DeWys WD Egg C Lavin PT | 1980 | Am J Med1980，69，4： | 1 |
| 17 | Intestinal Intraepithelial Lymphocytes Sustain the Epithelial BarrierFunction against Eimeria vermiformis Infection显示文摘 | Kyoko Inagaki-Ohara Fitriya Nurannisa Dewi Hajime Hisaeda | 2006 | Infection and immunity2006，74，9： | 1 |
| 18 | Requirement for ERK1/2 activation in the regulation of progesterone production in human granulosa-lutein cells is stimulus specific显示文摘 | Dewi D A Abayasekara D R E Wheeler-Jones C P D | 2001 | J Clin Endocrinol Metab2001，86，： | 1 |
| 19 | Prognostic effect of weight loss prior to chemotherapy in cancer patients显示文摘 | Dewys WD | 1980 | Am J Med1980，，： | 1 |
| 20 | Separation of isomers by ultrafiltration using modified cyclodextrins 显示文摘 | Yudiarto A Dewi E Kokugan T | 2000 | Separation and Purification Technology2000，19，： | 1 |
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