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| 1 | Liver fat deposition and mitochondrial dysfunction in morbid obesity:An approach combining metabolomics with liver imaging and histology显示文摘AIM: To explore the usefulness of magnetic resonance imaging(MRI) and spectroscopy(MRS) for assessment of non-alcoholic fat liver disease(NAFLD) as compared with liver histological and metabolomics findings. METHODS: Patients undergoing bariatric surgery following procedures involved in laparoscopic sleeve gastrectomy were recruited as a model of obesityinduced NAFLD in an observational, prospective, singlesite, cross-sectional study with a pre-set duration of 1 year. Relevant data were obtained prospectively and surrogates for inflammation, oxidative stress and lipid and glucose metabolism were obtained through standard laboratory measurements. To provide reliable data from MRI and MRS, novel procedures were designed to limit sampling variability and other sources of error using a 1.5T Signa HDx scanner and protocols acquired from the 3D or 2D Fat SAT FIESTA prescription manager. We used our previously described 1H NMRbased metabolomics assays. Data were obtained immediately before surgery and after a 12-mo period including histology of the liver and measurement of metabolites. Values from 1H NMR spectra obtained after surgery were omitted due to technical limitations.RESULTS: MRI data showed excellent correlation with the concentration of liver triglycerides, other hepatic lipid components and the histological assessment, w h i c h e xc l u d e d t h e p r e s e n c e o f n o n-a l c o h o l i c steatohepatitis(NASH). MRI was sufficient to follow up NAFLD in obese patients undergoing bariatric surgery and data suggest usefulness in other clinical situations. The information provided by MRS replicated that obtained by MRI using the-CH3 peak(0.9 ppm), the-CH2- peak(1.3 ppm, mostly triglyceride) and the-CH=CH- peak(2.2 ppm). No patient depicted NASH. After surgery all patients significantly decreased their body weight and steatosis was virtually absent even in patients with previous severe disease. Improvement was also observed in the serum concentrations of selected variables. The most relevant findings using metabolomics indicate increased levels of triglyceride and monounsaturated fatty acids in severe steatosis but those results were accompanied by a significant depletion of diglycerides, polyunsaturated fatty acids, glucose-6-phosphate and the ATP/AMP ratio. Combined data indicated the coordinated action on mitochondrial fat oxidation and glucose transport activity and may support the consideration of NAFLD as a likely mitochondrial disease. This concept may helpto explain the dissociation between excess lipid storage in adipose tissue and NAFLD and may direct the search for plasma biomarkers and novel therapeutic strategies. A limitation of our study is that data were obtained in a relatively low number of patients.CONCLUSION: MRI is sufficient to stage NAFLD in obese patients and to assess the improvement after bariatric surgery. Other data were superfluous for this purpose. | Nahum Calvo Raúl Beltrán-Debón Esther Rodríguez-Gallego Anna Hernández-Aguilera Maria Guirro Roger Mariné-Casadó Lidón Millá Josep M Alegret Fàtima Sabench Daniel del Castillo María Vinaixa Miguelàngel Rodríguez Xavier Correig Roberto García-álvarez Javier A Menendez Jordi Camps Jorge Joven | 2015 | World Journal of Gastroenterology2015，21，24： | 2 |
| 2 | Cost- t of ventilator- associated pneumonia with linezolid or vancomycin in Spain显示文摘 | Grau S Alvarez-Lerma F del Castillo effectiveness analysis of the treatmen A | 2005 | J Chemother2005，17，2： | 1 |
| 3 | On the functional form of the speed-density relationship - I : general theory显示文摘 | DEL CASTILLO J M BENITEZ F G | 1995 | Transportation Research B1995，29，5： | 1 |
| 4 | On the functional form of the speed-density relationship:general theory显示文摘 | Del Castillo J M Benitez F G | 1995 | Transportation Research Part B1995，29，5： | 1 |
| 5 | Phytoplankton assemblages in two compartmentalized coastal tropical lagoons(Carretas-Pereyra and ChantutoPanzacola,Mexico)显示文摘 | Varona-Cordero F Gutiérrez-Mendieta F J Del Castillo M E M | 2010 | Journal of Plankton Research2010，32，9： | 1 |
| 6 | On the functional form of the speed-density relationship - II : Empirical investigation显示文摘 | DEL CASTILLO J M BENITEZ F G | 1995 | Transportation Research B1995，29，5： | 1 |
| 7 | Potential of particle and fibre reinforcement of tyre tread elastomers显示文摘 | RJOS S CHICUREL R DEL CASTILLO L F | 2001 | Materials and Dcsign2001，22，： | 1 |
| 8 | Mononucleosis caused by Epstein-barr virus and cytomegalovirus in children: a comparative study of 124 cases显示文摘 | Lao A Borgue C Del Castillo F | 1994 | Pediatr Infect Dis J1994，13，1： | 1 |
| 9 | On the functional form of the speed-density relationship-I: General theory-II: Empirical investigation显示文摘 | Del Castillo J M Bennitez F G | 1995 | Transporation Research1995，29，： | 1 |
| 10 | On functional form of the speed-density relationship显示文摘 | Del Castillo J M Bennitez F G | 1995 | Transportation Research B1995，29，： | 1 |
| 11 | Influence of Peak Expiratory Flow Monitoring on an Asthma Self-Management Education Programme 显示文摘 | Lopez V A Del Castillo A F | 2000 | Respiratory Medicine2000，94，8： | 1 |
| 12 | Acute acquired lymphadenitis due to toxoplasmosis 显示文摘 | Del Castillo Martin F Funes Monux RM Gutierrez J | 1998 | An Esp Pediatr1998，49，1： | 1 |
| 13 | Motion data processing and wobbling mass modeling in the inverse dynamics of skeletal models 显示文摘 | Alonso F J Del Castillo J M Pintado P | 2006 | Mechanism and Machine Theory2006，8，6： | 1 |
| 14 | Prevalence and evolutionary origins of the del(GJB6>D13S1830) mutation in the DFNB1 locus in hearing-impaired subjects:a multicenter study显示文摘 | DEL CASTILLO I MORENO-PELAYO M A DEL CASTILLO F J | 2003 | Am J Hum Genet2003，73，： | 1 |
| 15 | Experimental metabolic surgery :justification and technical aspects显示文摘 | Sabench Pereferrer F Hemmdez Gonzez M Del Castillo D6jardin D | 2011 | Obes Surg2011，21，10： | 1 |
| 16 | Changes in antioxidant activity of dehydrated onion and garlic during storage显示文摘 | F Javier Moreno Marts Corzo-Martinez M Dolores del Castillo | 2006 | Food Research International2006，39，： | 1 |
| 17 | Trans-septal endoscopic approach of pituitary tnmors显示文摘 | Munoz del Castillo F Jurado Ramos A De la Riva Aguilar A | 2003 | Acta Otorrinolaringol Esp2003，54，8： | 1 |
| 18 | Construction and characterization of mutations at eodon 751 of the Escherichia coli gyrB gene that confer resistance to the antimicrobial peptide microcin BI7 and alter the activity of DNA gyrase 显示文摘 | del Castillo FJ del Castillo I Moreno F | 2001 | Journal of Bacteriology2001，183，6： | 1 |
| 19 | Response to planting distance and pruning system in tomatos growing in hydroponic culture in a basic greenhouse 显示文摘 | Hemandez Gonzalez V M F Sanchez Del Castillo | 1992 | Revista Chapingo1992，15，7374： | 1 |
| 20 | Neural reorganization accompanying upper limb motor rehabilitation from stroke with virtual reality-based gesture therapy显示文摘 | Orihuela-Espina F Fernández del Castillo I Palafox L | 2013 | Top Stroke Rehabil2013，20，3： | 1 |
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