|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Study the effects of divalent metallic ions on the combination of DNA and histones with fluorescence anisotropy assays显示文摘The effects of divalent ions(Mn2+,Mg2+,Ca2+) on the interaction between DNA and histone are studied using a fluorescence anisotropy assay. Fluorescence anisotropies of DNA and DNA-histone in the presence of divalent ions(Mn2+,Mg2+,Ca2+) are measured. The results indicate that histone reduces the fluorescence anisotropy of lambda DNA while the divalent ions(Mn2+,Mg2+,Ca2+) significantly enhance the fluorescence anisotropy. Compared to the case of DNA incubated with histone alone,there are more histones binding to DNA when divalent ion,histone and DNA are incubated together. We also find that Mn2+ makes the DNA-histone complexes more condensed than the other ions do. | LIU YuYing WANG PengYe DOU ShuoXing XIE Ping XI XuGuang | 2007 | Chinese Science Bulletin2007，52，9： | 4 |
| 2 | Local conformation transitions of linear DNA induced by cisplatin显示文摘Cisplatin is the most successful anti-tumor drug,and its pharmacological property is generally considered to derive from the modification of DNA molecules.Structural modifications of short DNA induced by cisplatin have already been investigated.However,the conformation transitions induced by cisplatin are not clear.In the present letter,we have studied the effect of low-concentration cisplatin on DNA conformation by using AFM imaging.We observed formations of micro-rod structures of linear DNA induced by cisplatin.A method is presented to quantitatively analyze the occurrence of micro-rod structures.We found that the formation of micro-rod structures depends on the DNA sequence.Based on the results,we proposed a physical mechanism to explain the local conformation transitions of DNA molecules under the influence of cisplatin. | Chao Ji Lingyun Zhang Shuoxing Dou Pengye Wang | 2014 | Chinese Science Bulletin2014，59，25： | 1 |
| 3 | Ionic effect on combing of single DNA molecules and observation of their force-induced melting by fluorescence microscopy显示文摘 | LIU Yuying WANG Pengye DOU Shuoxing | 2004 | J Chem Phys2004，121，： | 1 |
| 4 | The histone octamer influences the wrapping direction of DNA on it:Brownian dynamics simulation of the nucleosome chirality显示文摘 | LI Wei DOU Shuoxing WANG Pengye | 2005 | J Theor Biol2005，235，： | 1 |
| 5 | Single-molecule studies of DNA by molecular combing显示文摘分子的梳是为排列 DNA 分子的一个大数组到表面上的一个强大的方法。它是 DNA 分子被力量经由液体流动在玻璃表面上拉长并且排列的一个过程。在一个单个封面上梳直到几百 DNA 的能力滑倒允许要成为的大小的一个统计上重要的数字。这些特征成为分子为 genomic 研究梳一个吸引人的工具,例如 DNA 复制, DNA 抄写, DNA 蛋白质相互作用等等。在这篇评论文章,我们讨论分子的梳原则方法和它的应用。 | Liu Yuying Wang Pengye Dou Shuoxing | 2007 | Progress in Natural Science:Materials International2007，17，5： | 0 |
| 6 | Kinetic study of the DNA annealing properties of RECQ5β helicase显示文摘RecQ family helicases are critical for maintaining genomic integrity. Many RecQ family helicases not only unwind duplex, and other more complicated DNA structures, but also possess, interestingly, DNA annealing (strand pairing) activity. Here, we systematically investigated the DNA annealing properties of RECQ5β by measuring DNA annealing kinetics, equilibrium DNA binding, and kinetics of dissociation from ssDNA. RECQ5β catalyzed DNA annealing most efficiently when the enzyme molecules covered approximately 40%-50% of the DNA strand, in the absence or presence of different nucleotide cofactors (AMPPNP, ATPγS, or ADP) under our buffer conditions. A comparative study with RECQ5β1-662 confirmed that the C-terminal region of RECQ5β was essential for its high DNA annealing activity. These results contribute to our understanding of the mechanism of DNA annealing catalyzed by RecQ family helicases. | DING XiuYan XU YaNan LI Wei WANG PengYe XI XuGuang DOU ShuoXing | 2012 | Chinese Science Bulletin2012，57，11： | 0 |
| 7 | Monitoring the binding of metal cations and histones to DNA in real time using fluorescence assays显示文摘The binding of cations (Na +,K +,Mg 2+,Ca 2+,Mn 2+) and histones to DNA can be studied using fluorescence assays.Here,we measured the fluorescence intensity and fluorescence anisotropy of DNA and DNA-histone complexes in the presence of cations.We demonstrate that when different cations are added into a DNA solution,the fluorescence intensities of the stained DNA are reduced by different amounts.Compared with divalent cations,monovalent cations had a weaker effect on fluorescence intensity and fluorescence anisotropy.Divalent (Mn 2+,Mg 2+,Ca 2+) cations markedly enhanced the fluorescence anisotropy of DNA.The binding modes of monovalent and divalent cations to DNA may be different.Divalent cations can change the structure of DNA molecules,or promote the assembly of DNA strands.The addition of histones causes DNA condensation,which mostly occurs during the first few seconds.Cation binding to DNA is abrupt,and is much faster than that of histones. | LIU YuYing WANG PengYe DOU ShuoXing ZHANG WeiWei WANG XueJin SANG HongYi | 2011 | Chinese Science Bulletin2011，56，11： | 0 |
| 8 | Study of the interaction of DNA and histones by spin-stretching and droplet evaporation显示文摘Molecular combing is a powerful and simple method for aligning DNA molecules onto a surface. Using this technique combined with fluorescence microscopy, DNA-histone complexes are stretched on a hydrophobic polymethyl methacrylate (PMMA) surface and observed directly. We have developed a new method to stretch single DNA-histone complexes, termed spin-stretching. The results show that the histones markedly enhance DNA binding to the PMMA surface. DNA winds around the histones and therefore decreases in length. The number of histones that bind to each DNA molecule is found to correlate with the histone concentration. The combed DNA-histone complexes are found to depend on two factors: the binding force on the surface and the centrifugal force at its local position. Na+ ions should compete with histones for binding to DNA; however, the observed competitive binding effect of Na+ ions at low concentrations was negligible. | LIU YuYing WANG PengYe DOU ShuoXing ZHANG WeiWei WANG XueJin SANG HongYi | 2011 | Chinese Science Bulletin2011，56，12： | 0 |
| 9 | Spermidine-induced two-dimensional DNA condensations on mica surfaces:A different pathway from condensations in solution显示文摘With atomic force microscopy (AFM) we systematically studied the DNA condensations on mica surfaces induced by multivalent cation spermidine. The pattern of the DNA condensates is a flat single layer, with a core in the centre and DNA wrapping around it at high density. We assume this to be a two-dimensional condensation of free coiled DNA onto negatively charged mica surfaces by the multivalent cation. The DNA molecules condense on mica surfaces via a pathway different from the formation of toroids, rods or globules in bulk solutions. We give an explanation to why toroid structures are difficult to be observed by AFM, and further discuss the relationship between DNA condensations in solutions and on mica surfaces. The present work will be helpful for understanding the behaviors of DNA on charged surfaces, which might be significantly different from that in solutions. | ZHANG XingHua HOU XiMiao JI Chao LIMing DOU ShuoXing WANG PengYe | 2009 | Chinese Science Bulletin2009，54，14： | 0 |
| 10 | Studying the interaction between gyrase and DNA using magnetic tweezers显示文摘The DNA gyrase of Escherichia coli plays an essential role in the life of this microorganism.It is unique among all topoisomerases because of its ability to introduce negative supercoils into DNA.This study investigated the single molecular interaction of E.coli gyrase with DNA using magnetic tweezers.The results showed that,in the absence of ATP,gyrase weakly binds the G and T segments.The stretched force of 0.7 pN can gradually destroy the binding,whereas that of 5.9 pN directly destroys it.Addition of high concentrations of norfloxacin enhances gyrase binding to both segments,making them adapt to 5.9 pN.DNA gyrase reduces the plectonemic dimension,which was determined by the bacterial enzyme and not by the pull force.Moreover,it has different affinities for positive supercoils,which it prefers,and negative supercoils.The time distribution of the dissociation of gyrase from DNA has a double-exponential form.We herein propose a model to explain this distribution and compare the results with those of other models. | ZHANG ZhiQiang LIU YuRu XIE Ping LI Wei DOU ShuoXing WANG PengYe | 2012 | Chinese Science Bulletin2012，57，27： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员