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| 1 | Relationship between Fusobacterium nucleatum,inflammatory mediators and microRNAs in colorectal carcinogenesis显示文摘AIM To examine the effect of Fusobacterium nucleatum(F. nucleatum) on the microenvironment of colonic neoplasms and the expression of inflammatory mediators and microRNAs(miRNAs).METHODS Levels of F. nucleatum DNA, cytokine gene mRNA(TLR2, TLR4, NFKB1, TNF, IL1 B, IL6 and IL8), and potentially interacting miRNAs(miR-21-3p, miR-22-3p, mi R-28-5p, miR-34a-5p, miR-135b-5p) were measured by quantitative polymerase chain reaction(qPCR) TaqMan? assays in DNA and/or RNA extracted from the disease and adjacent normal fresh tissues of 27 colorectal adenoma(CRA) and 43 colorectal cancer(CRC) patients. KRAS mutations were detected by direct sequencing and microsatellite instability(MSI) status by multiplex PCR. Cytoscape v3.1.1 was used to construct the postulated miRNA:mRNA interaction network.RESULTS Overabundance of F. nucleatum in neoplastic tissue compared to matched normal tissue was detected in CRA(51.8%) and more markedly in CRC(72.1%). We observed significantly greater expression of TLR4, IL1 B, IL8, and miR-135 b in CRA lesions and TLR2, IL1 B, IL6, IL8, mi R-34 a and miR-135 b in CRC tumours compared to their respective normal tissues. Only two transcripts for miR-22 and miR-28 were exclusively downregulated in CRC tumour samples. The mRNA expression of IL1 B, IL6, IL8 and miR-22 was positively correlated with F. nucleatum quantification in CRC tumours. The mRNA expression of miR-135 b and TNF was inversely correlated. The miRNA:mRNA interaction network suggested that the upregulation of miR-34 a in CRC proceeds via a TLR2/TLR4-dependent response to F. nucleatum. Finally, KRAS mutations were more frequently observed in CRC samples infected with F. nucleatum and were associated with greater expression of miR-21 in CRA, while IL8 was upregulated in MSI-high CRC.CONCLUSION Our findings indicate that F. nucleatum is a risk factor for CRC by increasing the expression of inflammatory mediators through a possible mi RNA-mediated activation of TLR2/TLR4. | Marcela Alcantara Proenca Joice Matos Biselli Maysa Succi Fábio Eduardo Severino Gustavo Noriz Berardinelli Alaor Caetano Rui Manuel Reis David J Hughes Ana Elizabete Silva | 2018 | World Journal of Gastroenterology2018，24，47： | 15 |
| 2 | Mucins MUC1, MUC2, MUC5AC and MUC6 expressin in the evaluation of differentiation and clinico-biological behaviour of gastric carcinoma显示文摘 | Pinto-de-Sousa J David L Reis CA | 2002 | Virchows Arch2002，440，3： | 2 |
| 3 | SAP155-Mediated Splicing of FUSE-Binding Protein-Interacting Repressor Serves as a Molecular Switch for c-myc Gene Expression显示文摘 | Kazuyuki Matsushita Toshiko Kajiwara Mai Tamura Mamoru Satoh Nobuko Tanaka Takeshi Tomonaga Hisahiro Matsubara Hideaki Shimada Rei Yoshimoto Akihiro Ito Shuji Kubo Tohru Natsume David Levens Minoru Yoshida Fumio Nomura | 2012 | Molecular Cancer Research2012，，6： | 2 |
| 4 | Topographic expression of MUC5AC and MUC6 in the gastric mucosa infected by Helicobacter pylori and in associated diseases显示文摘 | Terezinha Marques Leonor David Celso Reis Ana Nogueira | 2005 | Pathology - Research and Practice2005，，10： | 1 |
| 5 | Immunohistochemical study of MUCSAC expression in human gastric carcinomas using a novel monoclonal antibody 显示文摘 | REIS C A DAVID L NIELSEN P A | 1997 | Int J Cancer1997，74，： | 1 |
| 6 | Mucins MUC1,MUC2,MUC5AC and MUC6 expression in the evaluation of differentiation and clinico-biological behavior of gastric carcinoma显示文摘 | Pinto-de-Sousa J David L Reis C A | 2002 | Virchows Arch2002，440，3： | 1 |
| 7 | The cardiovascular aspects of a Ménière’s disease population——A pilot study显示文摘Introduction:MD is considered a rare disease.An adequate model that explains MD's pathophysiology is not well established.Recently,the vascular theory of the disease has been revived.Objectives:To characterize a MD population according to its cardiovascular risk and correlate it to the MD clinical course.Methods:In this retrospective chart study the data of 31 MD patients observed between January 2017 and April 2018 in a tertiary university hospital were reviewed.Patients included in the study were diagnosed according to the Barany Society criteria.Lost follow-ups,patients with autoimmune disease,atopy or allergy,major psychiatric disease and migraine were excluded.Age,gender,cardiovascular risk factors,audiometric and vestibular parameters,occurrence of MD attacks in the previous 6 months,vestibular medication in course and time course of MD were recorded and compared between groups(with and without cardiovascular risk factors).Results:31 patients(61.3%females)mean aged 60.3 years(±9.7)were studied.74%of the population had at least one risk factor and 51.6%of patients had attacks in the last 6 months.There was a statistically significant difference in the occurrence of MD attacks in the last 6 months(p=0.014)between MD patients with and without risk factors.Mean PTA thresholds were higher and speech discrimination was lower in individuals with more cardiovascular risk factors.Conclusions:Treatment of MD focusing on vascular risk factors may allow a better control of symptoms and result in a decreased need for ablative procedures in this disorder. | Angela Reis Rego David Dias Ana Pinto SANDra Sousa e Castro Telma Feliciano Cecília Almeida e Sousa | 2019 | Journal of Otology2019，14，2： | 1 |
| 8 | Intestinal metaplasia of human stomach displays distinct of mucin(MUC1,MUC2,MUC5AC and MUC6) expression 显示文摘 | David L Correa P | 1999 | Cancer Res1999，59，5： | 1 |
| 9 | Immunohistochemical study of MUC5AC expression in human gastric carcinomas using a novel monoclonal antibody显示文摘 | Reis CA David L Nielsen PA | 1997 | Int J Cancer1997，74，1： | 1 |
| 10 | Intestinal metaplasia of human stomach displays distinct patterns lf mucin(Muc1,Muc2,Muc5AC,Muc6)expression显示文摘 | Reis CA David L Correa P | 1999 | Cancer Res1999，59，5： | 1 |
| 11 | Mucins MUC1,MUG2,MUC5AC and MUG6 exprseeion in the evaluation of differentiation and clinico-biological behaviour of gastric carcinoma显示文摘 | Pinto-de-Sousa J David L Reis CA | 2002 | Virchows Arch2002，440，： | 1 |
| 12 | Immunohistochemical study of MUC5AC expression in human gastric carcinomas using a novel monoclonal antibody显示文摘 | Reis CA David L Nielsen PA | 1997 | Int J Caner1997，74，1： | 1 |
| 13 | Immunohisto-chemical study of the expression of MUC6 mucin and co-expression of other secreted mucins (MUC5A and MU C2) in human gastric carcinomas 显示文摘 | David L Caralho F | 2000 | J Histochem Cytochem2000，48，3： | 1 |
| 14 | Mucins MUC1,MUC2,MUC5AC and MUC6 expression in the evaluation of differentiation and clinico-biological behaviour of gastric carcinoma 显示文摘 | Pinto-de-Sousa J David L Reis CA | 2002 | Virchows Arch2002，440，3： | 1 |
| 15 | Mucins MUC1, MUC2, MUC5AC and MUC6 expression in the evaluation of differentiation and clinico- biological behaviour of gastric carcinoma 显示文摘 | Pinto-de-Sousa J David L Reis C A | 2002 | Virchows Arch2002，440，3： | 1 |
| 16 | Immunohistochemical study of MUC5AC expression in human gastric carcinomas using a novel monoclonal antibody显示文摘 | Reis CA David L Nielsen PA | 1997 | Int J Cancer1997，74，： | 1 |
| 17 | Expression of mucins (MUC1, MUC2, MUC5 AC, and MUC6) and type 1 Lewis antigens in cases with and without Helicobacter pylori colonization in metaplastic glands of the human stomach 显示文摘 | Teixeira A David L Reis CA | 2002 | Pathol2002，197，1： | 1 |
| 18 | Topographic expression of MUC5AC and MUC6 in the gastric mucosa infected by Helicobacter pylori and in associated diseases 显示文摘 | Marques T David L Reis C | 2005 | Pathol Res Pract2005，201，10： | 1 |
| 19 | Expression of fully and underglycosylated forms of MUC1 mucin in gastric carcinoma显示文摘 | David L Seixas M | 1998 | Int J Cancer1998，79，： | 1 |
| 20 | Immunohistochemical study of MUC5AC expression in human gastric carcinomas using a novel monoclonal antibody显示文摘 | Reis CA David L Ntelsen PA Clausen H Mirgorodskaya K Roepstorff P | 1997 | Int J Cancer1997，74，1： | 1 |
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