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| 1 | Highly active antiretroviral therapy dysregulates proliferation and differentiation of human pre-adipocytes显示文摘AIM To investigate the mechanism(s) by which potential effects of multi-drug highly-active antiretroviral therapy contributes to lipodystrophy syndrome. METHODS Preadipocytes from healthy donors were assessed for proliferation and differentiation in the presence of nucleoside reverse transcriptase inhibitors(NRTIs), nonnucleoside reverse transcriptase inhibitors(NNRTIs), and protease inhibitors(PIs) individually and in combination. Effects on proliferation were assessed with a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay and effects on differentiation were assessed from glycerol-3-phosphate dehydrogenase(GPDH) activity and quantitation of Oil Red O staining for intracellular lipid. Data were analyzed with a randomized block ANOVA with post-hoc Fisher's Least Significant Difference test. RESULTS Preadipocyte proliferation was inhibited by a combination of NNRTI + NRTI(14% at 48 h, P < 0.001) and PI + NRTI(19% at 48 h, P < 0.001) with additional suppression when ritonavir(RTV) was added(26% at 48 h). The drug combination of atazanavir(ATV) + RTV + emtricitabine(FTC) + tenofovir(TDF) had the greatest inhibitory effect on proliferation at 48 h. Preadipocyte differentiation was most significantly reduced by the efavirenz + FTC + TDF assessed either by GPDH activity(64%) or lipid accumulation(39%), P < 0.001. Combining NRTIs with a PI(ATV + FTC + TDF) significantly suppressed differentiation(GPDH activity reduced 29%, lipid accumulation reduced by 19%, P < 0.01). This effect was slightly greater when a boosting amount of RTV was added(ATV + FTC + TDF + RTV, P < 0.001). CONCLUSION Although combination antiretroviral therapy is clinically more efficacious than single drug regimens, it also has a much greater inhibitory effect on preadipocyte proliferation and differentiation. | Eyone Jones Pavel Mazirka Margaret A McNurlan Frank Darras Marie C Gelato Giuseppe Caso | 2017 | World Journal of Virology2017，6，3： | 3 |
| 2 | Accessibility in post secondary education: Application of UDL to college curriculum显示文摘 | Darra Pace Diane Schwartz | 2008 | 美中教育评论2008，5，12： | 2 |
| 3 | Origin of mutations in two families with X-linked chronic granulomatous disease显示文摘 | Francke U Ochs HD Darras BT | 1990 | Blood1990，76，3： | 1 |
| 4 | Reoperation and ercurrence of plemorphic adenoma of the parotid显示文摘 | Chevolier D Loche V Darras JA | 1996 | Ann Otolaryngol Chir Cervicofac1996，113，2： | 1 |
| 5 | Surgical management of sinonasal inverted papillomas through endoscopic approach显示文摘 | MORTUAIRE G ARZUL E DARRAS J A | 2007 | Eur Arch Otorhinolaryngol2007，264，： | 1 |
| 6 | Involve- ment of thyroid hormones in chicken embryonic brain develop- ment 显示文摘 | Darras V M Van Herck S L Geysens S | 2009 | Gen Comp Endocrinol2009，163，12： | 1 |
| 7 | 查看详情显示文摘 | Darras V Fichet O Perrot F Boileau S Teyssié D | | 0，，： | 1 |
| 8 | Spinal mt~scular atrophy: a clinical and research update显示文摘 | Markowitz JA Singh P Darras BT | 2012 | Pediatr Neurol2012，46，： | 1 |
| 9 | Polysiloxane-poly(fluorinated acrylate ) interpenetrating polymer networks: synthesis and characterization 显示文摘 | Darras V Fichet O Perrot F | 2007 | Polymer2007，48，： | 1 |
| 10 | Friction stir processing of commercial AZ31 magnesium alloy 显示文摘 | Darras B M Khraisheh M K Abu-Farha F K | 2007 | Journal of Materials Processing Technology2007，19，： | 1 |
| 11 | Distribution and regulation of chicken growth hormone secretagogue receptor isoforms 显示文摘 | Geelissen S M Beck I M Darras V M | 2003 | Gen Comp Endocrinol2003，134，2： | 1 |
| 12 | Human ventricular/slow twitch myosin alkali light chain gene characterization sequence and chromosomal localiza tion显示文摘 | Fodor WL Darras B Seharaseyon J Falkenthal S Francke U Vanin EF | 1989 | J Biol Chem1989，26，： | 1 |
| 13 | Identification of a neuroprotective and selective butyrylcholinesterase in- hibitor derived from the natural alkaloid evodiamine显示文摘 | Huang GZ Kling B Darras FH | 2014 | European Journal of Medicinal Chemistry2014，81，： | 1 |
| 14 | Ghrelin in birds:its structure,distribution and function显示文摘 | KAIYA H DARRAS V M KANGAWA K | 2007 | The Journal of Poultry Science2007，44，： | 1 |
| 15 | Evaluation of Cervical Spine in Intensive Care Patients following Blunt Trauma显示文摘 | Roxie M. Albrecht Darra Kingsley Carol R. Schermer Gerald B. Demarest Edward C. Benzel Blaine L. Hart | 2001 | World Journal of Surgery2001，，8： | 1 |
| 16 | Urologic considerations and complications in kidney transplant recipients显示文摘 | Di Carlo HN Darras FS | 2015 | Adv Chronic Kidney Dis2015，22，4： | 1 |
| 17 | Growth of heat-shock induced triploids of bluetilapia,Oreochromis aureus,reared in tanks and in ponds in Eastern Congo:feeding regimes and compensatory growth response of triploid females显示文摘 | Byamungu N Darras V M Kuhn E R | 2001 | Aquaculture2001，198，12： | 1 |
| 18 | Type 2 iodothyronine deiodinase protein in chicken choroid plexus: additional perspectives on T3 supply in the avian brain 显示文摘 | Verhoelst CH Darras VM Roelens SA | 2004 | J Endocrinol2004，183，1： | 1 |
| 19 | Epithelioid hemangioen- dothelioma of the liver 显示文摘 | Darras T Moisse R Colette JM | 1988 | J Beige Radiol1988，71，6： | 1 |
| 20 | Nosocomial cerebral aspergillosis: a report of 3 cases显示文摘 | Darras JC VeberB Bedos JP | 1996 | Scand J Infect Dis1996，28，： | 1 |
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