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| 1 | Engineering of Yarrowia lipolytica for production of astaxanthin显示文摘Astaxanthin is a red-colored carotenoid,used as food and feed additive.Astaxanthin is mainly produced by chemical synthesis,however,the process is expensive and synthetic astaxanthin is not approved for human consumption.In this study,we engineered the oleaginous yeast Yarrowia lipolytica for de novo production of astaxanthin by fermentation.First,we screened 12 different Y.lipolytica isolates for β-carotene production by introducing two genes for β-carotene biosynthesis:bi-functional phytoene synthase/lycopene cyclase(crtYB)and phytoene desaturase(crtI)from the red yeast Xanthophyllomyces dendrorhous.The best strain produced 31.1±0.5 mg/L β-carotene.Next,we optimized the activities of 3-hydroxy-3-methylglutaryl-coenzyme A reductase(HMG1)and geranylgeranyl diphosphate synthase(GGS1/crtE)in the best producing strain and obtained 453.9±20.2 mg/L β-carotene.Additional downregulation of the competing squalene synthase SQS1 increased the β-carotene titer to 797.1±57.2 mg/L.Then we introduced β-carotene ketolase(crtW)from Paracoccus sp.N81106 and hydroxylase(crtZ)from Pantoea ananatis to convert β-carotene into astaxanthin.The constructed strain accumulated 10.4±0.5 mg/L of astaxanthin but also accumulated astaxanthin biosynthesis intermediates,5.7±0.5 mg/L canthaxanthin,and 35.3±1.8 mg/L echinenone.Finally,we optimized the copy numbers of crtZ and crtW to obtain 3.5 mg/g DCW(54.6 mg/L)of astaxanthin in a microtiter plate cultivation.Our study for the first time reports engineering of Y.lipolytica for the production of astaxanthin.The high astaxanthin content and titer obtained even in a small-scale cultivation demonstrates a strong potential for Y.lipolytica-based fermentation process for astaxanthin production. | Kanchana Rueksomtawin Kildegaard Belen Adiego-Perez David Domenech Belda Jaspreet Kaur Khangura Carina Holkenbrink Irina Borodina | 2017 | Synthetic and Systems Biotechnology2017，2，4： | 12 |
| 2 | Severe cyclophosphamide-related hyponatremia in a patient with acute glomerulonephritis显示文摘Cyclophosphamide is frequently used to treat cancer,autoimmune and renal diseases,such as rapidly progressive glomerulonephritis.Its side effects are well-known,including bone marrow depression,infections,alopecia,sterility,bladder malignancy and hemorrhagic cystitis.Moreover,in some cases cyclophosphamide use has been related to the onset of hyponatremia,by development of a syndrome of inappropriate antidiuresis.Indeed,severe hyponatremia has been previously reported in patients treated with high-dose or moderate-dose of intravenous cyclophosphamide,while only few cases have been reported in patients treated with low dose.Here,we discuss a case of a syndrome of inappropriate antidiuresis followed to a single low-dose of intravenous cyclophosphamide in a patient with a histological diagnosis of acute glomerulonephritis,presenting as acute kidney injury.After cyclophosphamide administration(500 mg IV),while renal function gradually improved,the patient developed confusion and headache.Laboratory examinations showed serum sodium concentration dropped to 122 mmol per liter associated with an elevated urinary osmolality of 199 mO sm/kg,while common causes of acute hyponatremia were excluded.He was successfully treated with water restriction and hypertonic saline solution infusion with the resolution of the electrolyte disorder.This case,together with the previous ones already reported,highlights that electrolyte profile should be strictly monitored in patients undergoing cyclophosphamide therapy in order to early recognize the potentially lifethreatening complications of acute water retention. | Pasquale Esposito Maria Valentina Domenech Nicoletta Serpieri Marta Calatroni Ilaria Massa Alessandro Avella Edoardo La Porta Luca Estienne Elena Caramella Teresa Rampino | 2017 | World Journal of Nephrology2017，6，4： | 2 |
| 3 | Black boxes in analytical chemistry: University students misconceptions of instrumental analysis显示文摘 | Antonio Domenech Carbo, Jose Vicente Gimeno Adelantado Francisco Bosch Reig | 2010 | 美中教育评论2010，7，7： | 2 |
| 4 | TiO2 deactivation during gas-phase photocatalytic oxidation of ethanol显示文摘 | PIERA E AYLLoN J A DOMeNECH X | 2002 | Catal Today2002，76，24： | 2 |
| 5 | Kinetics of the photocatalytic oxidation of N( HI ) and S( 1V ) on different semiconductor oxides显示文摘 | Domenech X Peral J | 1999 | Chemo- sphere1999，38，6： | 1 |
| 6 | Recombinant expres- sion of goat milk serum amyloid A: preliminary studies of the protein and derived peptides on macrophage phagocytosis 显示文摘 | DOMENECH A RAYNES JG RODRIQUEZ EG et a/ | 2012 | Protein and Peptide Let- ters2012，19，3： | 1 |
| 7 | Oculographicfindings in 145 patients with benign paroxysmal positional vertigo显示文摘 | Domenech Campos E Armen Got Carcell ER M Barona DE | 2006 | Acta Otorrinola ringol Esp2006，57，： | 1 |
| 8 | The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation显示文摘 | SANCHEZ C DOMENECH N VAZQUEZ J | 1999 | J Immunol1999，162，9： | 1 |
| 9 | TiO2 deactivation during the gas-phase photocatalytic oxidation of dimethyl sulfide显示文摘 | Gonzalez-Garcia N Ayllon JA Domenech X | 2004 | Applied Catalysis B:Environmental2004，52，1： | 1 |
| 10 | Heterogeneous photocatalysis for purification,decontamination and deodovization of air显示文摘 | Peral J Domenech X Ollis D F | 1997 | J Chem Technol Biotechnol1997，70，2： | 1 |
| 11 | External laryngeal trauma: Study of 12 cases 显示文摘 | Ayuso MA Luis M Domenech J | 1994 | Rev Esp Anestesiol Reanita1994，41，6： | 1 |
| 12 | L- asparaginase loaded red blood cells in refractory or relapsing acute lymphoblastic leukaemia in children and adults: results of the GRASPALL 2005-01 randomized trial显示文摘 | Domenech C Thomas X Chabaud S | 2011 | Br J Haematol2011，153，1： | 1 |
| 13 | Effects of stress by unfamiliar sounds on carcass and meat traits in bulls from three continental beef cattle breeds at different ageing times 显示文摘 | PENA F AVILES C DOMENECH V | 2014 | Meat Science2014，，98： | 1 |
| 14 | Analysis of cellular immune response in pigs recovered from porcine reproductive and respiratory syndrome infection 显示文摘 | Lopez F Domenech L N Alvarez B | 1999 | Virus Res1999，64，1： | 1 |
| 15 | Recombinant expression of goat milk serum amyloid A:preliminary studies of the protein and derived peptides on macrophage phagocytosis显示文摘 | Domenech A Raynes J G Rodriquez E G | | 0，，03： | 1 |
| 16 | Human capital inequality and economic growth:some new evidence显示文摘 | Castello A Domenech R | 2002 | The Economic Journal2002，，112： | 1 |
| 17 | Synthesis and characterization of polypyrrole/TiO2 composites on mild steel显示文摘 | Ferreira C A Domenech S C Lacaze P C | 2001 | J Appl Electrochem2001，31，： | 1 |
| 18 | Moderate exer- cise is an 'antioxidant:Upregulation of antioxidant genes by training显示文摘 | Gomez Cabrera MC Domenech E Vina J | 2008 | Free Radic Biol Med2008，44，2： | 1 |
| 19 | Two contiguously located gerraline BRCA1 mutations in a Spanish early- onset breast cancer family显示文摘 | Domenech M Cones J | 1999 | Cancer Lett1999，142，1： | 1 |
| 20 | A plant type 2 metallothionein(MT)from cork tissue responds to oxidative stress显示文摘 | Mir G Domenech J Huguet G | 2004 | J Exp Bot2004，55，408： | 1 |
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