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| 1 | Serum and urine metabolomic fingerprinting in diagnostics of inflammatory bowel diseases显示文摘AIM:To evaluate the utility of serum and urine metabolomic analysis in diagnosing and monitoring of inflammatory bowel diseases(IBD).METHODS:Serum and urine samples were collected from 24 patients with ulcerative colitis(UC),19 patients with the Crohn’s disease(CD)and 17 healthy controls.The activity of UC was assessed with the Simple Clinical Colitis Activity Index,while the activity of CD was determined using the Harvey-Bradshaw Index.The analysis of serum and urine samples was performed using proton nuclear magnetic resonance(NMR)spectroscopy.All spectra were exported to Matlab for preprocessing which resulted in two data matrixes for serum and urine.Prior to the chemometric analysis,both data sets were unit variance scaled.The differences in metabolite fingerprints were assessed using partial least-squaresdiscriminant analysis(PLS-DA).Receiver operating characteristic curves and area under curves were used to evaluate the quality and prediction performance of the obtained PLS-DA models.Metabolites responsible for separation in models were tested using STATISTICA10 with the Mann-Whitney-Wilcoxon test and the Student’s t test(α=0.05).RESULTS:The comparison between the group of patients with active IBD and the group with IBD in remission provided good PLS-DA models(P value 0.002for serum and 0.003 for urine).The metabolites that allowed to distinguish these groups were:N-acetylated compounds and phenylalanine(up-regulated in serum),low-density lipoproteins and very low-density lipoproteins(decreased in serum)as well as glycine(increased in urine)and acetoacetate(decreased in urine).The significant differences in metabolomic profiles were also found between the group of patients with active IBD and healthy control subjects providing the PLS-DA models with a very good separation(P value<0.001 for serum and 0.003 for urine).The metabolites that were found to be the strongest biomarkers included in this case:leucine,isoleucine,3-hydroxybutyric acid,N-acetylated compounds,acetoacetate,glycine,phenylalanine and lactate(increased in serum),creatine,dimethyl sulfone,histidine,choline and its derivatives(decreased in serum),as well as citrate,hippurate,trigonelline,taurine,succinate and 2-hydroxyisobutyrate(decreased in urine).No clear separation in PLS-DA models was found between CD and UC patients based on the analysis of serum and urine samples,although one metabolite(formate)in univariate statistical analysis was significantly lower in serum of patients with active CD,and two metabolites(alanine and N-acetylated compounds)were significantly higher in serum of patients with CD when comparing jointly patients in the remission and active phase of the diseases.Contrary to the results obtained from the serum samples,the analysis of urine samples allowed to distinguish patients with IBD in remission from healthy control subjects.The metabolites of importance included in this case up-regulated acetoacetate and down-regulated citrate,hippurate,taurine,succinate,glycine,alanine and formate.CONCLUSION:NMR-based metabolomic fingerprinting of serum and urine has the potential to be a useful tool in distinguishing patients with active IBD from those in remission. | Tomasz Dawiskiba Stanislaw Deja Agata Mulak Adam Zabek Ewa Jawień Dorota Pawelka Miroslaw Banasik Agnieszka Mastalerz-Migas Waldemar Balcerzak Krzysztof Kaliszewski Jan Skóra Piotr Bar Krzysztof Korta Kornel Pormańczuk Przemyslaw Szyber Adam Litarski Piotr Mlynarz | 2014 | World Journal of Gastroenterology2014，20，1： | 15 |
| 2 | CO 2 emissions from Polish cement industry显示文摘 | Jan Deja Alicja Uliasz-Bochenczyk Eugeniusz Mokrzycki | 2010 | International Journal of Greenhouse Gas Control2010，，4： | 1 |
| 3 | Antibiotic consumption and resis-tance: data from Europe and Germany 显示文摘 | Meyer E Gastmeier P Deja M | 2013 | Int J Med Microbiol2013，303，67： | 1 |
| 4 | Whole body hyperthermia: a secure procedure for patients with various malignancies显示文摘 | Kemer T Deja M Ahlers O | 1999 | Int- ensive Care Med1999，25，： | 1 |
| 5 | Immobilization of Cr6+,Cd2+,Zn2+ and Pb2+ in Alkali-activated Slag Binders显示文摘 | DEJA J | 2002 | Cement and Concrete Research2002，32，12： | 1 |
| 6 | Prevention measures of ventilator-associ- ated pneumonia显示文摘 | Deja M Spies C | 2009 | Crit Care Med2009，37，1： | 1 |
| 7 | Whole body hyperthermia: a secure procedure for patients with various malignancies显示文摘 | Kemer T Deja M Ahlers O | 1999 | Int- ensive Care Med1999，25，： | 1 |
| 8 | Immobilization of Cr6+, Cd2+, Zn2+ and Pb2+ in alkali-activated slag binders显示文摘 | Jan Deja | 2002 | Cement and Concrete Research2002，32，： | 1 |
| 9 | Impact of bolus application of low-dose hydrocortisone on glycemic control in septic shock patients显示文摘 | Weber-Carstens S Deja M Bercker S Dimroth A | 2007 | Intensive Care Med2007，33，4： | 1 |
| 10 | Evaluation of selected serum protein markers as early detectors of ovarian cancer显示文摘 | Mrochem J Sodowski K Deja R | 2008 | Ginekol Pol2008，79，4： | 1 |
| 11 | Evaluation of selected serum protein markers as early detectors of ovarian cancer显示文摘 | Mrochem J Sodowski K Deja R | 2008 | Ginekol Pol2008，79，4： | 1 |
| 12 | Spectroscopic studies of alkaline activated slag geopolymers显示文摘 | Mozgawa W Deja J | 2009 | Journal of Molecular Structure2009，，： | 1 |
| 13 | Monitoring arterial blood pressure during whole body hyperthermia显示文摘 | Kerner T Deja M Ahlers O | 2002 | Acta Anaesthesiol Scand2002，46，5： | 1 |
| 14 | Coronary-artery bypass surgery in patients with left ventricular dysfunction 显示文摘 | Velazquez EJ Lee KL Deja MA | 2011 | N Engl J Med2011，364，17： | 1 |
| 15 | Monitoring arterial blood pressure during whole body hyperthermia 显示文摘 | Kemer T Deja M Ahlers O | 2002 | Aeta Anaesthesiol Seand2002，46，5： | 1 |
| 16 | Whole body hyperthermia: a secure procedure for patients with various malignancies? 显示文摘 | Kerner T Deja M Ahlers O | 1999 | Intensive Care Med1999，25，9： | 1 |
| 17 | Monitoring arterial blood pressure during whole body hyperthermia 显示文摘 | Kerner T Deja M Ahlers O | 2002 | Acta Anaesthesiol Scand2002，46，5： | 1 |
| 18 | Evaluation of selected serumprotein markers as early detectors of ovarian cancer显示文摘 | Mrochem J Sodowski K Deja R eta1 | 2008 | Ginekol Pol2008，79，4： | 1 |
| 19 | Effects of preoperative aspirin in coronary artery bypass grafting: a double-blind, placebo-controlled, randomized trial 显示文摘 | Deja MA Kargul T Domaradzki W | 2012 | J Thoracic Cardiovasc Surg2012，144，： | 1 |
| 20 | Risk factors in critical illness myopathy during the early course of critical illness:a rospective observational study显示文摘 | Weber-Carstens S Deja M Koch S | 2010 | Crit Care2010，14，3： | 1 |
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