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您的检索式：作者名="Danjun Fang"
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| 1 | Develop a 3D neurological disease model of human cortical glutamatergic neurons using micropillar-based scaffolds显示文摘Establishing an effective three-dimensional(3D) in vitro culture system to better model human neurological diseases is desirable, since the human brain is a 3D structure. Here, we demonstrated the development of a polydimethylsiloxane(PDMS) pillar-based 3D scaffold that mimicked the 3D microenvironment of the brain. We utilized this scaffold for the growth of human cortical glutamatergic neurons that were differentiated from human pluripotent stem cells. In comparison with the 2D culture, we demonstrated that the developed 3D culture promoted the maturation of human cortical glutamatergic neurons by showing significantly more MAP2 and less Ki67 expression. Based on this 3D culture system,we further developed an in vitro disease-like model of traumatic brain injury(TBI), which showed a robust increase of glutamate-release from the neurons, in response to mechanical impacts, recapitulating the critical pathology of TBI. The increased glutamate-release from our 3D culture model was attenuated by the treatment of neural protective drugs, memantine or nimodipine. The established 3D in vitro human neural culture system and TBI-like model may be used to facilitate mechanistic studies and drug screening for neurotrauma or other neurological diseases. | Cheng Chen Xin Dong Kai-Heng Fang Fang Yuan Yao Hu Min Xu Yu Huang Xixiang Zhang Danjun Fang Yan Liu | 2019 | Acta Pharmaceutica Sinica B2019，9，3： | 3 |
| 2 | Weakly coordinating group directed rhodium-catalyzed unconventional site-selective C-H olefination of indolizines at the 8-position显示文摘A rhodium-catalyzed directing group promoted selective C-H olefination reaction of indolizines at the 8-position is re ported.Di-olefination at 2,8-positions also achieved with silver hexafluoroantimonate as an additive under similar reaction conditions.Weakly coordinating groups,such as ketone,alde hyde,amide and ester,were used as directing groups.The ester group can be removed under acid conditions and therefore is used as a traceless directing group. | Xue Feng Jiaxin Tian Ying Sun Huayou Hu Mingzhu Lu Yuhe Kan Danjun Fang Chao Wang | 2021 | Chinese Chemical Letters2021，32，1： | 0 |
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