4篇
您的检索式：作者名="Darning Ren"
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| 1 | Expression, purification of a synthetic fuse-protein-TSF from PF4 and TSP1 fragments and its effect on angio-genesis and tumor growth显示文摘Sequences encoding PF4 (58-70) and TSP1 (429-459) were linked to yield a single gene TSF which encodes the fuse-protein of TSF. The gene was cloned into a pGEX-2T expression vector to generate a protein GST-TSF, which was strongly expressed in E. coli. The purified GST-TSF was degraded with thrombin to generate the protein TSF. With the methods of MTT and wound repair assay, the effects of TSF on the proliferation and migration of EC were detected, respectively. The results showed that TSF significantly suppressed BAEC proliferation and migration in a dose-dependent manner. The fuse protein GST-TSF, and the peptides PF4 (58-70) and TSP1 (429-459) also inhibited BAEC proliferation and migration, respectively, but their inhibition rates were not as high as TSF. Using the CAM assay, it was shown that TSF, GST-TSF, PF4 (58-70) and TSP1 (429-459) inhibited angiogenesis in chick CAM potentially, the effect of TSF was the highest. In vivo, the growth of Lewis lung carcinoma was potently inhibited by TSF | SHEN Xianrong JI Dongmei HU Youqing JIA Fuxing DENG Xinxian WANG Ling CHU Zhiyong JIANG Dingwen LEI Chengxiang HUANG Jiansheng HU Xinran REN Darning | 2001 | Chinese Science Bulletin2001，46，24： | 2 |
| 2 | Genetic studies of A2M and BACEI genes in Chinese Han Alzheimer's disease patients显示文摘 | Lei Cai Guomei Tang Lishan Chen Bosheng Zhang Sanduo Jiang Darning Ren | 2005 | 中国生物学文摘2005，19，11： | 1 |
| 3 | Prophylaxis of tumor through oral administration of IL-12 GM-CSF gene carried by live attenuated salmonella显示文摘A live attenuated AraA autotrophic mutant of Salmonella typhimurium (SL3261) was used as carrier for eukaryotic expression vectors EGFPN1, pCMVmIL-12, pCMVWL-12, pCMVmGM-CSF and PCMVhGM-CSF and was administered orally to BALB/c and C57BL/6 mice. After 6 weeks, these mice were challenged with 4T1 and Lewis tumor cells respectively. GFP expression and gene integration could be detected in mice’s livers, spleens, intestines, kidneys and tumors. The serum level of cytokines increasedsignificantly in treated mice, so did the ratio of CD8+/CD4+,which resulted in the tumor regression and prolongation of the survival time of those mice. These researches laid an experimental foundation for the tumor gene therapy using live attenuated salmonella. | LI Yuhua CHEN Hui QUO Kunyuan XIE Yongmei WANG Feng XIE Kuangcheng HUANG Jiangsheng REN Darning | 2001 | Chinese Science Bulletin2001，46，13： | 1 |
| 4 | A 0.20–2.43 GHz fractional-N frequency synthesizer with optimized VCO and reduced current mismatch CP显示文摘A 0.20–2.43 GHz fractional-N frequency synthesizer is presented for multi-band wireless communication systems,in which the scheme adopts low phase noise voltage-controlled oscillators(VCOs)and a charge pump(CP)with reduced current mismatch.VCOs that determine the out-band phase noise of a phase-locked loop(PLL)based frequency synthesizer are optimized using an automatic amplitude control technique and a high-quality factor figure-8-shaped inductor.A CP with a mismatch suppression architecture is proposed to improve the current match of the CP and reduce the PLL phase errors.Theoretical analysis is presented to investigate the influence of the current mismatch on the output performance of PLLs.Fabricated in a TSMC 0.18-μm CMOS process,the prototype operates from 0.20 to 2.43 GHz.The PLL synthesizer achieves an in-band phase noise of-96.8 dBc/Hz and an out-band phase noise of-122.8 dBc/Hz at the 2.43-GHz carrier.The root-mean-square jitter is 1.2 ps under the worst case,and the measured reference spurs are less than-65.3 dBc.The current consumption is 15.2 mA and the die occupies 850μm×920μm. | Wei ZOU Darning REN Xuecheng ZOU | 2021 | Frontiers of Information Technology & Electronic Engineering2021，22，2： | 0 |
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