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您的检索式：作者名="Devajit Nath"
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| 1 | Immunofluorescence on paraffin embedded renal biopsies:Experience of a tertiary care center with review of literature显示文摘AIM To describe the technique of immunofluorescence on paraffin embedded tissue sections and discuss the potential pitfalls with an in depth review of literature.METHODS Immunofluorescence is integral to diagnostic renal pathology. Immunofluorescence on paraffin embedded renal biopsies(IF-P) after enzyme treatment has been described in literature, however has not found widespread use in renal pathology laboratories. In our laboratory proteinase K digestion of paraffin embedded renal biopsy material was standardized and applied prospectively in cases where immunofluorescence on fresh frozen tissue was non contributory or not possible. Diagnostic utility was assessed and in a cohort of cases comparison of intensity of staining with routine immunofluorescence was performed. RESULTS Over the 5-year study period, of the 3141 renal biopsies received IF-P was performed on 246 cases(7.7%) and was interpretable with optimal digestion in 214 cases(6.8%). It was of diagnostic utility in the majority of cases, which predominantly included glomerular disease. Nondiagnostic IF-P was found in membranous nephropathy(2 of 11 cases), membranoproliferative glomerulonephritis(2 of 32 cases), lupus nephritis(1 of 25 cases), post infectious glomerulonephritis(1 of 11 cases) and chronic glomerulonephritis(3 of 8 cases). Comparing cases with both routine IF and IF-P, 35 of 37 showed either equal intensity or a minor difference in intensity of staining(1+) for the diagnostic immunoglobulin/complement. Technically assessment of immunofluorescence on the paraffin embedded tissue was found to be easier with clearly observed morphology, however a false positive staining pattern was observed in under-digested tissue. CONCLUSION As a 'salvage' technique, immunofluorescence on paraffin embedded renal biopsies is of great diagnostic utility, however not without pitfalls. | Geetika Singh Lavleen Singh Ranajoy Ghosh Devajit Nath Amit Kumar Dinda | 2016 | World Journal of Nephrology2016，5，5： | 3 |
| 2 | Intraparotid facial nerve schwannoma: A case report显示文摘Facial nerve schwannoma occurring within the parotid gland is a rare tumour. We report a case of schwannoma within the parotid gland in a young female patient, who underwent ultrasound and magnetic resonanceimaging(MRI) and subsequent surgical excision of the lesion. The lesion showed hyperintensity on T2-weighted and diffusion-weighted MRI. There was no adjacent lymphadenopathy. Although hyperintensity on diffusionweighted MRI could suggest malignant tumours, the characteristic 'string sign' provided the clue for the diagnosis of schwannoma. | Abhishek Jaiswal Asit Ranjan Mridha Devajit Nath Ashu Seith Bhalla Alok Thakkar | 2015 | World Journal of Clinical Cases2015，3，3： | 1 |
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