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| 1 | Subcellular localization of the stripe disease-specific protein encoded by rice stripe virus (RSV) in its vector,the small brown planthopper,Laodel-phax striatellus显示文摘The stripe disease-specific protein (SP) encoded by the rice stripe virus (RSV) was successfully used as a localization signal of the virus in its vector, the small brown lanthopper, Laodelphax striatellus Fallen. Immunogold particles in large numbers were detected in various parts of the viruliferous females: the ovum, surface of chorion, the mid-gut lumen, and the columnar cells. Whereas there was noneof these particles in the non-viruliferous females and males, and testis of viruliferous males. Endosymbionts (mycetocytes) were abundant, harboring ovaries of both viruliferous and non-viruliferous females, but none in the testis of males. The results provided us with the direct proof that RSV is a ciruculative and propagative plant virus and it was transovarially transmitted alongside with endosymbionts of its vector. Therefore, we deem it a nice lead for future studies on the mechanism of RSV transmission and functioning of its viral proteins. | WU Aizhong ZHAO Yan QU Zhicai SHEN Daleng PAN Chongguang SU Deming | 2001 | Chinese Science Bulletin2001，46，21： | 23 |
| 2 | Cloning,expression and function of phosphate transporter encoded gene in Oryza sativa L.显示文摘OsPT6:1,a phosphate transporter encoding gene from the leaf samples of Oryza sativa, was identified through PCR with specifically designed primers.The phylogenetic analysis and the conserved amino acid residue site detection suggested OsPT6:1 a possible high-affinity phosphate transporter encoding gene.In situ hybridization and RT-PCR demonstrated the expression of OsPT6:1 in both roots and leaves.The peak expression signal was observed in mesophyll cells under low phosphorus(P)induction.A homologous recombination study indicated that OsPT6:1 can enhance the Pi uptake efficiency of Pichia pastoris.At the meantime,the introduction of OsPT6:1 was able to complement the Pi uptake function of yeast cells with high-affinity phosphate transporters de- ficient.Those results substantiated our contention that OsPT6:1 encoded a high-affinity phosphate transporter of Oryza sativa. | MING Feng 1,2* ,LU Qun 1,2* ,WANG Wei 1,2* ,ZHANG Shanshan 1,2 ,GUO Bin 1,2 &SHEN Daleng 1,2 1.Institute of Genetics,State Key Laboratory of Genetic Engineering,School of Life Science,Fudan University,Shanghai 200433,China 2.Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering Institute of Biodiversity Science, School of Life Science,Fudan University,Shanghai 200433,China | 2006 | Science China(Life Sciences)2006，49，5： | 4 |
| 3 | Cloning, Molecular Characterization, and Application of Rice Epiphytic Bacillus pumilus Promoter Fragments显示文摘 | Qingyu Cao Zhicai Qu Youzhong Wan Hongwei Zhang Daleng Shen | 2001 | Current Microbiology2001，，4： | 1 |
| 4 | Cloning,expression and molecular characterization of promoter elements from Bacillus pumilus显示文摘Promoter elements from random chromosomal DNA of a rice epiphytic Bacillus pumilus were cloned into promoter probe shuttle vector ECE7 and sequenced. The results showed that these elements were all new DNA sequences. Six strong promoter elements were obtained by determination of CAT enzyme activity in both E. coli and B. pumilus. Transcription start sites of the cat mRNA were located by primer extension using total RNA. Comparison of the promoter sequences indicated that three of them contain -10 and -35 regions like B. pumilus σ43 consensus sequence and another one is similar to B. pumilus σ29. The other two have no typical consensus sequences of known sigma factors so far. | Qingyu Cao Zhicai Qu Youzhong Wan Hongwei Zhang Daleng Shen Jiazhen Tan | 2001 | Chinese Science Bulletin2001，46，9： | 0 |
| 5 | 通过危害度分析和多指标决策改进维修管理显示文摘挪威电力部门的维修工作是以动态生产为依据。维修策略是以平衡预防维修与改正性维修为目标。通过RCM分析制订维修方案,提高了维修管理水平。对维修项目按经济指标和质量指标进行评估。 | Jφrn Egil Johnsen Jan Daleng 王秩信 | 2008 | 设备管理与维修2008，，9： | 0 |
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