| 1 | In Vitro Characterizations of PLLA/β-TCP Porous Matrix Materials and RMSC-PLLA-β-TCP Composite Scaffolds显示文摘To develop a novel degradable poly (L-lactic acid)/β-tricalcium phosphate (PLLA/β-TCP) bioactive materials for bone tissue engineering, β-TCP powder was produced by a new wet process. Porous scaffolds were prepared by three steps, I.e. Solvent casting, compression molding and leaching stage. Factors influencing the compressive strength and the degradation behavior of the porous scaffold, e.g. Weight fraction of pore forming agent-sodium chloride (NaCl), weight ratio of PLLA: β-TCP, the particle size ofβ-TCP and the porosity, were discussed in details. Rat marrow stromal cells (RMSC) were incorporated into the composite by tissue engineering approach. Biological and osteogenesis potential of the composite scaffold were determined with MTT assay, alkaline phosphatase (ALP) activity and bone osteocalcin (OCN) content evaluation. Results show that PLLA/β-TCP bioactive porous scaffold has good mechanical and pore structure with adjustable compressive strength needed for surgery. RMSCs seeding on porous PLLA/β-TCP composite behaves good seeding efficacy, biocompatibility and osteoinductive potential. Osteoprogenitor cells could well penetrate into the material matrix and begin cell proliferation and osteogenic differentiation. Osseous matrix could be formed on the surface of the composite after culturing in vitro. It is expected that the PLLA/β-TCP porous composites are promising scaffolds for bone tissue engineering in prosthesis surgery. | DaliZHOU WeizhongYANG GuangfuYIN ChangqiongZHENG YunZHANG HuaiqingCHEN RuiCHEN | 2004 | Journal of Materials Science & Technology2004，20，3： | 3 |
| 2 | Osteogenesis and Degradation Behavior of rhBMP-2/β-Tricalcium Phosphate Porous Composite Materials显示文摘Ultrafine -tricalcium phosphate (β-TCP) powders with good crystalline structure were produced by a new wet process. Throughbone tissue engineering approach, porous β-TCP ceramic was combined with recombined human bone morphogenetic proteins- 2 (rhBMP-2) to develop a novel composite material. Osteogenesis capacity of the composite was investigated intramuscularlyin rat with histological analyses and SEM examination. Pure β-TCP porous ceramic was investigated as the control. Resultsshow that the composite materials possess good biocompatibility, biodegradation and strong osteogenesis capacity throughinductive process after implantation. Material degradation began from 2 weeks post-implantation accompanying with thechanging of pore structure, with the enwrapping and separation of materials by hyperplatic mesenchymal cells and fibroblast,and with the phagocytose reaction of multinucleated giant cells. Early in 72 h, immature cartilage could be found within novelcomposite; mature lamellar bone was induced to generate after 3 weeks. With strong osteoinduction capacity and controllablebiodegradation, the novel rhBMP-2/β-TCP porous ceramic is expected to be a promising bone grafting substitute for bonetissue engineering. | WeizhongYANG DaliZHOU GeLIANG GuangfuYIN YunZHANG | 2004 | Journal of Materials Science & Technology2004，20，6： | 1 |
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