| 1 | Preparation,structural characterization and bioactivities of polysaccharides from mulberry(Mori Fructus)显示文摘Mulberry(Mori Fructus),a highly promising functional food,has long exhibited multiple therapeutic effects as a tonic in traditional Chinese food and pharmacology.Polysaccharides have been regarded as the pivotal pharmacological ingredient in mulberry.In this study,structural characterization and bioactivities of polysaccharides from mulberry(PFM-3)were determined.The results showed that PFM-3 was a pyranose containing carboxyl group,which was mainly composed of glucose(Glc)and a small amount of mannose(Man)and fucose(Fuc).The molecular weight of PFM-3 was 96.17 kDa,and the surface of PFM-3 was compact and uniform crack.PFM-3 markedly decreased lipid accumulation,triglyceride(TG)and low density lipoprotein cholesterin(LDL-C)on hyperlipidemia human hepatocellular carcinoma(HepG2)cells.PFM-3 recovered nuclear morphometry and mitochondrial membrane potential(MMP),enhanced the activity of glutathione peroxidase(GPx)and decreased cell arrest in G2 phase on oxidative stress HepG2 cells.In addition,PFM-3 promoted the decline of tumor necrosis factor-α(TNF-α)and the augment of interleukin-10(IL-10)secretion on inflammatory mouse leukemia cells of monocyte macrophage(RAW 264.7).This work provides a certificate that PFM-3 could markedly improve cellular lipid accumulation,endogenous antioxidant defense capacity and inflammation levels to further use for treatment and amelioration of related diseases. | Yumeng Liu Yiming Liu Delun Mu Hui Yang Ying Feng Ruiqin Ji Rina Wu Junrui Wu | 2022 | Food Bioscience2022，46，2： | 1 |
| 2 | Encapsulation of docosahexaenoic acid (DHA) using self-assembling food-derived proteins for efficient biological functions显示文摘Docosahexaenoic acid(DHA;22n-6)possesses multiple biological functions, including antioxidant activity and ameliorating hypertriglyceridemia. However, the application of DHA has been limited due to poor aqueous solubility and susceptible to oxidation. Here, ovalbumin(O), myosin(M), 7S soy globulin(S), and β-lactoglobulin(β), hydrolyzed by chymotrypsin, self-assembled into micelles, respectively. Adding incremental DHA to micelles caused endogenous fluorescence quenching of O, M, S, and β micelles, implying successful incorporation of DHA into hydrophobic cores of micelles(O(DHA), M(DHA), S(DHA), and β(DHA)). The results showed that micelles provided spatial stability and improved solubility, and stability against thermal and ultraviolet(UV)light for DHA. The uptake of DHA from M(DHA), β(DHA), O(DHA), and S(DHA)was 3.27-, 3.91-, 2.7-, and 3.95-fold higher, respectively, than that of DHA by Caco-2 cells. Encapsulation in micelles increased DHA aqueous solubility and uptake, which enhanced cellular endogenous antioxidant defense. Meanwhile, increased uptake of DHA was verified by HepG2 cells, and O, M, S, and β micelles were proven to increase DHA uptake to reduce lipid deposition. Our findings strongly support the possibility that O, M, S, and β micelles can be regarded as a carrier for loading DHA. | Yumeng Liu Kaixin Cao Tong Li Delun Mu Naiqi Zhang Ying Wang Rina Wu Junrui Wu | 2023 | Food Science and Human Wellness2023，12，5： | 0 |
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