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| 1 | CDX2 protein expression compared to alcian blue staining in the evaluation of esophageal intestinal metaplasia显示文摘AIM:To compare the sensitivity and specificity of CDX2 and alcian blue(AB) p H 2.5 staining in identifying esophageal intestinal metaplasia.METHODS:One hundred and ninty-nine biopsies from 186 patients were retrospectively reviewed and categorized as Barrett's esophagus(BE)(n =108); non-Barrett's esophagus(NBE)(n =48); columnar blue cells(CB) and esophageal glands(EG)(n =43).The biopsies were stained with AB and immunostained for CDX2 using a mouse monoclonal antibody from Biogenex(clone CDX2-88) and the Ventana Discovery X automated immunostainer.The positive and negative predictive value of each group was used to determine the predictive power of CDX2 and AB in diagnosing intestinal metaplasia.RESULTS:All of the 108 BE biopsies(100%) were positive for AB and 102 of them(94.4%) were positive for CDX2.The six BE patients(5.6%) who failed to stain with CDX2 were found to have lost the focus of intestinal metaplasia upon deeper sectioning for immunostaining.Both AB and CDX2 were negative in 43 out of 48(89.6%) NBE cases.Five NBE patients(10.4%) were falsely positive for AB due to the presence of EG and CB in these biopsies.These cases were all CDX2 negative.In addition,5 AB negative NBE were found to be CDX2 positive.Based on these results the CDX2 immunostain had similar sensitivity but higher specificity(100% vs about 91%) than AB in detecting intestinal type metaplasia in these samples.Our data shows that CDX2 has a better PPV in detecting intestinal metaplasia as compared to AB(95.6% vs 71.5%,respectively).CONCLUSION:CDX2 has a better positive predictive value than AB in detecting intestinal metaplasia.CDX2may be useful when challenged by gastro-esophageal biopsies containing mimikers of BE. | Dennis R Johnson Maisoun Abdelbaqui Maryam Tahmasbi Zoltan Mayer Hung-Wei Lee Mokenge P Malafa Domenico Coppola | 2015 | World Journal of Gastroenterology2015，21，9： | 2 |
| 2 | Anti-tumor necrosis factor α therapy associates to type 17 helper T lymphocytes immunological shift and significant microbial changes in dextran sodium sulphate colitis显示文摘BACKGROUND Anti-tumor necrosis factor α(TNFα) represents the best therapeutic option to induce mucosal healing and clinical remission in patients with moderate-severe ulcerative colitis. On the other side gut microbiota plays a crucial role in pathogenesis of ulcerative colitis but few information exists on how microbiota changes following anti-TNFα therapy and on microbiota role in mucosal healing.AIM To elucidate whether gut microbiota and immune system changes appear following anti TNFα therapy during dextran sulfate sodium(DSS) colitis.METHODS Eighty C57 BL/6 mice were divided into four groups: 'No DSS', 'No DSS + antiTNFα', 'DSS' and 'DSS + anti-TNFα'. 'DSS' and 'DSS + anti-TNFα' were treated for 5 d with 3% DSS. At day 3, mice whithin 'No DSS+anti-TNFα' and'DSS+anti-TNFα' group received 5 mg/kg of an anti-TNFα agent. Forty mice were sacrificed at day 5, forty at day 12, after one week of recovery post DSS. The severity of colitis was assessed by a clinical score(Disease Activity Index), colon length and histology. Bacteria such as Bacteroides, Clostridiaceae, Enterococcaceae and Fecalibacterium prausnitzii(F. prausnitzii) were evaluated by quantitative PCR.Type 1 helper T lymphocytes(Th1), type 17 helper T lymphocytes(Th17) and CD4+ regulatory T lymphocytes(Treg) distributions in the mesenteric lymph node(MLN) were studied by flow cytometry.RESULTS Bacteria associated with a healthy state(i.e., such as Bacteroides, Clostridiaceae and F. prausnitzii) decreased during colitis and increased in course of anti-TNFαtreatment. Conversely, microorganisms belonging to Enterococcaceae genera,which are linked to inflammatory processes, showed an opposite trend.Furthermore, in colitic mice treated with anti-TNFα microbial changes were associated with an initial increase(day 5 of the colitis) in Treg cells and a consequent decrease(day 12 post DSS) in Th1 and Th17 frequency cells. Healthy mice treated with anti-TNFα showed the same histological, microbial and immune features of untreated colitic mice. 'No DSS + anti-TNFα' group showed a lymphomononuclear infiltrate both at 5 th and 12 th d at hematoxylin and eosin staining, an increase of in Th1 and Th17 frequency at day 12, an increase of Enterococcaceae at day 5, a decrease of Bacteroides and Clostridiaceae at day 12.CONCLUSION Anti-TNFα treatment in experimental model of colitis improves disease activity but it is associated to an increase in Th17 pathway together with gut microbiota alteration. | Valentina Petito Cristina Graziani Loris R Lopetuso Marco Fossati Alessandra Battaglia Vincenzo Arena Domenico Scannone Gianluca Quaranta Andrea Quagliariello Federica Del Chierico Lorenza Putignani Luca Masucci Maurizio Sanguinetti Alessandro Sgambato Antonio Gasbarrini Franco Scaldaferri | 2019 | World Journal of Gastroenterology2019，25，12： | 2 |
| 3 | Pharmacology of nitric oxide:molecular mechanismsand therapeutic strategies显示文摘 | Domenico R | 2004 | Curr Pharm Des2004，10，： | 1 |
| 4 | Cardiac stem cells in patients with ischaemic cardiomy opathy(SCIPIO):initial results of a randomised phase 1 trial显示文摘 | Roberto Bdli Atul R Chugh Domenico D Amario | 2011 | Lancet2011，378，： | 1 |
| 5 | Enhancement of the centroiding algo- rithm for star tracker measure refineme 显示文摘 | Giancarl R Domenico A | 2003 | Acta Astronautlca2003，53，2： | 1 |
| 6 | Existenee and uniqueness for a nonlinear fractional differential equation显示文摘 | DOMENICO D LURGI R | 1996 | Journal of Mathematical Analysis and Applications1996，204，2： | 1 |
| 7 | The rabbit as an ex- perimental and production animal: From genomics to pro- teomics 显示文摘 | Ingrid M Claire R G Domenico S | 2014 | Current Protein Peptide Sci2014，15，2： | 1 |
| 8 | Pharmacology of nitric oxide: molecular mechanisms and therapeutic strategies 显示文摘 | Domenico R | 2004 | Curr Pharm Des2004，10，14： | 1 |
| 9 | Plasma and serum levels of D dimmer andtheir correlation with other haemostatic parameters in pregnancy显示文摘 | Domenico P Bandinelli B | 2002 | Thromb Res2002，105，： | 1 |
| 10 | Pharmacology of nitric oxide: molecular mechanisms and therapeutic strategies 显示文摘 | Domenico R | 2004 | Curr Pharm Des2004，10，14： | 1 |
| 11 | Papillary cystic and solid tumors of the pancreas: A pancreatic embryonic tumor? Studies of three cases and cumulative review of the world's literature? 显示文摘 | Mao C Guvendi M Domenico D R | 1995 | Surgery1995，118，5： | 1 |
| 12 | Effect of methylguanidine in a model of septic shock induced by LPS显示文摘 | Marzocco S Di Paola R Ribecco MT Sorrentino R Domenico B Genesio M | 2004 | Free Radic Res2004，38，： | 1 |
| 13 | Thermo-struc tural behaviour of an UHTC made nose cap of a reentry vehicle显示文摘 | Rosario B Aniello R Domenico T | | 0，，03： | 1 |
| 14 | Enhancement of the centroiding algorithm for star tracker measure refinement显示文摘 | GIANCARL R DOMENICO A | 2003 | ACTA Astronautica2003，50，： | 1 |
| 15 | Antioxidant statusand lipid peroxidation in patients with essential hypertension 显示文摘 | Clarla R Oliviero O Domenico G | 1998 | J hyper- tension1998，16，9： | 1 |
| 16 | Pharmacology of nitric oxide : molecular mechanisms and therapeutic strategies 显示文摘 | Domenico R | 2004 | Curt Pharm Des2004，10，14： | 1 |
| 17 | Protein levels of heat shock proteins 27, 32, 60, 70, 90and thioredoxin-1 in amnestic mild cognitive impairment: an investigation on the role of cellular stress response in the progression of Alzheimer disease 显示文摘 | Di Domenico F Sultana R Tiu GF | 2010 | Brain Res2010，1333，： | 1 |
| 18 | Pharmacology of nitric oxide: molecular mechanisms and therapeutic strategies 显示文摘 | Domenico R | 2004 | Curr Pharm Des2004，10，： | 1 |
| 19 | Anti-oxidant status and lipid peroxidation in patients with essential hypertension显示文摘 | Clarla R Oliviero O Domenico G | 1998 | J hypertension1998，16，9： | 1 |
| 20 | Papillary cystic and solid tumors of the pancreas:a pancreatic embryonic tumor? Studies of three cases and cumulative review of the world's literature显示文摘 | MAO C GUVENDI M DOMENICO D R | 1995 | Surgery1995，118，： | 1 |
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